Cargando…
Protective effect of Emblica-officinalis in arsenic induced biochemical alteration and inflammation in mice
Exposure to arsenic in individuals has been found to be associated with immune related problems. In earlier studies, we have demonstrated that amla protects against arsenic induced oxidative stress and apoptosis in thymus and spleen of mice. In continuation to that the present study has therefore be...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545902/ https://www.ncbi.nlm.nih.gov/pubmed/26312203 http://dx.doi.org/10.1186/s40064-015-1227-9 |
_version_ | 1782386809918980096 |
---|---|
author | Singh, Manish K. Yadav, Suraj Singh Yadav, Rajesh Singh Chauhan, Abhishek Katiyar, Devendra Khattri, Sanjay |
author_facet | Singh, Manish K. Yadav, Suraj Singh Yadav, Rajesh Singh Chauhan, Abhishek Katiyar, Devendra Khattri, Sanjay |
author_sort | Singh, Manish K. |
collection | PubMed |
description | Exposure to arsenic in individuals has been found to be associated with immune related problems. In earlier studies, we have demonstrated that amla protects against arsenic induced oxidative stress and apoptosis in thymus and spleen of mice. In continuation to that the present study has therefore been focused to investigate the protective efficacy of amla in arsenic induced inflammation and immunotoxicity in mice. The results showed that arsenic treatment significantly increased serum urea levels (69 %), glucose levels (48 %) and triglyceride levels (66 %) as compared to controls. Mice exposed to arsenic exhibited significant increased in TNF-α (4.3-fold), serum Interleukin-1 beta (threefold), Interleukin-6 (3.8-fold) as compared to controls. Arsenic exposure increased the relative frequency of CD8+ (Tc) cells sub-population (18.9 %) and decreased CD4+ (Th) cells (2.6 %). Arsenic exposure also significantly decreased T (CD3) and B (CD19) cells (21.1 %) as compared to controls. Simultaneously treatment with arsenic and amla significantly inhibited serum urea levels (47 %), glucose levels (50 %) and triglyceride levels (14 %). It also significantly decreased the TNF-α (1.1-fold), levels of IL-1β (1.6-fold), levels of Interleukin-6 (1.3-fold) in serum as compared to those treated with arsenic alone. Simultaneously treatment with arsenic and amla restored the alterations in CD8+ and CD4+ cells and also recovered the damages in B and T sub cells population. Results of the present study clearly indicate that arsenic induced immunotoxicity linked with inflammation has been significantly protected through simultaneous treatment with arsenic and amla that was due to anti-inflammatory and antioxidant activity of amla. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40064-015-1227-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4545902 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-45459022015-08-26 Protective effect of Emblica-officinalis in arsenic induced biochemical alteration and inflammation in mice Singh, Manish K. Yadav, Suraj Singh Yadav, Rajesh Singh Chauhan, Abhishek Katiyar, Devendra Khattri, Sanjay Springerplus Research Exposure to arsenic in individuals has been found to be associated with immune related problems. In earlier studies, we have demonstrated that amla protects against arsenic induced oxidative stress and apoptosis in thymus and spleen of mice. In continuation to that the present study has therefore been focused to investigate the protective efficacy of amla in arsenic induced inflammation and immunotoxicity in mice. The results showed that arsenic treatment significantly increased serum urea levels (69 %), glucose levels (48 %) and triglyceride levels (66 %) as compared to controls. Mice exposed to arsenic exhibited significant increased in TNF-α (4.3-fold), serum Interleukin-1 beta (threefold), Interleukin-6 (3.8-fold) as compared to controls. Arsenic exposure increased the relative frequency of CD8+ (Tc) cells sub-population (18.9 %) and decreased CD4+ (Th) cells (2.6 %). Arsenic exposure also significantly decreased T (CD3) and B (CD19) cells (21.1 %) as compared to controls. Simultaneously treatment with arsenic and amla significantly inhibited serum urea levels (47 %), glucose levels (50 %) and triglyceride levels (14 %). It also significantly decreased the TNF-α (1.1-fold), levels of IL-1β (1.6-fold), levels of Interleukin-6 (1.3-fold) in serum as compared to those treated with arsenic alone. Simultaneously treatment with arsenic and amla restored the alterations in CD8+ and CD4+ cells and also recovered the damages in B and T sub cells population. Results of the present study clearly indicate that arsenic induced immunotoxicity linked with inflammation has been significantly protected through simultaneous treatment with arsenic and amla that was due to anti-inflammatory and antioxidant activity of amla. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40064-015-1227-9) contains supplementary material, which is available to authorized users. Springer International Publishing 2015-08-21 /pmc/articles/PMC4545902/ /pubmed/26312203 http://dx.doi.org/10.1186/s40064-015-1227-9 Text en © Singh et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Singh, Manish K. Yadav, Suraj Singh Yadav, Rajesh Singh Chauhan, Abhishek Katiyar, Devendra Khattri, Sanjay Protective effect of Emblica-officinalis in arsenic induced biochemical alteration and inflammation in mice |
title | Protective effect of Emblica-officinalis in arsenic induced biochemical alteration and inflammation in mice |
title_full | Protective effect of Emblica-officinalis in arsenic induced biochemical alteration and inflammation in mice |
title_fullStr | Protective effect of Emblica-officinalis in arsenic induced biochemical alteration and inflammation in mice |
title_full_unstemmed | Protective effect of Emblica-officinalis in arsenic induced biochemical alteration and inflammation in mice |
title_short | Protective effect of Emblica-officinalis in arsenic induced biochemical alteration and inflammation in mice |
title_sort | protective effect of emblica-officinalis in arsenic induced biochemical alteration and inflammation in mice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545902/ https://www.ncbi.nlm.nih.gov/pubmed/26312203 http://dx.doi.org/10.1186/s40064-015-1227-9 |
work_keys_str_mv | AT singhmanishk protectiveeffectofemblicaofficinalisinarsenicinducedbiochemicalalterationandinflammationinmice AT yadavsurajsingh protectiveeffectofemblicaofficinalisinarsenicinducedbiochemicalalterationandinflammationinmice AT yadavrajeshsingh protectiveeffectofemblicaofficinalisinarsenicinducedbiochemicalalterationandinflammationinmice AT chauhanabhishek protectiveeffectofemblicaofficinalisinarsenicinducedbiochemicalalterationandinflammationinmice AT katiyardevendra protectiveeffectofemblicaofficinalisinarsenicinducedbiochemicalalterationandinflammationinmice AT khattrisanjay protectiveeffectofemblicaofficinalisinarsenicinducedbiochemicalalterationandinflammationinmice |