Development and characterization of polymorphic microsatellite loci for Saxifraga egregia (Saxifragaceae)(1)

PREMISE OF THE STUDY: Saxifraga egregia (Saxifragaceae) is a perennial herb that is endemic to the Qinghai–Tibet Plateau. We developed 12 polymorphic microsatellite loci for S. egregia to investigate its population genetics. METHODS AND RESULTS: Forty-eight pairs of microsatellite primers (including...

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Detalles Bibliográficos
Autores principales: Zhang, Faqi, Li, Yinhu, Gao, Qingbo, Lei, Shuyun, Khan, Gulzar, Yang, Huiling, Chen, Shilong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542942/
https://www.ncbi.nlm.nih.gov/pubmed/26312195
http://dx.doi.org/10.3732/apps.1500037
Descripción
Sumario:PREMISE OF THE STUDY: Saxifraga egregia (Saxifragaceae) is a perennial herb that is endemic to the Qinghai–Tibet Plateau. We developed 12 polymorphic microsatellite loci for S. egregia to investigate its population genetics. METHODS AND RESULTS: Forty-eight pairs of microsatellite primers (including 36 monomorphic loci) were isolated and characterized by magnetic bead enrichment. Twelve of these markers showed polymorphism, and the number of alleles per locus ranged from four to 14 across 50 individuals from three populations of S. egregia. No linkage disequilibrium was detected in any pair of loci. CONCLUSIONS: These polymorphic markers are expected to be helpful in further studies on the systematics and phylogeography of S. egregia in the Qinghai–Tibet Plateau.

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