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MOLECULAR IDENTIFICATION AND ANTIMICROBIAL RESISTANCE PATTERN OF SEVEN CLINICAL ISOLATES OF Nocardia spp. IN BRAZIL

Nocardia is a ubiquitous microorganism related to pyogranulomatous infection, which is difficult to treat in humans and animals. The occurrence of the disease is on the rise in many countries due to an increase in immunosuppressive diseases and treatments. This report of cases from Brazil presents t...

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Autores principales: CONDAS, Larissa Anuska Zeni, RIBEIRO, Márcio Garcia, MURO, Marisol Domingues, de VARGAS, Agueda Palmira Castagna, MATSUZAWA, Tetsuhiro, YAZAWA, Katsukiyo, SIQUEIRA, Amanda Keller, SALERNO, Tatiana, LARA, Gustavo Henrique Batista, RISSETI, Rafaela Mastrangelo, FERREIRA, Karen Spadari, GONOI, Tohru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Revista do Instituto de Medicina Tropical de São Paulo 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4544251/
https://www.ncbi.nlm.nih.gov/pubmed/26200967
http://dx.doi.org/10.1590/S0036-46652015000300012
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author CONDAS, Larissa Anuska Zeni
RIBEIRO, Márcio Garcia
MURO, Marisol Domingues
de VARGAS, Agueda Palmira Castagna
MATSUZAWA, Tetsuhiro
YAZAWA, Katsukiyo
SIQUEIRA, Amanda Keller
SALERNO, Tatiana
LARA, Gustavo Henrique Batista
RISSETI, Rafaela Mastrangelo
FERREIRA, Karen Spadari
GONOI, Tohru
author_facet CONDAS, Larissa Anuska Zeni
RIBEIRO, Márcio Garcia
MURO, Marisol Domingues
de VARGAS, Agueda Palmira Castagna
MATSUZAWA, Tetsuhiro
YAZAWA, Katsukiyo
SIQUEIRA, Amanda Keller
SALERNO, Tatiana
LARA, Gustavo Henrique Batista
RISSETI, Rafaela Mastrangelo
FERREIRA, Karen Spadari
GONOI, Tohru
author_sort CONDAS, Larissa Anuska Zeni
collection PubMed
description Nocardia is a ubiquitous microorganism related to pyogranulomatous infection, which is difficult to treat in humans and animals. The occurrence of the disease is on the rise in many countries due to an increase in immunosuppressive diseases and treatments. This report of cases from Brazil presents the genotypic characterization and the antimicrobial susceptibility pattern using the disk-diffusion method and inhibitory minimal concentration with E-test® strips. In summary, this report focuses on infections in young adult men, of which three cases were cutaneous, two pulmonary, one neurological and one systemic. The pulmonary, neurological and systemic cases were attributed to immunosuppressive diseases or treatments. Sequencing analysis of the 16S rRNA segments (1491 bp) identified four isolates of Nocardia farcinica, two isolates of Nocardia nova and one isolate of Nocardia asiatica. N. farcinica was involved in two cutaneous, one systemic and other pulmonary cases; N. nova was involved in one neurological and one pulmonary case; and Nocardia asiatica in one cutaneous case. The disk-diffusion antimicrobial susceptibility test showed that the most effective antimicrobials were amikacin (100%), amoxicillin/clavulanate (100%), cephalexin (100%) and ceftiofur (100%), while isolates had presented most resistance to gentamicin (43%), sulfamethoxazole/trimethoprim (43%) and ampicillin (29%). However, on the inhibitory minimal concentration test (MIC test), only one of the four isolates of Nocardia farcinica was resistant to sulfamethoxazole/trimethoprim.
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spelling pubmed-45442512015-08-25 MOLECULAR IDENTIFICATION AND ANTIMICROBIAL RESISTANCE PATTERN OF SEVEN CLINICAL ISOLATES OF Nocardia spp. IN BRAZIL CONDAS, Larissa Anuska Zeni RIBEIRO, Márcio Garcia MURO, Marisol Domingues de VARGAS, Agueda Palmira Castagna MATSUZAWA, Tetsuhiro YAZAWA, Katsukiyo SIQUEIRA, Amanda Keller SALERNO, Tatiana LARA, Gustavo Henrique Batista RISSETI, Rafaela Mastrangelo FERREIRA, Karen Spadari GONOI, Tohru Rev Inst Med Trop Sao Paulo Nocardiosis Nocardia is a ubiquitous microorganism related to pyogranulomatous infection, which is difficult to treat in humans and animals. The occurrence of the disease is on the rise in many countries due to an increase in immunosuppressive diseases and treatments. This report of cases from Brazil presents the genotypic characterization and the antimicrobial susceptibility pattern using the disk-diffusion method and inhibitory minimal concentration with E-test® strips. In summary, this report focuses on infections in young adult men, of which three cases were cutaneous, two pulmonary, one neurological and one systemic. The pulmonary, neurological and systemic cases were attributed to immunosuppressive diseases or treatments. Sequencing analysis of the 16S rRNA segments (1491 bp) identified four isolates of Nocardia farcinica, two isolates of Nocardia nova and one isolate of Nocardia asiatica. N. farcinica was involved in two cutaneous, one systemic and other pulmonary cases; N. nova was involved in one neurological and one pulmonary case; and Nocardia asiatica in one cutaneous case. The disk-diffusion antimicrobial susceptibility test showed that the most effective antimicrobials were amikacin (100%), amoxicillin/clavulanate (100%), cephalexin (100%) and ceftiofur (100%), while isolates had presented most resistance to gentamicin (43%), sulfamethoxazole/trimethoprim (43%) and ampicillin (29%). However, on the inhibitory minimal concentration test (MIC test), only one of the four isolates of Nocardia farcinica was resistant to sulfamethoxazole/trimethoprim. Revista do Instituto de Medicina Tropical de São Paulo 2015 /pmc/articles/PMC4544251/ /pubmed/26200967 http://dx.doi.org/10.1590/S0036-46652015000300012 Text en http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Nocardiosis
CONDAS, Larissa Anuska Zeni
RIBEIRO, Márcio Garcia
MURO, Marisol Domingues
de VARGAS, Agueda Palmira Castagna
MATSUZAWA, Tetsuhiro
YAZAWA, Katsukiyo
SIQUEIRA, Amanda Keller
SALERNO, Tatiana
LARA, Gustavo Henrique Batista
RISSETI, Rafaela Mastrangelo
FERREIRA, Karen Spadari
GONOI, Tohru
MOLECULAR IDENTIFICATION AND ANTIMICROBIAL RESISTANCE PATTERN OF SEVEN CLINICAL ISOLATES OF Nocardia spp. IN BRAZIL
title MOLECULAR IDENTIFICATION AND ANTIMICROBIAL RESISTANCE PATTERN OF SEVEN CLINICAL ISOLATES OF Nocardia spp. IN BRAZIL
title_full MOLECULAR IDENTIFICATION AND ANTIMICROBIAL RESISTANCE PATTERN OF SEVEN CLINICAL ISOLATES OF Nocardia spp. IN BRAZIL
title_fullStr MOLECULAR IDENTIFICATION AND ANTIMICROBIAL RESISTANCE PATTERN OF SEVEN CLINICAL ISOLATES OF Nocardia spp. IN BRAZIL
title_full_unstemmed MOLECULAR IDENTIFICATION AND ANTIMICROBIAL RESISTANCE PATTERN OF SEVEN CLINICAL ISOLATES OF Nocardia spp. IN BRAZIL
title_short MOLECULAR IDENTIFICATION AND ANTIMICROBIAL RESISTANCE PATTERN OF SEVEN CLINICAL ISOLATES OF Nocardia spp. IN BRAZIL
title_sort molecular identification and antimicrobial resistance pattern of seven clinical isolates of nocardia spp. in brazil
topic Nocardiosis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4544251/
https://www.ncbi.nlm.nih.gov/pubmed/26200967
http://dx.doi.org/10.1590/S0036-46652015000300012
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