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TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration

Recent studies suggest that cell therapy may be an effective way to repair intervertebral disc degeneration. As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively. The objective of this study was to explore the effects of TGF-β1 during bone marrow mesenchym...

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Autores principales: Yang, Huilin, Cao, Cheng, Wu, Chunshen, Yuan, Chenxi, Gu, Qiaoli, Shi, Qing, Zou, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542522/
https://www.ncbi.nlm.nih.gov/pubmed/26289964
http://dx.doi.org/10.1038/srep13254
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author Yang, Huilin
Cao, Cheng
Wu, Chunshen
Yuan, Chenxi
Gu, Qiaoli
Shi, Qing
Zou, Jun
author_facet Yang, Huilin
Cao, Cheng
Wu, Chunshen
Yuan, Chenxi
Gu, Qiaoli
Shi, Qing
Zou, Jun
author_sort Yang, Huilin
collection PubMed
description Recent studies suggest that cell therapy may be an effective way to repair intervertebral disc degeneration. As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively. The objective of this study was to explore the effects of TGF-β1 during bone marrow mesenchymal stem cells-based therapy for disc degeneration. In vitro assays demonstrated that co-culturing of nucleus pulposus cells with bone marrow mesenchymal stem cells resulted in significantly higher levels of TGF-βl secretion. This increase inhibited IκB phosphorylation and NF-κB activation, detected by western blot analysis. Meanwhile, in a rabbit model, MRI analysis revealed significant recovery of signal intensity in the degenerative discs of rabbits receiving cells transplantation, than receiving cells treated with a TGF-β1 inhibitor or saline. These findings indicated that enhanced TGF-β1 production recovered the degeneration of intervertebral disc. And also immunohistochemical staining detected enhanced collagen II expression in the rabbits treated with cell transplantation. However, the NF-κB positive cells were significantly less than other two control groups. Thus, cell therapy promoted TGF-β1 expression in nucleus pulposus, leading to anti-inflammatory effects via the inhibition of NF-κB, and the amelioration of disc degradation due to increased expression of collagen II and aggrecan in degenerative intervertebral disc.
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spelling pubmed-45425222015-09-01 TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration Yang, Huilin Cao, Cheng Wu, Chunshen Yuan, Chenxi Gu, Qiaoli Shi, Qing Zou, Jun Sci Rep Article Recent studies suggest that cell therapy may be an effective way to repair intervertebral disc degeneration. As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively. The objective of this study was to explore the effects of TGF-β1 during bone marrow mesenchymal stem cells-based therapy for disc degeneration. In vitro assays demonstrated that co-culturing of nucleus pulposus cells with bone marrow mesenchymal stem cells resulted in significantly higher levels of TGF-βl secretion. This increase inhibited IκB phosphorylation and NF-κB activation, detected by western blot analysis. Meanwhile, in a rabbit model, MRI analysis revealed significant recovery of signal intensity in the degenerative discs of rabbits receiving cells transplantation, than receiving cells treated with a TGF-β1 inhibitor or saline. These findings indicated that enhanced TGF-β1 production recovered the degeneration of intervertebral disc. And also immunohistochemical staining detected enhanced collagen II expression in the rabbits treated with cell transplantation. However, the NF-κB positive cells were significantly less than other two control groups. Thus, cell therapy promoted TGF-β1 expression in nucleus pulposus, leading to anti-inflammatory effects via the inhibition of NF-κB, and the amelioration of disc degradation due to increased expression of collagen II and aggrecan in degenerative intervertebral disc. Nature Publishing Group 2015-08-20 /pmc/articles/PMC4542522/ /pubmed/26289964 http://dx.doi.org/10.1038/srep13254 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Yang, Huilin
Cao, Cheng
Wu, Chunshen
Yuan, Chenxi
Gu, Qiaoli
Shi, Qing
Zou, Jun
TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration
title TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration
title_full TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration
title_fullStr TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration
title_full_unstemmed TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration
title_short TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration
title_sort tgf-βl suppresses inflammation in cell therapy for intervertebral disc degeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542522/
https://www.ncbi.nlm.nih.gov/pubmed/26289964
http://dx.doi.org/10.1038/srep13254
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