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Microsatellite markers for studies with the carnivorous plant Philcoxia minensis (Plantaginaceae)(1)
PREMISE OF THE STUDY: Microsatellite markers were developed for the critically endangered carnivorous species Philcoxia minensis (Plantaginaceae) for further population genetic studies aiming at its conservation. METHODS AND RESULTS: We identified 29 clones containing 40 microsatellites from a genom...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542941/ https://www.ncbi.nlm.nih.gov/pubmed/26312194 http://dx.doi.org/10.3732/apps.1500035 |
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author | Scatigna, André V. Oliveira, Fernanda A. Mantello, Camila C. Francisco, Patrícia M. Souza, Anete P. Simões, André O. |
author_facet | Scatigna, André V. Oliveira, Fernanda A. Mantello, Camila C. Francisco, Patrícia M. Souza, Anete P. Simões, André O. |
author_sort | Scatigna, André V. |
collection | PubMed |
description | PREMISE OF THE STUDY: Microsatellite markers were developed for the critically endangered carnivorous species Philcoxia minensis (Plantaginaceae) for further population genetic studies aiming at its conservation. METHODS AND RESULTS: We identified 29 clones containing 40 microsatellites from a genomic enriched library. A total of 27 primer pairs were developed and evaluated in 30 individuals of a natural P. minensis population. Seventeen markers successfully presented amplification products within the expected size range, of which 12 were polymorphic. The expected and observed heterozygosities ranged from 0.03 to 0.65 and from 0.00 to 0.77, respectively. Positive transferability with the related species P. bahiensis was observed for the same 17 markers. CONCLUSIONS: The 12 polymorphic microsatellite markers are suitable for studies in genetic diversity and structure, mating system, and gene flow in P. minensis and also may be useful for similar issues regarding the related species P. bahiensis. |
format | Online Article Text |
id | pubmed-4542941 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-45429412015-08-26 Microsatellite markers for studies with the carnivorous plant Philcoxia minensis (Plantaginaceae)(1) Scatigna, André V. Oliveira, Fernanda A. Mantello, Camila C. Francisco, Patrícia M. Souza, Anete P. Simões, André O. Appl Plant Sci Primer Note PREMISE OF THE STUDY: Microsatellite markers were developed for the critically endangered carnivorous species Philcoxia minensis (Plantaginaceae) for further population genetic studies aiming at its conservation. METHODS AND RESULTS: We identified 29 clones containing 40 microsatellites from a genomic enriched library. A total of 27 primer pairs were developed and evaluated in 30 individuals of a natural P. minensis population. Seventeen markers successfully presented amplification products within the expected size range, of which 12 were polymorphic. The expected and observed heterozygosities ranged from 0.03 to 0.65 and from 0.00 to 0.77, respectively. Positive transferability with the related species P. bahiensis was observed for the same 17 markers. CONCLUSIONS: The 12 polymorphic microsatellite markers are suitable for studies in genetic diversity and structure, mating system, and gene flow in P. minensis and also may be useful for similar issues regarding the related species P. bahiensis. Botanical Society of America 2015-08-17 /pmc/articles/PMC4542941/ /pubmed/26312194 http://dx.doi.org/10.3732/apps.1500035 Text en © 2015 Scatigna et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Scatigna, André V. Oliveira, Fernanda A. Mantello, Camila C. Francisco, Patrícia M. Souza, Anete P. Simões, André O. Microsatellite markers for studies with the carnivorous plant Philcoxia minensis (Plantaginaceae)(1) |
title | Microsatellite markers for studies with the carnivorous plant Philcoxia minensis (Plantaginaceae)(1) |
title_full | Microsatellite markers for studies with the carnivorous plant Philcoxia minensis (Plantaginaceae)(1) |
title_fullStr | Microsatellite markers for studies with the carnivorous plant Philcoxia minensis (Plantaginaceae)(1) |
title_full_unstemmed | Microsatellite markers for studies with the carnivorous plant Philcoxia minensis (Plantaginaceae)(1) |
title_short | Microsatellite markers for studies with the carnivorous plant Philcoxia minensis (Plantaginaceae)(1) |
title_sort | microsatellite markers for studies with the carnivorous plant philcoxia minensis (plantaginaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542941/ https://www.ncbi.nlm.nih.gov/pubmed/26312194 http://dx.doi.org/10.3732/apps.1500035 |
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