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Advanced backcross QTL analysis reveals complicated genetic control of rice grain shape in a japonica × indica cross
Grain shape is an important trait for improving rice yield. A number of quantitative trait loci (QTLs) for this trait have been identified by using primary F(2) mapping populations and recombinant inbred lines, in which QTLs with a small effect are harder to detect than they would be in advanced gen...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society of Breeding
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542931/ https://www.ncbi.nlm.nih.gov/pubmed/26366113 http://dx.doi.org/10.1270/jsbbs.65.308 |
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author | Nagata, Kazufumi Ando, Tsuyu Nonoue, Yasunori Mizubayashi, Tatsumi Kitazawa, Noriyuki Shomura, Ayahiko Matsubara, Kazuki Ono, Nozomi Mizobuchi, Ritsuko Shibaya, Taeko Ogiso-Tanaka, Eri Hori, Kiyosumi Yano, Masahiro Fukuoka, Shuichi |
author_facet | Nagata, Kazufumi Ando, Tsuyu Nonoue, Yasunori Mizubayashi, Tatsumi Kitazawa, Noriyuki Shomura, Ayahiko Matsubara, Kazuki Ono, Nozomi Mizobuchi, Ritsuko Shibaya, Taeko Ogiso-Tanaka, Eri Hori, Kiyosumi Yano, Masahiro Fukuoka, Shuichi |
author_sort | Nagata, Kazufumi |
collection | PubMed |
description | Grain shape is an important trait for improving rice yield. A number of quantitative trait loci (QTLs) for this trait have been identified by using primary F(2) mapping populations and recombinant inbred lines, in which QTLs with a small effect are harder to detect than they would be in advanced generations. In this study, we developed two advanced mapping populations (chromosome segment substitution lines [CSSLs] and BC(4)F(2) lines consisting of more than 2000 individuals) in the genetic backgrounds of two improved cultivars: a japonica cultivar (Koshihikari) with short, round grains, and an indica cultivar (IR64) with long, slender grains. We compared the ability of these materials to reveal QTLs for grain shape with that of an F(2) population. Only 8 QTLs for grain length or grain width were detected in the F(2) population, versus 47 in the CSSL population and 65 in the BC(4)F(2) population. These results strongly suggest that advanced mapping populations can reveal QTLs for agronomic traits under complicated genetic control, and that DNA markers linked with the QTLs are useful for choosing superior allelic combinations to enhance grain shape in the Koshihikari and IR64 genetic backgrounds. |
format | Online Article Text |
id | pubmed-4542931 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Japanese Society of Breeding |
record_format | MEDLINE/PubMed |
spelling | pubmed-45429312015-09-11 Advanced backcross QTL analysis reveals complicated genetic control of rice grain shape in a japonica × indica cross Nagata, Kazufumi Ando, Tsuyu Nonoue, Yasunori Mizubayashi, Tatsumi Kitazawa, Noriyuki Shomura, Ayahiko Matsubara, Kazuki Ono, Nozomi Mizobuchi, Ritsuko Shibaya, Taeko Ogiso-Tanaka, Eri Hori, Kiyosumi Yano, Masahiro Fukuoka, Shuichi Breed Sci Research Paper Grain shape is an important trait for improving rice yield. A number of quantitative trait loci (QTLs) for this trait have been identified by using primary F(2) mapping populations and recombinant inbred lines, in which QTLs with a small effect are harder to detect than they would be in advanced generations. In this study, we developed two advanced mapping populations (chromosome segment substitution lines [CSSLs] and BC(4)F(2) lines consisting of more than 2000 individuals) in the genetic backgrounds of two improved cultivars: a japonica cultivar (Koshihikari) with short, round grains, and an indica cultivar (IR64) with long, slender grains. We compared the ability of these materials to reveal QTLs for grain shape with that of an F(2) population. Only 8 QTLs for grain length or grain width were detected in the F(2) population, versus 47 in the CSSL population and 65 in the BC(4)F(2) population. These results strongly suggest that advanced mapping populations can reveal QTLs for agronomic traits under complicated genetic control, and that DNA markers linked with the QTLs are useful for choosing superior allelic combinations to enhance grain shape in the Koshihikari and IR64 genetic backgrounds. Japanese Society of Breeding 2015-09 2015-09-01 /pmc/articles/PMC4542931/ /pubmed/26366113 http://dx.doi.org/10.1270/jsbbs.65.308 Text en Copyright © 2015 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Nagata, Kazufumi Ando, Tsuyu Nonoue, Yasunori Mizubayashi, Tatsumi Kitazawa, Noriyuki Shomura, Ayahiko Matsubara, Kazuki Ono, Nozomi Mizobuchi, Ritsuko Shibaya, Taeko Ogiso-Tanaka, Eri Hori, Kiyosumi Yano, Masahiro Fukuoka, Shuichi Advanced backcross QTL analysis reveals complicated genetic control of rice grain shape in a japonica × indica cross |
title | Advanced backcross QTL analysis reveals complicated genetic control of rice grain shape in a japonica × indica cross |
title_full | Advanced backcross QTL analysis reveals complicated genetic control of rice grain shape in a japonica × indica cross |
title_fullStr | Advanced backcross QTL analysis reveals complicated genetic control of rice grain shape in a japonica × indica cross |
title_full_unstemmed | Advanced backcross QTL analysis reveals complicated genetic control of rice grain shape in a japonica × indica cross |
title_short | Advanced backcross QTL analysis reveals complicated genetic control of rice grain shape in a japonica × indica cross |
title_sort | advanced backcross qtl analysis reveals complicated genetic control of rice grain shape in a japonica × indica cross |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542931/ https://www.ncbi.nlm.nih.gov/pubmed/26366113 http://dx.doi.org/10.1270/jsbbs.65.308 |
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