Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping

We introduce a non-invasive approach for optogenetic regulation in biological cells through highly scattering skull tissue using wavefront shaping. The wavefront of the incident light was systematically controlled using a spatial light modulator in order to overcome multiple light-scattering in a mo...

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Detalles Bibliográficos
Autores principales: Yoon, Jonghee, Lee, Minji, Lee, KyeoReh, Kim, Nury, Kim, Jin Man, Park, Jongchan, Yu, Hyeonseung, Choi, Chulhee, Heo, Won Do, Park, YongKeun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4543936/
https://www.ncbi.nlm.nih.gov/pubmed/26293590
http://dx.doi.org/10.1038/srep13289
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author Yoon, Jonghee
Lee, Minji
Lee, KyeoReh
Kim, Nury
Kim, Jin Man
Park, Jongchan
Yu, Hyeonseung
Choi, Chulhee
Heo, Won Do
Park, YongKeun
author_facet Yoon, Jonghee
Lee, Minji
Lee, KyeoReh
Kim, Nury
Kim, Jin Man
Park, Jongchan
Yu, Hyeonseung
Choi, Chulhee
Heo, Won Do
Park, YongKeun
author_sort Yoon, Jonghee
collection PubMed
description We introduce a non-invasive approach for optogenetic regulation in biological cells through highly scattering skull tissue using wavefront shaping. The wavefront of the incident light was systematically controlled using a spatial light modulator in order to overcome multiple light-scattering in a mouse skull layer and to focus light on the target cells. We demonstrate that illumination with shaped waves enables spatiotemporal regulation of intracellular Ca(2+) level at the individual-cell level.
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spelling pubmed-45439362015-09-01 Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping Yoon, Jonghee Lee, Minji Lee, KyeoReh Kim, Nury Kim, Jin Man Park, Jongchan Yu, Hyeonseung Choi, Chulhee Heo, Won Do Park, YongKeun Sci Rep Article We introduce a non-invasive approach for optogenetic regulation in biological cells through highly scattering skull tissue using wavefront shaping. The wavefront of the incident light was systematically controlled using a spatial light modulator in order to overcome multiple light-scattering in a mouse skull layer and to focus light on the target cells. We demonstrate that illumination with shaped waves enables spatiotemporal regulation of intracellular Ca(2+) level at the individual-cell level. Nature Publishing Group 2015-08-21 /pmc/articles/PMC4543936/ /pubmed/26293590 http://dx.doi.org/10.1038/srep13289 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Yoon, Jonghee
Lee, Minji
Lee, KyeoReh
Kim, Nury
Kim, Jin Man
Park, Jongchan
Yu, Hyeonseung
Choi, Chulhee
Heo, Won Do
Park, YongKeun
Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
title Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
title_full Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
title_fullStr Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
title_full_unstemmed Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
title_short Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
title_sort optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4543936/
https://www.ncbi.nlm.nih.gov/pubmed/26293590
http://dx.doi.org/10.1038/srep13289
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