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Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children

BACKGROUND: The most reliable indicator of Vitamin D status is circulating concentration of 25-hydroxycalciferol (25(OH) D) routinely determined by enzyme-immunoassays (EIA) methods. This study was performed to compare commonly used competitive protein-binding assays (CPBA)-based EIA with the gold s...

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Autores principales: Zahedi Rad, Maliheh, Neyestani, Tirang Reza, Nikooyeh, Bahareh, Shariatzadeh, Nastaran, Kalayi, Ali, Khalaji, Niloufar, Gharavi, Azam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542329/
https://www.ncbi.nlm.nih.gov/pubmed/26330983
http://dx.doi.org/10.4103/2008-7802.161069
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author Zahedi Rad, Maliheh
Neyestani, Tirang Reza
Nikooyeh, Bahareh
Shariatzadeh, Nastaran
Kalayi, Ali
Khalaji, Niloufar
Gharavi, Azam
author_facet Zahedi Rad, Maliheh
Neyestani, Tirang Reza
Nikooyeh, Bahareh
Shariatzadeh, Nastaran
Kalayi, Ali
Khalaji, Niloufar
Gharavi, Azam
author_sort Zahedi Rad, Maliheh
collection PubMed
description BACKGROUND: The most reliable indicator of Vitamin D status is circulating concentration of 25-hydroxycalciferol (25(OH) D) routinely determined by enzyme-immunoassays (EIA) methods. This study was performed to compare commonly used competitive protein-binding assays (CPBA)-based EIA with the gold standard, high-pressure liquid chromatography (HPLC). METHODS: Concentrations of 25(OH) D in sera from 257 randomly selected school children aged 9–11 years were determined by two methods of CPBA and HPLC. RESULTS: Mean 25(OH) D concentration was 22 ± 18.8 and 21.9 ± 15.6 nmol/L by CPBA and HPLC, respectively. However, mean 25(OH) D concentrations of the two methods became different after excluding undetectable samples (25.1 ± 18.9 vs. 29 ± 14.5 nmol/L, respectively; P = 0.04). Based on predefined Vitamin D deficiency as 25(OH) D < 12.5 nmol/L, CPBA sensitivity and specificity were 44.2% and 60.6%, respectively, compared to HPLC. In receiver operating characteristic curve analysis, the best cut-offs for CPBA was 5.8 nmol/L, which gave 82% sensitivity, but specificity was 17%. CONCLUSIONS: Though CPBA may be used as a screening tool, more reliable methods are needed for diagnostic purposes.
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spelling pubmed-45423292015-09-01 Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children Zahedi Rad, Maliheh Neyestani, Tirang Reza Nikooyeh, Bahareh Shariatzadeh, Nastaran Kalayi, Ali Khalaji, Niloufar Gharavi, Azam Int J Prev Med Original Article BACKGROUND: The most reliable indicator of Vitamin D status is circulating concentration of 25-hydroxycalciferol (25(OH) D) routinely determined by enzyme-immunoassays (EIA) methods. This study was performed to compare commonly used competitive protein-binding assays (CPBA)-based EIA with the gold standard, high-pressure liquid chromatography (HPLC). METHODS: Concentrations of 25(OH) D in sera from 257 randomly selected school children aged 9–11 years were determined by two methods of CPBA and HPLC. RESULTS: Mean 25(OH) D concentration was 22 ± 18.8 and 21.9 ± 15.6 nmol/L by CPBA and HPLC, respectively. However, mean 25(OH) D concentrations of the two methods became different after excluding undetectable samples (25.1 ± 18.9 vs. 29 ± 14.5 nmol/L, respectively; P = 0.04). Based on predefined Vitamin D deficiency as 25(OH) D < 12.5 nmol/L, CPBA sensitivity and specificity were 44.2% and 60.6%, respectively, compared to HPLC. In receiver operating characteristic curve analysis, the best cut-offs for CPBA was 5.8 nmol/L, which gave 82% sensitivity, but specificity was 17%. CONCLUSIONS: Though CPBA may be used as a screening tool, more reliable methods are needed for diagnostic purposes. Medknow Publications & Media Pvt Ltd 2015-07-27 /pmc/articles/PMC4542329/ /pubmed/26330983 http://dx.doi.org/10.4103/2008-7802.161069 Text en Copyright: © 2015 Zahedi Rad M. http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Original Article
Zahedi Rad, Maliheh
Neyestani, Tirang Reza
Nikooyeh, Bahareh
Shariatzadeh, Nastaran
Kalayi, Ali
Khalaji, Niloufar
Gharavi, Azam
Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children
title Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children
title_full Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children
title_fullStr Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children
title_full_unstemmed Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children
title_short Competitive Protein-binding assay-based Enzyme-immunoassay Method, Compared to High-pressure Liquid Chromatography, Has a Very Lower Diagnostic Value to Detect Vitamin D Deficiency in 9–12 Years Children
title_sort competitive protein-binding assay-based enzyme-immunoassay method, compared to high-pressure liquid chromatography, has a very lower diagnostic value to detect vitamin d deficiency in 9–12 years children
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542329/
https://www.ncbi.nlm.nih.gov/pubmed/26330983
http://dx.doi.org/10.4103/2008-7802.161069
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