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Isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect

BACKGROUND: Isolation of methyl gamma linolenate from Spirulina platensis using flash chromatography and its apoptosis inducing effect against human lung carcinoma A- 549 cell lines. METHODS: Gamma linolenic acid is an important omega-6 polyunsaturated fatty acid (PUFA) of medicinal interest was iso...

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Autores principales: S., Jubie, S. P., Dhanabal, M. V. N. L., Chaitanya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545820/
https://www.ncbi.nlm.nih.gov/pubmed/26238515
http://dx.doi.org/10.1186/s12906-015-0771-8
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author S., Jubie
S. P., Dhanabal
M. V. N. L., Chaitanya
author_facet S., Jubie
S. P., Dhanabal
M. V. N. L., Chaitanya
author_sort S., Jubie
collection PubMed
description BACKGROUND: Isolation of methyl gamma linolenate from Spirulina platensis using flash chromatography and its apoptosis inducing effect against human lung carcinoma A- 549 cell lines. METHODS: Gamma linolenic acid is an important omega-6 polyunsaturated fatty acid (PUFA) of medicinal interest was isolated from microalgae Spirulina platensis using flash chromatography system (Isolera system) as its methyl ester. The isolated methyl gamma linolenate was characterized by IR, (1)H NMR, (13)C NMR and mass spectral analysis and the data were consistent with the structure. RESULTS: The percentage yield of isolated methyl gamma linolenate is found to be 71 % w/w, which is a very good yield in comparison to other conventional methods. It was subjected to in-vitro cytotoxic screening on A-549 lung cancer cell lines using SRB assay and result was compared with standard rutin. CONCLUSION: It may be concluded that the Flash chromatography system plays a major role in improving the yield for theisolation of methyl gamma linoleate from Spirulina platensis and the isolated molecule is a potent cytotoxicagent towards human lung carcinoma cell lines, however it may be further taken up for an extensive study.
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spelling pubmed-45458202015-08-23 Isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect S., Jubie S. P., Dhanabal M. V. N. L., Chaitanya BMC Complement Altern Med Research Article BACKGROUND: Isolation of methyl gamma linolenate from Spirulina platensis using flash chromatography and its apoptosis inducing effect against human lung carcinoma A- 549 cell lines. METHODS: Gamma linolenic acid is an important omega-6 polyunsaturated fatty acid (PUFA) of medicinal interest was isolated from microalgae Spirulina platensis using flash chromatography system (Isolera system) as its methyl ester. The isolated methyl gamma linolenate was characterized by IR, (1)H NMR, (13)C NMR and mass spectral analysis and the data were consistent with the structure. RESULTS: The percentage yield of isolated methyl gamma linolenate is found to be 71 % w/w, which is a very good yield in comparison to other conventional methods. It was subjected to in-vitro cytotoxic screening on A-549 lung cancer cell lines using SRB assay and result was compared with standard rutin. CONCLUSION: It may be concluded that the Flash chromatography system plays a major role in improving the yield for theisolation of methyl gamma linoleate from Spirulina platensis and the isolated molecule is a potent cytotoxicagent towards human lung carcinoma cell lines, however it may be further taken up for an extensive study. BioMed Central 2015-08-04 /pmc/articles/PMC4545820/ /pubmed/26238515 http://dx.doi.org/10.1186/s12906-015-0771-8 Text en © S. et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (http://creativecommons.org/licenses/by/4.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
S., Jubie
S. P., Dhanabal
M. V. N. L., Chaitanya
Isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect
title Isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect
title_full Isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect
title_fullStr Isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect
title_full_unstemmed Isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect
title_short Isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect
title_sort isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545820/
https://www.ncbi.nlm.nih.gov/pubmed/26238515
http://dx.doi.org/10.1186/s12906-015-0771-8
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