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RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress
The response regulator OtpR is critical for the growth, morphology and virulence of Brucella melitensis. Compared to its wild type strain 16 M, B. melitensis 16 MΔotpR mutant has decreased tolerance to acid stress. To analyze the genes regulated by OtpR under acid stress, we performed RNA-seq whole...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542472/ https://www.ncbi.nlm.nih.gov/pubmed/26242322 http://dx.doi.org/10.1038/srep10864 |
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author | Liu, Wenxiao Dong, Hao Li, Jing Ou, Qixing Lv, Yujin Wang, Xiaolei Xiang, Zuoshuang He, Yongqun Wu, Qingmin |
author_facet | Liu, Wenxiao Dong, Hao Li, Jing Ou, Qixing Lv, Yujin Wang, Xiaolei Xiang, Zuoshuang He, Yongqun Wu, Qingmin |
author_sort | Liu, Wenxiao |
collection | PubMed |
description | The response regulator OtpR is critical for the growth, morphology and virulence of Brucella melitensis. Compared to its wild type strain 16 M, B. melitensis 16 MΔotpR mutant has decreased tolerance to acid stress. To analyze the genes regulated by OtpR under acid stress, we performed RNA-seq whole transcriptome analysis of 16 MΔotpR and 16 M. In total, 501 differentially expressed genes were identified, including 390 down-regulated and 111 up-regulated genes. Among these genes, 209 were associated with bacterial metabolism, including 54 genes involving carbohydrate metabolism, 13 genes associated with nitrogen metabolism, and seven genes associated with iron metabolism. The 16 MΔotpR also decreased capacity to utilize different carbon sources and to tolerate iron limitation in culture experiments. Notably, OtpR regulated many Brucella virulence factors essential for B. melitensis intracellular survival. For instance, the virB operon encoding type IV secretion system was significantly down-regulated, and 36 known transcriptional regulators (e.g., vjbR and blxR) were differentially expressed in 16 MΔotpR. Selected RNA-seq results were experimentally confirmed by RT-PCR and RT-qPCR. Overall, these results deciphered differential phenomena associated with virulence, environmental stresses and cell morphology in 16 MΔotpR and 16 M, which provided important information for understanding the detailed OtpR-regulated interaction networks and Brucella pathogenesis. |
format | Online Article Text |
id | pubmed-4542472 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-45424722015-09-01 RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress Liu, Wenxiao Dong, Hao Li, Jing Ou, Qixing Lv, Yujin Wang, Xiaolei Xiang, Zuoshuang He, Yongqun Wu, Qingmin Sci Rep Article The response regulator OtpR is critical for the growth, morphology and virulence of Brucella melitensis. Compared to its wild type strain 16 M, B. melitensis 16 MΔotpR mutant has decreased tolerance to acid stress. To analyze the genes regulated by OtpR under acid stress, we performed RNA-seq whole transcriptome analysis of 16 MΔotpR and 16 M. In total, 501 differentially expressed genes were identified, including 390 down-regulated and 111 up-regulated genes. Among these genes, 209 were associated with bacterial metabolism, including 54 genes involving carbohydrate metabolism, 13 genes associated with nitrogen metabolism, and seven genes associated with iron metabolism. The 16 MΔotpR also decreased capacity to utilize different carbon sources and to tolerate iron limitation in culture experiments. Notably, OtpR regulated many Brucella virulence factors essential for B. melitensis intracellular survival. For instance, the virB operon encoding type IV secretion system was significantly down-regulated, and 36 known transcriptional regulators (e.g., vjbR and blxR) were differentially expressed in 16 MΔotpR. Selected RNA-seq results were experimentally confirmed by RT-PCR and RT-qPCR. Overall, these results deciphered differential phenomena associated with virulence, environmental stresses and cell morphology in 16 MΔotpR and 16 M, which provided important information for understanding the detailed OtpR-regulated interaction networks and Brucella pathogenesis. Nature Publishing Group 2015-08-05 /pmc/articles/PMC4542472/ /pubmed/26242322 http://dx.doi.org/10.1038/srep10864 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Liu, Wenxiao Dong, Hao Li, Jing Ou, Qixing Lv, Yujin Wang, Xiaolei Xiang, Zuoshuang He, Yongqun Wu, Qingmin RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress |
title | RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress |
title_full | RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress |
title_fullStr | RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress |
title_full_unstemmed | RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress |
title_short | RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress |
title_sort | rna-seq reveals the critical role of otpr in regulating brucella melitensis metabolism and virulence under acidic stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542472/ https://www.ncbi.nlm.nih.gov/pubmed/26242322 http://dx.doi.org/10.1038/srep10864 |
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