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Coagulation of β-conglycinin, glycinin and isoflavones induced by calcium chloride in soymilk

The coagulation of β-conglycinin (7S), glycinin (11S) and isoflavones induced by calcium chloride was investigated. Approximately 92.6% of the soymilk proteins were coagulated into the soymilk pellet fraction (SPF) after the addition of 5 mM calcium chloride. SDS-PAGE and two-dimensional electrophor...

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Autores principales: Hsiao, Yu-Hsuan, Yu, Chia-Jung, Li, Wen-Tai, Hsieh, Jung-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542527/
https://www.ncbi.nlm.nih.gov/pubmed/26260443
http://dx.doi.org/10.1038/srep13018
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author Hsiao, Yu-Hsuan
Yu, Chia-Jung
Li, Wen-Tai
Hsieh, Jung-Feng
author_facet Hsiao, Yu-Hsuan
Yu, Chia-Jung
Li, Wen-Tai
Hsieh, Jung-Feng
author_sort Hsiao, Yu-Hsuan
collection PubMed
description The coagulation of β-conglycinin (7S), glycinin (11S) and isoflavones induced by calcium chloride was investigated. Approximately 92.6% of the soymilk proteins were coagulated into the soymilk pellet fraction (SPF) after the addition of 5 mM calcium chloride. SDS-PAGE and two-dimensional electrophoresis analysis indicated that most of the 7S (α’, α and β), 11S acidic (A1a, A1b, A2, A3 and A4) and 11S basic (B1a) proteins in the SSF were coagulated into the SPF after treatment with 5 mM calcium chloride. Isoflavones, including daidzein and genistein, were also coagulated into the SPF after the addition of 5 mM calcium chloride. The amounts of daidzein and genistein in the SSF decreased to 39.4 ± 1.6 and 11.8 ± 7.0%, respectively. HPLC analysis suggested that daidzein and genistein were bound with 7S and 11S proteins and then were coprecipitated into the SPF by 5 mM calcium chloride.
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spelling pubmed-45425272015-09-01 Coagulation of β-conglycinin, glycinin and isoflavones induced by calcium chloride in soymilk Hsiao, Yu-Hsuan Yu, Chia-Jung Li, Wen-Tai Hsieh, Jung-Feng Sci Rep Article The coagulation of β-conglycinin (7S), glycinin (11S) and isoflavones induced by calcium chloride was investigated. Approximately 92.6% of the soymilk proteins were coagulated into the soymilk pellet fraction (SPF) after the addition of 5 mM calcium chloride. SDS-PAGE and two-dimensional electrophoresis analysis indicated that most of the 7S (α’, α and β), 11S acidic (A1a, A1b, A2, A3 and A4) and 11S basic (B1a) proteins in the SSF were coagulated into the SPF after treatment with 5 mM calcium chloride. Isoflavones, including daidzein and genistein, were also coagulated into the SPF after the addition of 5 mM calcium chloride. The amounts of daidzein and genistein in the SSF decreased to 39.4 ± 1.6 and 11.8 ± 7.0%, respectively. HPLC analysis suggested that daidzein and genistein were bound with 7S and 11S proteins and then were coprecipitated into the SPF by 5 mM calcium chloride. Nature Publishing Group 2015-08-11 /pmc/articles/PMC4542527/ /pubmed/26260443 http://dx.doi.org/10.1038/srep13018 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Hsiao, Yu-Hsuan
Yu, Chia-Jung
Li, Wen-Tai
Hsieh, Jung-Feng
Coagulation of β-conglycinin, glycinin and isoflavones induced by calcium chloride in soymilk
title Coagulation of β-conglycinin, glycinin and isoflavones induced by calcium chloride in soymilk
title_full Coagulation of β-conglycinin, glycinin and isoflavones induced by calcium chloride in soymilk
title_fullStr Coagulation of β-conglycinin, glycinin and isoflavones induced by calcium chloride in soymilk
title_full_unstemmed Coagulation of β-conglycinin, glycinin and isoflavones induced by calcium chloride in soymilk
title_short Coagulation of β-conglycinin, glycinin and isoflavones induced by calcium chloride in soymilk
title_sort coagulation of β-conglycinin, glycinin and isoflavones induced by calcium chloride in soymilk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542527/
https://www.ncbi.nlm.nih.gov/pubmed/26260443
http://dx.doi.org/10.1038/srep13018
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