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Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
Synthetic materials are known to initiate clinical complications such as inflammation, stenosis, and infections when implanted as vascular substitutes. Collagen has been extensively used for a wide range of biomedical applications and is considered a valid alternative to synthetic materials due to i...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545069/ https://www.ncbi.nlm.nih.gov/pubmed/26132527 http://dx.doi.org/10.3791/52812 |
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author | Meghezi, Sébastien Seifu, Dawit G. Bono, Nina Unsworth, Larry Mequanint, Kibret Mantovani, Diego |
author_facet | Meghezi, Sébastien Seifu, Dawit G. Bono, Nina Unsworth, Larry Mequanint, Kibret Mantovani, Diego |
author_sort | Meghezi, Sébastien |
collection | PubMed |
description | Synthetic materials are known to initiate clinical complications such as inflammation, stenosis, and infections when implanted as vascular substitutes. Collagen has been extensively used for a wide range of biomedical applications and is considered a valid alternative to synthetic materials due to its inherent biocompatibility (i.e., low antigenicity, inflammation, and cytotoxic responses). However, the limited mechanical properties and the related low hand-ability of collagen gels have hampered their use as scaffold materials for vascular tissue engineering. Therefore, the rationale behind this work was first to engineer cellularized collagen gels into a tubular-shaped geometry and second to enhance smooth muscle cells driven reorganization of collagen matrix to obtain tissues stiff enough to be handled. The strategy described here is based on the direct assembling of collagen and smooth muscle cells (construct) in a 3D cylindrical geometry with the use of a molding technique. This process requires a maturation period, during which the constructs are cultured in a bioreactor under static conditions (without applied external dynamic mechanical constraints) for 1 or 2 weeks. The “static bioreactor” provides a monitored and controlled sterile environment (pH, temperature, gas exchange, nutrient supply and waste removal) to the constructs. During culture period, thickness measurements were performed to evaluate the cells-driven remodeling of the collagen matrix, and glucose consumption and lactate production rates were measured to monitor the cells metabolic activity. Finally, mechanical and viscoelastic properties were assessed for the resulting tubular constructs. To this end, specific protocols and a focused know-how (manipulation, gripping, working in hydrated environment, and so on) were developed to characterize the engineered tissues. |
format | Online Article Text |
id | pubmed-4545069 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MyJove Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-45450692015-09-03 Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration Meghezi, Sébastien Seifu, Dawit G. Bono, Nina Unsworth, Larry Mequanint, Kibret Mantovani, Diego J Vis Exp Bioengineering Synthetic materials are known to initiate clinical complications such as inflammation, stenosis, and infections when implanted as vascular substitutes. Collagen has been extensively used for a wide range of biomedical applications and is considered a valid alternative to synthetic materials due to its inherent biocompatibility (i.e., low antigenicity, inflammation, and cytotoxic responses). However, the limited mechanical properties and the related low hand-ability of collagen gels have hampered their use as scaffold materials for vascular tissue engineering. Therefore, the rationale behind this work was first to engineer cellularized collagen gels into a tubular-shaped geometry and second to enhance smooth muscle cells driven reorganization of collagen matrix to obtain tissues stiff enough to be handled. The strategy described here is based on the direct assembling of collagen and smooth muscle cells (construct) in a 3D cylindrical geometry with the use of a molding technique. This process requires a maturation period, during which the constructs are cultured in a bioreactor under static conditions (without applied external dynamic mechanical constraints) for 1 or 2 weeks. The “static bioreactor” provides a monitored and controlled sterile environment (pH, temperature, gas exchange, nutrient supply and waste removal) to the constructs. During culture period, thickness measurements were performed to evaluate the cells-driven remodeling of the collagen matrix, and glucose consumption and lactate production rates were measured to monitor the cells metabolic activity. Finally, mechanical and viscoelastic properties were assessed for the resulting tubular constructs. To this end, specific protocols and a focused know-how (manipulation, gripping, working in hydrated environment, and so on) were developed to characterize the engineered tissues. MyJove Corporation 2015-06-16 /pmc/articles/PMC4545069/ /pubmed/26132527 http://dx.doi.org/10.3791/52812 Text en Copyright © 2015, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Bioengineering Meghezi, Sébastien Seifu, Dawit G. Bono, Nina Unsworth, Larry Mequanint, Kibret Mantovani, Diego Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration |
title | Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration |
title_full | Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration |
title_fullStr | Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration |
title_full_unstemmed | Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration |
title_short | Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration |
title_sort | engineering 3d cellularized collagen gels for vascular tissue regeneration |
topic | Bioengineering |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545069/ https://www.ncbi.nlm.nih.gov/pubmed/26132527 http://dx.doi.org/10.3791/52812 |
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