Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae)(1)

PREMISE OF THE STUDY: Microsatellite markers were developed for Callitris sulcata (Cupressaceae), an endangered conifer species in New Caledonia. METHODS AND RESULTS: Using sequencing by synthesis (SBS) of an RNA-Seq library, 15 polymorphic nuclear and chloroplast microsatellite markers were develop...

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Autores principales: Sakaguchi, Shota, Lannuzel, Guillaume, Fogliani, Bruno, Wulff, Adrien S., L’Huillier, Laurent, Kurata, Seikan, Ueno, Saneyoshi, Isagi, Yuji, Tsumura, Yoshihiko, Ito, Motomi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542945/
https://www.ncbi.nlm.nih.gov/pubmed/26312198
http://dx.doi.org/10.3732/apps.1500045
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author Sakaguchi, Shota
Lannuzel, Guillaume
Fogliani, Bruno
Wulff, Adrien S.
L’Huillier, Laurent
Kurata, Seikan
Ueno, Saneyoshi
Isagi, Yuji
Tsumura, Yoshihiko
Ito, Motomi
author_facet Sakaguchi, Shota
Lannuzel, Guillaume
Fogliani, Bruno
Wulff, Adrien S.
L’Huillier, Laurent
Kurata, Seikan
Ueno, Saneyoshi
Isagi, Yuji
Tsumura, Yoshihiko
Ito, Motomi
author_sort Sakaguchi, Shota
collection PubMed
description PREMISE OF THE STUDY: Microsatellite markers were developed for Callitris sulcata (Cupressaceae), an endangered conifer species in New Caledonia. METHODS AND RESULTS: Using sequencing by synthesis (SBS) of an RNA-Seq library, 15 polymorphic nuclear and chloroplast microsatellite markers were developed. When evaluated with 48 individuals, these markers showed genetic variations ranging from two to 15 alleles and expected heterozygosity ranging from 0 to 0.881. CONCLUSIONS: These markers will be useful for examining the genetic diversity and structure of remaining wild populations and improving the genetic status of ex situ populations.
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spelling pubmed-45429452015-08-26 Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae)(1) Sakaguchi, Shota Lannuzel, Guillaume Fogliani, Bruno Wulff, Adrien S. L’Huillier, Laurent Kurata, Seikan Ueno, Saneyoshi Isagi, Yuji Tsumura, Yoshihiko Ito, Motomi Appl Plant Sci Primer Note PREMISE OF THE STUDY: Microsatellite markers were developed for Callitris sulcata (Cupressaceae), an endangered conifer species in New Caledonia. METHODS AND RESULTS: Using sequencing by synthesis (SBS) of an RNA-Seq library, 15 polymorphic nuclear and chloroplast microsatellite markers were developed. When evaluated with 48 individuals, these markers showed genetic variations ranging from two to 15 alleles and expected heterozygosity ranging from 0 to 0.881. CONCLUSIONS: These markers will be useful for examining the genetic diversity and structure of remaining wild populations and improving the genetic status of ex situ populations. Botanical Society of America 2015-08-10 /pmc/articles/PMC4542945/ /pubmed/26312198 http://dx.doi.org/10.3732/apps.1500045 Text en © 2015 Sakaguchi et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Sakaguchi, Shota
Lannuzel, Guillaume
Fogliani, Bruno
Wulff, Adrien S.
L’Huillier, Laurent
Kurata, Seikan
Ueno, Saneyoshi
Isagi, Yuji
Tsumura, Yoshihiko
Ito, Motomi
Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae)(1)
title Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae)(1)
title_full Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae)(1)
title_fullStr Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae)(1)
title_full_unstemmed Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae)(1)
title_short Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae)(1)
title_sort development of nuclear and chloroplast microsatellite markers for the endangered conifer callitris sulcata (cupressaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542945/
https://www.ncbi.nlm.nih.gov/pubmed/26312198
http://dx.doi.org/10.3732/apps.1500045
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