Cargando…
Mitochondria-targeted antioxidant SkQ1 improves impaired dermal wound healing in old mice
The process of skin wound healing is delayed or impaired in aging animals. To investigate the possible role of mitochondrial reactive oxygen species (mtROS) in cutaneous wound healing of aged mice, we have applied the mitochondria-targeted antioxidant SkQ1. The SkQ1 treatment resulted in accelerated...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4543037/ https://www.ncbi.nlm.nih.gov/pubmed/26187706 |
_version_ | 1782386573961068544 |
---|---|
author | Demyanenko, Ilya A. Popova, Ekaterina N. Zakharova, Vlada V. Ilyinskaya, Olga P. Vasilieva, Tamara V. Romashchenko, Valeria P. Fedorov, Artem V. Manskikh, Vasily N. Skulachev, Maxim V. Zinovkin, Roman A. Pletjushkina, Olga Yu. Skulachev, Vladimir P. Chernyak, Boris V. |
author_facet | Demyanenko, Ilya A. Popova, Ekaterina N. Zakharova, Vlada V. Ilyinskaya, Olga P. Vasilieva, Tamara V. Romashchenko, Valeria P. Fedorov, Artem V. Manskikh, Vasily N. Skulachev, Maxim V. Zinovkin, Roman A. Pletjushkina, Olga Yu. Skulachev, Vladimir P. Chernyak, Boris V. |
author_sort | Demyanenko, Ilya A. |
collection | PubMed |
description | The process of skin wound healing is delayed or impaired in aging animals. To investigate the possible role of mitochondrial reactive oxygen species (mtROS) in cutaneous wound healing of aged mice, we have applied the mitochondria-targeted antioxidant SkQ1. The SkQ1 treatment resulted in accelerated resolution of the inflammatory phase, formation of granulation tissue, vascularization and epithelization of the wounds. The wounds of SkQ1-treated mice contained increased amount of myofibroblasts which produce extracellular matrix proteins and growth factors mediating granulation tissue formation. This effect resembled SkQ1-induced differentiation of fibroblasts to myofibroblast, observed earlier in vitro. The Transforming Growth Factor beta (TGFβ)produced by SkQ1-treated fibroblasts was found to stimulated motility of endothelial cells in vitro, an effect which may underlie pro-angiogenic action of SkQ1 in the wounds. In vitro experiments showed that SkQ1 prevented decomposition of VE-cadherin containing contacts and following increase in permeability of endothelial cells monolayer, induced by pro-inflammatory cytokine TNF. Prevention of excessive reaction of endothelium to the pro-inflammatory cytokine(s) might account for anti-inflammatory effect of SkQ1. Our findings point to an important role of mtROS in pathogenesis of age-related chronic wounds. |
format | Online Article Text |
id | pubmed-4543037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-45430372015-08-26 Mitochondria-targeted antioxidant SkQ1 improves impaired dermal wound healing in old mice Demyanenko, Ilya A. Popova, Ekaterina N. Zakharova, Vlada V. Ilyinskaya, Olga P. Vasilieva, Tamara V. Romashchenko, Valeria P. Fedorov, Artem V. Manskikh, Vasily N. Skulachev, Maxim V. Zinovkin, Roman A. Pletjushkina, Olga Yu. Skulachev, Vladimir P. Chernyak, Boris V. Aging (Albany NY) Research Paper The process of skin wound healing is delayed or impaired in aging animals. To investigate the possible role of mitochondrial reactive oxygen species (mtROS) in cutaneous wound healing of aged mice, we have applied the mitochondria-targeted antioxidant SkQ1. The SkQ1 treatment resulted in accelerated resolution of the inflammatory phase, formation of granulation tissue, vascularization and epithelization of the wounds. The wounds of SkQ1-treated mice contained increased amount of myofibroblasts which produce extracellular matrix proteins and growth factors mediating granulation tissue formation. This effect resembled SkQ1-induced differentiation of fibroblasts to myofibroblast, observed earlier in vitro. The Transforming Growth Factor beta (TGFβ)produced by SkQ1-treated fibroblasts was found to stimulated motility of endothelial cells in vitro, an effect which may underlie pro-angiogenic action of SkQ1 in the wounds. In vitro experiments showed that SkQ1 prevented decomposition of VE-cadherin containing contacts and following increase in permeability of endothelial cells monolayer, induced by pro-inflammatory cytokine TNF. Prevention of excessive reaction of endothelium to the pro-inflammatory cytokine(s) might account for anti-inflammatory effect of SkQ1. Our findings point to an important role of mtROS in pathogenesis of age-related chronic wounds. Impact Journals LLC 2015-06-30 /pmc/articles/PMC4543037/ /pubmed/26187706 Text en Copyright: © 2015 Demyanenko et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Demyanenko, Ilya A. Popova, Ekaterina N. Zakharova, Vlada V. Ilyinskaya, Olga P. Vasilieva, Tamara V. Romashchenko, Valeria P. Fedorov, Artem V. Manskikh, Vasily N. Skulachev, Maxim V. Zinovkin, Roman A. Pletjushkina, Olga Yu. Skulachev, Vladimir P. Chernyak, Boris V. Mitochondria-targeted antioxidant SkQ1 improves impaired dermal wound healing in old mice |
title | Mitochondria-targeted antioxidant SkQ1 improves impaired dermal wound healing in old mice |
title_full | Mitochondria-targeted antioxidant SkQ1 improves impaired dermal wound healing in old mice |
title_fullStr | Mitochondria-targeted antioxidant SkQ1 improves impaired dermal wound healing in old mice |
title_full_unstemmed | Mitochondria-targeted antioxidant SkQ1 improves impaired dermal wound healing in old mice |
title_short | Mitochondria-targeted antioxidant SkQ1 improves impaired dermal wound healing in old mice |
title_sort | mitochondria-targeted antioxidant skq1 improves impaired dermal wound healing in old mice |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4543037/ https://www.ncbi.nlm.nih.gov/pubmed/26187706 |
work_keys_str_mv | AT demyanenkoilyaa mitochondriatargetedantioxidantskq1improvesimpaireddermalwoundhealinginoldmice AT popovaekaterinan mitochondriatargetedantioxidantskq1improvesimpaireddermalwoundhealinginoldmice AT zakharovavladav mitochondriatargetedantioxidantskq1improvesimpaireddermalwoundhealinginoldmice AT ilyinskayaolgap mitochondriatargetedantioxidantskq1improvesimpaireddermalwoundhealinginoldmice AT vasilievatamarav mitochondriatargetedantioxidantskq1improvesimpaireddermalwoundhealinginoldmice AT romashchenkovaleriap mitochondriatargetedantioxidantskq1improvesimpaireddermalwoundhealinginoldmice AT fedorovartemv mitochondriatargetedantioxidantskq1improvesimpaireddermalwoundhealinginoldmice AT manskikhvasilyn mitochondriatargetedantioxidantskq1improvesimpaireddermalwoundhealinginoldmice AT skulachevmaximv mitochondriatargetedantioxidantskq1improvesimpaireddermalwoundhealinginoldmice AT zinovkinromana mitochondriatargetedantioxidantskq1improvesimpaireddermalwoundhealinginoldmice AT pletjushkinaolgayu mitochondriatargetedantioxidantskq1improvesimpaireddermalwoundhealinginoldmice AT skulachevvladimirp mitochondriatargetedantioxidantskq1improvesimpaireddermalwoundhealinginoldmice AT chernyakborisv mitochondriatargetedantioxidantskq1improvesimpaireddermalwoundhealinginoldmice |