Cargando…

Three-Dimensional (3D) Tumor Spheroid Invasion Assay

Invasion of surrounding normal tissues is generally considered to be a key hallmark of malignant (as opposed to benign) tumors. For some cancers in particular (e.g., brain tumors such as glioblastoma multiforme and squamous cell carcinoma of the head and neck – SCCHN) it is a cause of severe morbidi...

Descripción completa

Detalles Bibliográficos
Autores principales: Vinci, Maria, Box, Carol, Eccles, Suzanne A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542056/
https://www.ncbi.nlm.nih.gov/pubmed/25993495
http://dx.doi.org/10.3791/52686
_version_ 1782386481201938432
author Vinci, Maria
Box, Carol
Eccles, Suzanne A.
author_facet Vinci, Maria
Box, Carol
Eccles, Suzanne A.
author_sort Vinci, Maria
collection PubMed
description Invasion of surrounding normal tissues is generally considered to be a key hallmark of malignant (as opposed to benign) tumors. For some cancers in particular (e.g., brain tumors such as glioblastoma multiforme and squamous cell carcinoma of the head and neck – SCCHN) it is a cause of severe morbidity and can be life-threatening even in the absence of distant metastases. In addition, cancers which have relapsed following treatment unfortunately often present with a more aggressive phenotype. Therefore, there is an opportunity to target the process of invasion to provide novel therapies that could be complementary to standard anti-proliferative agents. Until now, this strategy has been hampered by the lack of robust, reproducible assays suitable for a detailed analysis of invasion and for drug screening. Here we provide a simple micro-plate method (based on uniform, self-assembling 3D tumor spheroids) which has great potential for such studies. We exemplify the assay platform using a human glioblastoma cell line and also an SCCHN model where the development of resistance against targeted epidermal growth factor receptor (EGFR) inhibitors is associated with enhanced matrix-invasive potential. We also provide two alternative methods of semi-automated quantification: one using an imaging cytometer and a second which simply requires standard microscopy and image capture with digital image analysis.
format Online
Article
Text
id pubmed-4542056
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MyJove Corporation
record_format MEDLINE/PubMed
spelling pubmed-45420562015-08-31 Three-Dimensional (3D) Tumor Spheroid Invasion Assay Vinci, Maria Box, Carol Eccles, Suzanne A. J Vis Exp Medicine Invasion of surrounding normal tissues is generally considered to be a key hallmark of malignant (as opposed to benign) tumors. For some cancers in particular (e.g., brain tumors such as glioblastoma multiforme and squamous cell carcinoma of the head and neck – SCCHN) it is a cause of severe morbidity and can be life-threatening even in the absence of distant metastases. In addition, cancers which have relapsed following treatment unfortunately often present with a more aggressive phenotype. Therefore, there is an opportunity to target the process of invasion to provide novel therapies that could be complementary to standard anti-proliferative agents. Until now, this strategy has been hampered by the lack of robust, reproducible assays suitable for a detailed analysis of invasion and for drug screening. Here we provide a simple micro-plate method (based on uniform, self-assembling 3D tumor spheroids) which has great potential for such studies. We exemplify the assay platform using a human glioblastoma cell line and also an SCCHN model where the development of resistance against targeted epidermal growth factor receptor (EGFR) inhibitors is associated with enhanced matrix-invasive potential. We also provide two alternative methods of semi-automated quantification: one using an imaging cytometer and a second which simply requires standard microscopy and image capture with digital image analysis. MyJove Corporation 2015-05-01 /pmc/articles/PMC4542056/ /pubmed/25993495 http://dx.doi.org/10.3791/52686 Text en Copyright © 2015, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial License, which permits non-commercial use, distribution, and reproduction, provided the original work is properly cited.
spellingShingle Medicine
Vinci, Maria
Box, Carol
Eccles, Suzanne A.
Three-Dimensional (3D) Tumor Spheroid Invasion Assay
title Three-Dimensional (3D) Tumor Spheroid Invasion Assay
title_full Three-Dimensional (3D) Tumor Spheroid Invasion Assay
title_fullStr Three-Dimensional (3D) Tumor Spheroid Invasion Assay
title_full_unstemmed Three-Dimensional (3D) Tumor Spheroid Invasion Assay
title_short Three-Dimensional (3D) Tumor Spheroid Invasion Assay
title_sort three-dimensional (3d) tumor spheroid invasion assay
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542056/
https://www.ncbi.nlm.nih.gov/pubmed/25993495
http://dx.doi.org/10.3791/52686
work_keys_str_mv AT vincimaria threedimensional3dtumorspheroidinvasionassay
AT boxcarol threedimensional3dtumorspheroidinvasionassay
AT ecclessuzannea threedimensional3dtumorspheroidinvasionassay