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Electroporation Knows No Boundaries: The Use of Electrostimulation for siRNA Delivery in Cells and Tissues

The discovery of RNA interference (RNAi) has enabled several breakthrough discoveries in the area of functional genomics. The RNAi technology has emerged as one of the major tools for drug target identification and has been steadily improved to allow gene manipulation in cell lines, tissues, and who...

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Detalles Bibliográficos
Autores principales: Luft, Christin, Ketteler, Robin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4543902/
https://www.ncbi.nlm.nih.gov/pubmed/25851034
http://dx.doi.org/10.1177/1087057115579638
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author Luft, Christin
Ketteler, Robin
author_facet Luft, Christin
Ketteler, Robin
author_sort Luft, Christin
collection PubMed
description The discovery of RNA interference (RNAi) has enabled several breakthrough discoveries in the area of functional genomics. The RNAi technology has emerged as one of the major tools for drug target identification and has been steadily improved to allow gene manipulation in cell lines, tissues, and whole organisms. One of the major hurdles for the use of RNAi in high-throughput screening has been delivery to cells and tissues. Some cell types are refractory to high-efficiency transfection with standard methods such as lipofection or calcium phosphate precipitation and require different means. Electroporation is a powerful and versatile method for delivery of RNA, DNA, peptides, and small molecules into cell lines and primary cells, as well as whole tissues and organisms. Of particular interest is the use of electroporation for delivery of small interfering RNA oligonucleotides and clustered regularly interspaced short palindromic repeats/Cas9 plasmid vectors in high-throughput screening and for therapeutic applications. Here, we will review the use of electroporation in high-throughput screening in cell lines and tissues.
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spelling pubmed-45439022015-08-31 Electroporation Knows No Boundaries: The Use of Electrostimulation for siRNA Delivery in Cells and Tissues Luft, Christin Ketteler, Robin J Biomol Screen Article The discovery of RNA interference (RNAi) has enabled several breakthrough discoveries in the area of functional genomics. The RNAi technology has emerged as one of the major tools for drug target identification and has been steadily improved to allow gene manipulation in cell lines, tissues, and whole organisms. One of the major hurdles for the use of RNAi in high-throughput screening has been delivery to cells and tissues. Some cell types are refractory to high-efficiency transfection with standard methods such as lipofection or calcium phosphate precipitation and require different means. Electroporation is a powerful and versatile method for delivery of RNA, DNA, peptides, and small molecules into cell lines and primary cells, as well as whole tissues and organisms. Of particular interest is the use of electroporation for delivery of small interfering RNA oligonucleotides and clustered regularly interspaced short palindromic repeats/Cas9 plasmid vectors in high-throughput screening and for therapeutic applications. Here, we will review the use of electroporation in high-throughput screening in cell lines and tissues. SAGE Publications 2015-09 /pmc/articles/PMC4543902/ /pubmed/25851034 http://dx.doi.org/10.1177/1087057115579638 Text en © 2015 Society for Laboratory Automation and Screening http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution 3.0 License (http://www.creativecommons.org/licenses/by/3.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (http://www.uk.sagepub.com/aboutus/openaccess.htm).
spellingShingle Article
Luft, Christin
Ketteler, Robin
Electroporation Knows No Boundaries: The Use of Electrostimulation for siRNA Delivery in Cells and Tissues
title Electroporation Knows No Boundaries: The Use of Electrostimulation for siRNA Delivery in Cells and Tissues
title_full Electroporation Knows No Boundaries: The Use of Electrostimulation for siRNA Delivery in Cells and Tissues
title_fullStr Electroporation Knows No Boundaries: The Use of Electrostimulation for siRNA Delivery in Cells and Tissues
title_full_unstemmed Electroporation Knows No Boundaries: The Use of Electrostimulation for siRNA Delivery in Cells and Tissues
title_short Electroporation Knows No Boundaries: The Use of Electrostimulation for siRNA Delivery in Cells and Tissues
title_sort electroporation knows no boundaries: the use of electrostimulation for sirna delivery in cells and tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4543902/
https://www.ncbi.nlm.nih.gov/pubmed/25851034
http://dx.doi.org/10.1177/1087057115579638
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