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The Amborella vacuolar processing enzyme family

Most vacuolar proteins are synthesized on rough endoplasmic reticulum as proprotein precursors and then transported to the vacuoles, where they are converted into their respective mature forms by vacuolar processing enzymes (VPEs). In the case of the seed storage proteins, this process is of major i...

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Autores principales: Poncet, Valérie, Scutt, Charlie, Tournebize, Rémi, Villegente, Matthieu, Cueff, Gwendal, Rajjou, Loïc, Balliau, Thierry, Zivy, Michel, Fogliani, Bruno, Job, Claudette, de Kochko, Alexandre, Sarramegna-Burtet, Valérie, Job, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4544213/
https://www.ncbi.nlm.nih.gov/pubmed/26347753
http://dx.doi.org/10.3389/fpls.2015.00618
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author Poncet, Valérie
Scutt, Charlie
Tournebize, Rémi
Villegente, Matthieu
Cueff, Gwendal
Rajjou, Loïc
Balliau, Thierry
Zivy, Michel
Fogliani, Bruno
Job, Claudette
de Kochko, Alexandre
Sarramegna-Burtet, Valérie
Job, Dominique
author_facet Poncet, Valérie
Scutt, Charlie
Tournebize, Rémi
Villegente, Matthieu
Cueff, Gwendal
Rajjou, Loïc
Balliau, Thierry
Zivy, Michel
Fogliani, Bruno
Job, Claudette
de Kochko, Alexandre
Sarramegna-Burtet, Valérie
Job, Dominique
author_sort Poncet, Valérie
collection PubMed
description Most vacuolar proteins are synthesized on rough endoplasmic reticulum as proprotein precursors and then transported to the vacuoles, where they are converted into their respective mature forms by vacuolar processing enzymes (VPEs). In the case of the seed storage proteins, this process is of major importance, as it conditions the establishment of vigorous seedlings. Toward the goal of identifying proteome signatures that could be associated with the origin and early diversification of angiosperms, we previously characterized the 11S-legumin-type seed storage proteins from Amborella trichopoda, a rainforest shrub endemic to New Caledonia that is also the probable sister to all other angiosperms (Amborella Genome Project, 2013). In the present study, proteomic and genomic approaches were used to characterize the VPE family in this species. Three genes were found to encode VPEs in the Amborella's genome. Phylogenetic analyses showed that the Amborella sequences grouped within two major clades of angiosperm VPEs, indicating that the duplication that generated the ancestors of these clades occurred before the most recent common ancestor of living angiosperms. A further important duplication within the VPE family appears to have occurred in common ancestor of the core eudicots, while many more recent duplications have also occurred in specific taxa, including both Arabidopsis thaliana and Amborella. An analysis of natural genetic variation for each of the three Amborella VPE genes revealed the absence of selective forces acting on intronic and exonic single-nucleotide polymorphisms among several natural Amborella populations in New Caledonia.
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spelling pubmed-45442132015-09-07 The Amborella vacuolar processing enzyme family Poncet, Valérie Scutt, Charlie Tournebize, Rémi Villegente, Matthieu Cueff, Gwendal Rajjou, Loïc Balliau, Thierry Zivy, Michel Fogliani, Bruno Job, Claudette de Kochko, Alexandre Sarramegna-Burtet, Valérie Job, Dominique Front Plant Sci Plant Science Most vacuolar proteins are synthesized on rough endoplasmic reticulum as proprotein precursors and then transported to the vacuoles, where they are converted into their respective mature forms by vacuolar processing enzymes (VPEs). In the case of the seed storage proteins, this process is of major importance, as it conditions the establishment of vigorous seedlings. Toward the goal of identifying proteome signatures that could be associated with the origin and early diversification of angiosperms, we previously characterized the 11S-legumin-type seed storage proteins from Amborella trichopoda, a rainforest shrub endemic to New Caledonia that is also the probable sister to all other angiosperms (Amborella Genome Project, 2013). In the present study, proteomic and genomic approaches were used to characterize the VPE family in this species. Three genes were found to encode VPEs in the Amborella's genome. Phylogenetic analyses showed that the Amborella sequences grouped within two major clades of angiosperm VPEs, indicating that the duplication that generated the ancestors of these clades occurred before the most recent common ancestor of living angiosperms. A further important duplication within the VPE family appears to have occurred in common ancestor of the core eudicots, while many more recent duplications have also occurred in specific taxa, including both Arabidopsis thaliana and Amborella. An analysis of natural genetic variation for each of the three Amborella VPE genes revealed the absence of selective forces acting on intronic and exonic single-nucleotide polymorphisms among several natural Amborella populations in New Caledonia. Frontiers Media S.A. 2015-08-21 /pmc/articles/PMC4544213/ /pubmed/26347753 http://dx.doi.org/10.3389/fpls.2015.00618 Text en Copyright © 2015 Poncet, Scutt, Tournebize, Villegente, Cueff, Rajjou, Balliau, Zivy, Fogliani, Job, de Kochko, Sarramegna-Burtet and Job. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Poncet, Valérie
Scutt, Charlie
Tournebize, Rémi
Villegente, Matthieu
Cueff, Gwendal
Rajjou, Loïc
Balliau, Thierry
Zivy, Michel
Fogliani, Bruno
Job, Claudette
de Kochko, Alexandre
Sarramegna-Burtet, Valérie
Job, Dominique
The Amborella vacuolar processing enzyme family
title The Amborella vacuolar processing enzyme family
title_full The Amborella vacuolar processing enzyme family
title_fullStr The Amborella vacuolar processing enzyme family
title_full_unstemmed The Amborella vacuolar processing enzyme family
title_short The Amborella vacuolar processing enzyme family
title_sort amborella vacuolar processing enzyme family
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4544213/
https://www.ncbi.nlm.nih.gov/pubmed/26347753
http://dx.doi.org/10.3389/fpls.2015.00618
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