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Analysis of cerebrospinal fluid from cattle with central nervous system disorders after storage for 24 hours with autologous serum

BACKGROUND: We compared the changes in cell morphology, total and differential cell counts between cerebrospinal fluid (CSF) samples analyzed within an hour of collection (fresh sample) and after the addition of autologous serum and storage for 24 h (stored sample) in 27 cattle with central nervous...

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Autores principales: Bellino, C., Miniscalco, B., Bertone, I., Cagnasso, A., Occhiena, E., Gianella, P., D’Angelo, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542111/
https://www.ncbi.nlm.nih.gov/pubmed/26268228
http://dx.doi.org/10.1186/s12917-015-0502-x
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author Bellino, C.
Miniscalco, B.
Bertone, I.
Cagnasso, A.
Occhiena, E.
Gianella, P.
D’Angelo, A.
author_facet Bellino, C.
Miniscalco, B.
Bertone, I.
Cagnasso, A.
Occhiena, E.
Gianella, P.
D’Angelo, A.
author_sort Bellino, C.
collection PubMed
description BACKGROUND: We compared the changes in cell morphology, total and differential cell counts between cerebrospinal fluid (CSF) samples analyzed within an hour of collection (fresh sample) and after the addition of autologous serum and storage for 24 h (stored sample) in 27 cattle with central nervous system disorders. RESULTS: There was a positive linear correlation between total and differential cell counts in the fresh and the stored samples. Cell morphology was preserved in all stored samples, except for increased vacuolization of mononuclear cells and cleaved nuclei of some small mononuclear cells. In the stored CSF samples, the total nucleated cell count and monocyte percentage were decreased (P = 0.01; P = 0.03), while the lymphocyte percentage was increased (P = 0.04). Mononuclear pleocytosis diagnosed in 20 fresh samples was cytologically confirmed in 12 of the 20 stored samples. In the remaining eight stored samples, the number of total nucleated cells was within the normal range. Neutrophilic pleocytosis was confirmed in all seven stored samples. The overall agreement rate between cytologic interpretation of the fresh and the stored CSF samples was 70 % (100 % for neutrophilic pleocytosis and 60 % for mononuclear pleocytosis). CONCLUSIONS: Adding 11 % of autologous serum to CSF samples might allow delayed analysis with a good agreement rate for CSF cytological interpretation. Caution is nonetheless warranted, as animal age, anamnesis, and neurological presentation need to be considered when interpreting stored CSF without pleocytosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12917-015-0502-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-45421112015-08-21 Analysis of cerebrospinal fluid from cattle with central nervous system disorders after storage for 24 hours with autologous serum Bellino, C. Miniscalco, B. Bertone, I. Cagnasso, A. Occhiena, E. Gianella, P. D’Angelo, A. BMC Vet Res Research Article BACKGROUND: We compared the changes in cell morphology, total and differential cell counts between cerebrospinal fluid (CSF) samples analyzed within an hour of collection (fresh sample) and after the addition of autologous serum and storage for 24 h (stored sample) in 27 cattle with central nervous system disorders. RESULTS: There was a positive linear correlation between total and differential cell counts in the fresh and the stored samples. Cell morphology was preserved in all stored samples, except for increased vacuolization of mononuclear cells and cleaved nuclei of some small mononuclear cells. In the stored CSF samples, the total nucleated cell count and monocyte percentage were decreased (P = 0.01; P = 0.03), while the lymphocyte percentage was increased (P = 0.04). Mononuclear pleocytosis diagnosed in 20 fresh samples was cytologically confirmed in 12 of the 20 stored samples. In the remaining eight stored samples, the number of total nucleated cells was within the normal range. Neutrophilic pleocytosis was confirmed in all seven stored samples. The overall agreement rate between cytologic interpretation of the fresh and the stored CSF samples was 70 % (100 % for neutrophilic pleocytosis and 60 % for mononuclear pleocytosis). CONCLUSIONS: Adding 11 % of autologous serum to CSF samples might allow delayed analysis with a good agreement rate for CSF cytological interpretation. Caution is nonetheless warranted, as animal age, anamnesis, and neurological presentation need to be considered when interpreting stored CSF without pleocytosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12917-015-0502-x) contains supplementary material, which is available to authorized users. BioMed Central 2015-08-13 /pmc/articles/PMC4542111/ /pubmed/26268228 http://dx.doi.org/10.1186/s12917-015-0502-x Text en © Bellino et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (http://creativecommons.org/licenses/by/4.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Bellino, C.
Miniscalco, B.
Bertone, I.
Cagnasso, A.
Occhiena, E.
Gianella, P.
D’Angelo, A.
Analysis of cerebrospinal fluid from cattle with central nervous system disorders after storage for 24 hours with autologous serum
title Analysis of cerebrospinal fluid from cattle with central nervous system disorders after storage for 24 hours with autologous serum
title_full Analysis of cerebrospinal fluid from cattle with central nervous system disorders after storage for 24 hours with autologous serum
title_fullStr Analysis of cerebrospinal fluid from cattle with central nervous system disorders after storage for 24 hours with autologous serum
title_full_unstemmed Analysis of cerebrospinal fluid from cattle with central nervous system disorders after storage for 24 hours with autologous serum
title_short Analysis of cerebrospinal fluid from cattle with central nervous system disorders after storage for 24 hours with autologous serum
title_sort analysis of cerebrospinal fluid from cattle with central nervous system disorders after storage for 24 hours with autologous serum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542111/
https://www.ncbi.nlm.nih.gov/pubmed/26268228
http://dx.doi.org/10.1186/s12917-015-0502-x
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