Cargando…

Maternal High-Fat Feeding Increases Placental Lipoprotein Lipase Activity by Reducing SIRT1 Expression in Mice

This study investigated how maternal overnutrition and obesity regulate expression and activation of proteins that facilitate lipid transport in the placenta. To create a maternal overnutrition and obesity model, primiparous C57BL/6 mice were fed a high-fat (HF) diet throughout gestation. Fetuses fr...

Descripción completa

Detalles Bibliográficos
Autores principales: Qiao, Liping, Guo, Zhuyu, Bosco, Chris, Guidotti, Stefano, Wang, Yunfeng, Wang, Mingyong, Parast, Mana, Schaack, Jerome, Hay, William W., Moore, Thomas R., Shao, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Diabetes Association 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542442/
https://www.ncbi.nlm.nih.gov/pubmed/25948680
http://dx.doi.org/10.2337/db14-1627
_version_ 1782386532442701824
author Qiao, Liping
Guo, Zhuyu
Bosco, Chris
Guidotti, Stefano
Wang, Yunfeng
Wang, Mingyong
Parast, Mana
Schaack, Jerome
Hay, William W.
Moore, Thomas R.
Shao, Jianhua
author_facet Qiao, Liping
Guo, Zhuyu
Bosco, Chris
Guidotti, Stefano
Wang, Yunfeng
Wang, Mingyong
Parast, Mana
Schaack, Jerome
Hay, William W.
Moore, Thomas R.
Shao, Jianhua
author_sort Qiao, Liping
collection PubMed
description This study investigated how maternal overnutrition and obesity regulate expression and activation of proteins that facilitate lipid transport in the placenta. To create a maternal overnutrition and obesity model, primiparous C57BL/6 mice were fed a high-fat (HF) diet throughout gestation. Fetuses from HF-fed dams had significantly increased serum levels of free fatty acid and body fat. Despite no significant difference in placental weight, lipoprotein lipase (LPL) protein levels and activity were remarkably elevated in placentas from HF-fed dams. Increased triglyceride content and mRNA levels of CD36, VLDLr, FABP3, FABPpm, and GPAT2 and -3 were also found in placentas from HF-fed dams. Although both peroxisome proliferator–activated receptor-γ (PPARγ) and CCAAT/enhancer binding protein-α protein levels were significantly increased in placentas of the HF group, only PPARγ exhibited a stimulative effect on LPL expression in cultured JEG-3 human trophoblasts. Maternal HF feeding remarkably decreased SIRT1 expression in placentas. Through use of an SIRT1 activator and inhibitor and cultured trophoblasts, an inhibitory effect of SIRT1 on LPL expression was demonstrated. We also found that SIRT1 suppresses PPARγ expression in trophoblasts. Most importantly, inhibition of PPARγ abolished the SIRT1-mediated regulatory effect on LPL expression. Together, these results indicate that maternal overnutrition induces LPL expression in trophoblasts by reducing the inhibitory effect of SIRT1 on PPARγ.
format Online
Article
Text
id pubmed-4542442
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher American Diabetes Association
record_format MEDLINE/PubMed
spelling pubmed-45424422016-09-01 Maternal High-Fat Feeding Increases Placental Lipoprotein Lipase Activity by Reducing SIRT1 Expression in Mice Qiao, Liping Guo, Zhuyu Bosco, Chris Guidotti, Stefano Wang, Yunfeng Wang, Mingyong Parast, Mana Schaack, Jerome Hay, William W. Moore, Thomas R. Shao, Jianhua Diabetes Obesity Studies This study investigated how maternal overnutrition and obesity regulate expression and activation of proteins that facilitate lipid transport in the placenta. To create a maternal overnutrition and obesity model, primiparous C57BL/6 mice were fed a high-fat (HF) diet throughout gestation. Fetuses from HF-fed dams had significantly increased serum levels of free fatty acid and body fat. Despite no significant difference in placental weight, lipoprotein lipase (LPL) protein levels and activity were remarkably elevated in placentas from HF-fed dams. Increased triglyceride content and mRNA levels of CD36, VLDLr, FABP3, FABPpm, and GPAT2 and -3 were also found in placentas from HF-fed dams. Although both peroxisome proliferator–activated receptor-γ (PPARγ) and CCAAT/enhancer binding protein-α protein levels were significantly increased in placentas of the HF group, only PPARγ exhibited a stimulative effect on LPL expression in cultured JEG-3 human trophoblasts. Maternal HF feeding remarkably decreased SIRT1 expression in placentas. Through use of an SIRT1 activator and inhibitor and cultured trophoblasts, an inhibitory effect of SIRT1 on LPL expression was demonstrated. We also found that SIRT1 suppresses PPARγ expression in trophoblasts. Most importantly, inhibition of PPARγ abolished the SIRT1-mediated regulatory effect on LPL expression. Together, these results indicate that maternal overnutrition induces LPL expression in trophoblasts by reducing the inhibitory effect of SIRT1 on PPARγ. American Diabetes Association 2015-09 2015-05-06 /pmc/articles/PMC4542442/ /pubmed/25948680 http://dx.doi.org/10.2337/db14-1627 Text en © 2015 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.
spellingShingle Obesity Studies
Qiao, Liping
Guo, Zhuyu
Bosco, Chris
Guidotti, Stefano
Wang, Yunfeng
Wang, Mingyong
Parast, Mana
Schaack, Jerome
Hay, William W.
Moore, Thomas R.
Shao, Jianhua
Maternal High-Fat Feeding Increases Placental Lipoprotein Lipase Activity by Reducing SIRT1 Expression in Mice
title Maternal High-Fat Feeding Increases Placental Lipoprotein Lipase Activity by Reducing SIRT1 Expression in Mice
title_full Maternal High-Fat Feeding Increases Placental Lipoprotein Lipase Activity by Reducing SIRT1 Expression in Mice
title_fullStr Maternal High-Fat Feeding Increases Placental Lipoprotein Lipase Activity by Reducing SIRT1 Expression in Mice
title_full_unstemmed Maternal High-Fat Feeding Increases Placental Lipoprotein Lipase Activity by Reducing SIRT1 Expression in Mice
title_short Maternal High-Fat Feeding Increases Placental Lipoprotein Lipase Activity by Reducing SIRT1 Expression in Mice
title_sort maternal high-fat feeding increases placental lipoprotein lipase activity by reducing sirt1 expression in mice
topic Obesity Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542442/
https://www.ncbi.nlm.nih.gov/pubmed/25948680
http://dx.doi.org/10.2337/db14-1627
work_keys_str_mv AT qiaoliping maternalhighfatfeedingincreasesplacentallipoproteinlipaseactivitybyreducingsirt1expressioninmice
AT guozhuyu maternalhighfatfeedingincreasesplacentallipoproteinlipaseactivitybyreducingsirt1expressioninmice
AT boscochris maternalhighfatfeedingincreasesplacentallipoproteinlipaseactivitybyreducingsirt1expressioninmice
AT guidottistefano maternalhighfatfeedingincreasesplacentallipoproteinlipaseactivitybyreducingsirt1expressioninmice
AT wangyunfeng maternalhighfatfeedingincreasesplacentallipoproteinlipaseactivitybyreducingsirt1expressioninmice
AT wangmingyong maternalhighfatfeedingincreasesplacentallipoproteinlipaseactivitybyreducingsirt1expressioninmice
AT parastmana maternalhighfatfeedingincreasesplacentallipoproteinlipaseactivitybyreducingsirt1expressioninmice
AT schaackjerome maternalhighfatfeedingincreasesplacentallipoproteinlipaseactivitybyreducingsirt1expressioninmice
AT haywilliamw maternalhighfatfeedingincreasesplacentallipoproteinlipaseactivitybyreducingsirt1expressioninmice
AT moorethomasr maternalhighfatfeedingincreasesplacentallipoproteinlipaseactivitybyreducingsirt1expressioninmice
AT shaojianhua maternalhighfatfeedingincreasesplacentallipoproteinlipaseactivitybyreducingsirt1expressioninmice