Development and characterization of polymorphic microsatellite loci for Saxifraga egregia (Saxifragaceae)(1)

PREMISE OF THE STUDY: Saxifraga egregia (Saxifragaceae) is a perennial herb that is endemic to the Qinghai–Tibet Plateau. We developed 12 polymorphic microsatellite loci for S. egregia to investigate its population genetics. METHODS AND RESULTS: Forty-eight pairs of microsatellite primers (including...

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Detalles Bibliográficos
Autores principales: Zhang, Faqi, Li, Yinhu, Gao, Qingbo, Lei, Shuyun, Khan, Gulzar, Yang, Huiling, Chen, Shilong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542942/
https://www.ncbi.nlm.nih.gov/pubmed/26312195
http://dx.doi.org/10.3732/apps.1500037
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author Zhang, Faqi
Li, Yinhu
Gao, Qingbo
Lei, Shuyun
Khan, Gulzar
Yang, Huiling
Chen, Shilong
author_facet Zhang, Faqi
Li, Yinhu
Gao, Qingbo
Lei, Shuyun
Khan, Gulzar
Yang, Huiling
Chen, Shilong
author_sort Zhang, Faqi
collection PubMed
description PREMISE OF THE STUDY: Saxifraga egregia (Saxifragaceae) is a perennial herb that is endemic to the Qinghai–Tibet Plateau. We developed 12 polymorphic microsatellite loci for S. egregia to investigate its population genetics. METHODS AND RESULTS: Forty-eight pairs of microsatellite primers (including 36 monomorphic loci) were isolated and characterized by magnetic bead enrichment. Twelve of these markers showed polymorphism, and the number of alleles per locus ranged from four to 14 across 50 individuals from three populations of S. egregia. No linkage disequilibrium was detected in any pair of loci. CONCLUSIONS: These polymorphic markers are expected to be helpful in further studies on the systematics and phylogeography of S. egregia in the Qinghai–Tibet Plateau.
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spelling pubmed-45429422015-08-26 Development and characterization of polymorphic microsatellite loci for Saxifraga egregia (Saxifragaceae)(1) Zhang, Faqi Li, Yinhu Gao, Qingbo Lei, Shuyun Khan, Gulzar Yang, Huiling Chen, Shilong Appl Plant Sci Primer Note PREMISE OF THE STUDY: Saxifraga egregia (Saxifragaceae) is a perennial herb that is endemic to the Qinghai–Tibet Plateau. We developed 12 polymorphic microsatellite loci for S. egregia to investigate its population genetics. METHODS AND RESULTS: Forty-eight pairs of microsatellite primers (including 36 monomorphic loci) were isolated and characterized by magnetic bead enrichment. Twelve of these markers showed polymorphism, and the number of alleles per locus ranged from four to 14 across 50 individuals from three populations of S. egregia. No linkage disequilibrium was detected in any pair of loci. CONCLUSIONS: These polymorphic markers are expected to be helpful in further studies on the systematics and phylogeography of S. egregia in the Qinghai–Tibet Plateau. Botanical Society of America 2015-08-10 /pmc/articles/PMC4542942/ /pubmed/26312195 http://dx.doi.org/10.3732/apps.1500037 Text en © 2015 Zhang et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Zhang, Faqi
Li, Yinhu
Gao, Qingbo
Lei, Shuyun
Khan, Gulzar
Yang, Huiling
Chen, Shilong
Development and characterization of polymorphic microsatellite loci for Saxifraga egregia (Saxifragaceae)(1)
title Development and characterization of polymorphic microsatellite loci for Saxifraga egregia (Saxifragaceae)(1)
title_full Development and characterization of polymorphic microsatellite loci for Saxifraga egregia (Saxifragaceae)(1)
title_fullStr Development and characterization of polymorphic microsatellite loci for Saxifraga egregia (Saxifragaceae)(1)
title_full_unstemmed Development and characterization of polymorphic microsatellite loci for Saxifraga egregia (Saxifragaceae)(1)
title_short Development and characterization of polymorphic microsatellite loci for Saxifraga egregia (Saxifragaceae)(1)
title_sort development and characterization of polymorphic microsatellite loci for saxifraga egregia (saxifragaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542942/
https://www.ncbi.nlm.nih.gov/pubmed/26312195
http://dx.doi.org/10.3732/apps.1500037
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