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Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children
BACKGROUND: The most reliable indicator of Vitamin D status is circulating concentration of 25-hydroxycalciferol (25(OH) D) routinely determined by enzyme-immunoassays (EIA) methods. This study was performed to compare commonly used competitive protein-binding assays (CPBA)-based EIA with the gold s...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542329/ https://www.ncbi.nlm.nih.gov/pubmed/26330983 http://dx.doi.org/10.4103/2008-7802.161069 |
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author | Zahedi Rad, Maliheh Neyestani, Tirang Reza Nikooyeh, Bahareh Shariatzadeh, Nastaran Kalayi, Ali Khalaji, Niloufar Gharavi, Azam |
author_facet | Zahedi Rad, Maliheh Neyestani, Tirang Reza Nikooyeh, Bahareh Shariatzadeh, Nastaran Kalayi, Ali Khalaji, Niloufar Gharavi, Azam |
author_sort | Zahedi Rad, Maliheh |
collection | PubMed |
description | BACKGROUND: The most reliable indicator of Vitamin D status is circulating concentration of 25-hydroxycalciferol (25(OH) D) routinely determined by enzyme-immunoassays (EIA) methods. This study was performed to compare commonly used competitive protein-binding assays (CPBA)-based EIA with the gold standard, high-pressure liquid chromatography (HPLC). METHODS: Concentrations of 25(OH) D in sera from 257 randomly selected school children aged 9–11 years were determined by two methods of CPBA and HPLC. RESULTS: Mean 25(OH) D concentration was 22 ± 18.8 and 21.9 ± 15.6 nmol/L by CPBA and HPLC, respectively. However, mean 25(OH) D concentrations of the two methods became different after excluding undetectable samples (25.1 ± 18.9 vs. 29 ± 14.5 nmol/L, respectively; P = 0.04). Based on predefined Vitamin D deficiency as 25(OH) D < 12.5 nmol/L, CPBA sensitivity and specificity were 44.2% and 60.6%, respectively, compared to HPLC. In receiver operating characteristic curve analysis, the best cut-offs for CPBA was 5.8 nmol/L, which gave 82% sensitivity, but specificity was 17%. CONCLUSIONS: Though CPBA may be used as a screening tool, more reliable methods are needed for diagnostic purposes. |
format | Online Article Text |
id | pubmed-4542329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-45423292015-09-01 Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children Zahedi Rad, Maliheh Neyestani, Tirang Reza Nikooyeh, Bahareh Shariatzadeh, Nastaran Kalayi, Ali Khalaji, Niloufar Gharavi, Azam Int J Prev Med Original Article BACKGROUND: The most reliable indicator of Vitamin D status is circulating concentration of 25-hydroxycalciferol (25(OH) D) routinely determined by enzyme-immunoassays (EIA) methods. This study was performed to compare commonly used competitive protein-binding assays (CPBA)-based EIA with the gold standard, high-pressure liquid chromatography (HPLC). METHODS: Concentrations of 25(OH) D in sera from 257 randomly selected school children aged 9–11 years were determined by two methods of CPBA and HPLC. RESULTS: Mean 25(OH) D concentration was 22 ± 18.8 and 21.9 ± 15.6 nmol/L by CPBA and HPLC, respectively. However, mean 25(OH) D concentrations of the two methods became different after excluding undetectable samples (25.1 ± 18.9 vs. 29 ± 14.5 nmol/L, respectively; P = 0.04). Based on predefined Vitamin D deficiency as 25(OH) D < 12.5 nmol/L, CPBA sensitivity and specificity were 44.2% and 60.6%, respectively, compared to HPLC. In receiver operating characteristic curve analysis, the best cut-offs for CPBA was 5.8 nmol/L, which gave 82% sensitivity, but specificity was 17%. CONCLUSIONS: Though CPBA may be used as a screening tool, more reliable methods are needed for diagnostic purposes. Medknow Publications & Media Pvt Ltd 2015-07-27 /pmc/articles/PMC4542329/ /pubmed/26330983 http://dx.doi.org/10.4103/2008-7802.161069 Text en Copyright: © 2015 Zahedi Rad M. http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Original Article Zahedi Rad, Maliheh Neyestani, Tirang Reza Nikooyeh, Bahareh Shariatzadeh, Nastaran Kalayi, Ali Khalaji, Niloufar Gharavi, Azam Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children |
title | Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children |
title_full | Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children |
title_fullStr | Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children |
title_full_unstemmed | Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children |
title_short | Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children |
title_sort | competitive protein-binding assay-based enzyme-immunoassay method, compared to high-pressure liquid chromatography, has a very lower diagnostic value to detect vitamin d deficiency in 9–12 years children |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542329/ https://www.ncbi.nlm.nih.gov/pubmed/26330983 http://dx.doi.org/10.4103/2008-7802.161069 |
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