Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion

Herein, we describe the establishment and characterization of the first mixed-phenotype acute leukemia cell line (JIH-5). The JIH-5 cell line was established from leukemia cells with B lymphoid/myeloid phenotype from a female mixed-phenotype acute leukemia patient. JIH-5 cells exhibit an immunopheno...

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Autores principales: Ping, Nana, Qiu, Huiying, Wang, Qian, Dai, Haiping, Ruan, Changgeng, Ehrentraut, Stefan, Drexler, Hans G., MacLeod, Roderick A. F., Chen, Suning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546145/
https://www.ncbi.nlm.nih.gov/pubmed/26293203
http://dx.doi.org/10.1186/s13045-015-0197-2
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author Ping, Nana
Qiu, Huiying
Wang, Qian
Dai, Haiping
Ruan, Changgeng
Ehrentraut, Stefan
Drexler, Hans G.
MacLeod, Roderick A. F.
Chen, Suning
author_facet Ping, Nana
Qiu, Huiying
Wang, Qian
Dai, Haiping
Ruan, Changgeng
Ehrentraut, Stefan
Drexler, Hans G.
MacLeod, Roderick A. F.
Chen, Suning
author_sort Ping, Nana
collection PubMed
description Herein, we describe the establishment and characterization of the first mixed-phenotype acute leukemia cell line (JIH-5). The JIH-5 cell line was established from leukemia cells with B lymphoid/myeloid phenotype from a female mixed-phenotype acute leukemia patient. JIH-5 cells exhibit an immunophenotype comprised of myeloid and B lymphoid antigens. Whole-exome sequencing revealed somatic mutations in nine genes in JIH-5 cells. Transcriptional sequencing of JIH-5 cells identified EP300-ZNF384 fusion transcript, which is a recurrent alteration in B cell acute lymphoblastic leukemia. Our results suggest that the JIH-5 cell line may serve as a tool for the study of mixed-phenotype acute leukemia or EP300-ZNF384.
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spelling pubmed-45461452015-08-23 Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion Ping, Nana Qiu, Huiying Wang, Qian Dai, Haiping Ruan, Changgeng Ehrentraut, Stefan Drexler, Hans G. MacLeod, Roderick A. F. Chen, Suning J Hematol Oncol Letter to the Editor Herein, we describe the establishment and characterization of the first mixed-phenotype acute leukemia cell line (JIH-5). The JIH-5 cell line was established from leukemia cells with B lymphoid/myeloid phenotype from a female mixed-phenotype acute leukemia patient. JIH-5 cells exhibit an immunophenotype comprised of myeloid and B lymphoid antigens. Whole-exome sequencing revealed somatic mutations in nine genes in JIH-5 cells. Transcriptional sequencing of JIH-5 cells identified EP300-ZNF384 fusion transcript, which is a recurrent alteration in B cell acute lymphoblastic leukemia. Our results suggest that the JIH-5 cell line may serve as a tool for the study of mixed-phenotype acute leukemia or EP300-ZNF384. BioMed Central 2015-08-21 /pmc/articles/PMC4546145/ /pubmed/26293203 http://dx.doi.org/10.1186/s13045-015-0197-2 Text en © Ping et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Letter to the Editor
Ping, Nana
Qiu, Huiying
Wang, Qian
Dai, Haiping
Ruan, Changgeng
Ehrentraut, Stefan
Drexler, Hans G.
MacLeod, Roderick A. F.
Chen, Suning
Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion
title Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion
title_full Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion
title_fullStr Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion
title_full_unstemmed Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion
title_short Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion
title_sort establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with ep300-znf384 fusion
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546145/
https://www.ncbi.nlm.nih.gov/pubmed/26293203
http://dx.doi.org/10.1186/s13045-015-0197-2
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