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Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick(GFP )Neural Tube Grafting and Carbocyanine Dye DiI Injection
All developing organs need to be connected to both the nervous system (for sensory and motor control) as well as the vascular system (for gas exchange, fluid and nutrient supply). Consequently both the nervous and vascular systems develop alongside each other and share striking similarities in their...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542995/ https://www.ncbi.nlm.nih.gov/pubmed/26065540 http://dx.doi.org/10.3791/52514 |
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author | Delalande, Jean-Marie Thapar, Nikhil Burns, Alan J. |
author_facet | Delalande, Jean-Marie Thapar, Nikhil Burns, Alan J. |
author_sort | Delalande, Jean-Marie |
collection | PubMed |
description | All developing organs need to be connected to both the nervous system (for sensory and motor control) as well as the vascular system (for gas exchange, fluid and nutrient supply). Consequently both the nervous and vascular systems develop alongside each other and share striking similarities in their branching architecture. Here we report embryonic manipulations that allow us to study the simultaneous development of neural crest-derived nervous tissue (in this case the enteric nervous system), and the vascular system. This is achieved by generating chicken chimeras via transplantation of discrete segments of the neural tube, and associated neural crest, combined with vascular DiI injection in the same embryo. Our method uses transgenic chick(GFP) embryos for intraspecies grafting, making the transplant technique more powerful than the classical quail-chick interspecies grafting protocol used with great effect since the 1970s. Chick(GFP)-chick intraspecies grafting facilitates imaging of transplanted cells and their projections in intact tissues, and eliminates any potential bias in cell development linked to species differences. This method takes full advantage of the ease of access of the avian embryo (compared with other vertebrate embryos) to study the co-development of the enteric nervous system and the vascular system. |
format | Online Article Text |
id | pubmed-4542995 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MyJove Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-45429952015-09-01 Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick(GFP )Neural Tube Grafting and Carbocyanine Dye DiI Injection Delalande, Jean-Marie Thapar, Nikhil Burns, Alan J. J Vis Exp Developmental Biology All developing organs need to be connected to both the nervous system (for sensory and motor control) as well as the vascular system (for gas exchange, fluid and nutrient supply). Consequently both the nervous and vascular systems develop alongside each other and share striking similarities in their branching architecture. Here we report embryonic manipulations that allow us to study the simultaneous development of neural crest-derived nervous tissue (in this case the enteric nervous system), and the vascular system. This is achieved by generating chicken chimeras via transplantation of discrete segments of the neural tube, and associated neural crest, combined with vascular DiI injection in the same embryo. Our method uses transgenic chick(GFP) embryos for intraspecies grafting, making the transplant technique more powerful than the classical quail-chick interspecies grafting protocol used with great effect since the 1970s. Chick(GFP)-chick intraspecies grafting facilitates imaging of transplanted cells and their projections in intact tissues, and eliminates any potential bias in cell development linked to species differences. This method takes full advantage of the ease of access of the avian embryo (compared with other vertebrate embryos) to study the co-development of the enteric nervous system and the vascular system. MyJove Corporation 2015-05-28 /pmc/articles/PMC4542995/ /pubmed/26065540 http://dx.doi.org/10.3791/52514 Text en Copyright © 2015, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial License, which permits non-commercial use, distribution, and reproduction, provided the original work is properly cited. |
spellingShingle | Developmental Biology Delalande, Jean-Marie Thapar, Nikhil Burns, Alan J. Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick(GFP )Neural Tube Grafting and Carbocyanine Dye DiI Injection |
title | Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick(GFP )Neural Tube Grafting and Carbocyanine Dye DiI Injection |
title_full | Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick(GFP )Neural Tube Grafting and Carbocyanine Dye DiI Injection |
title_fullStr | Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick(GFP )Neural Tube Grafting and Carbocyanine Dye DiI Injection |
title_full_unstemmed | Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick(GFP )Neural Tube Grafting and Carbocyanine Dye DiI Injection |
title_short | Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick(GFP )Neural Tube Grafting and Carbocyanine Dye DiI Injection |
title_sort | dual labeling of neural crest cells and blood vessels within chicken embryos using chick(gfp )neural tube grafting and carbocyanine dye dii injection |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542995/ https://www.ncbi.nlm.nih.gov/pubmed/26065540 http://dx.doi.org/10.3791/52514 |
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