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Changes in the hemolytic activity of Candida species by common electrolytes

BACKGROUND: Hemolysins are crucial virulence factors which help pathogens to survive and persist in the host. This study investigated whether common electrolytes will affect the hemolysins of Candida species. The hemolysins from 25 Candida isolates were investigated using a plate specially designed...

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Autores principales: Wan, Lei, Luo, Gang, Lu, Haibin, Xuan, Dongying, Cao, Hong, Zhang, Jincai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546287/
https://www.ncbi.nlm.nih.gov/pubmed/26296996
http://dx.doi.org/10.1186/s12866-015-0504-7
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author Wan, Lei
Luo, Gang
Lu, Haibin
Xuan, Dongying
Cao, Hong
Zhang, Jincai
author_facet Wan, Lei
Luo, Gang
Lu, Haibin
Xuan, Dongying
Cao, Hong
Zhang, Jincai
author_sort Wan, Lei
collection PubMed
description BACKGROUND: Hemolysins are crucial virulence factors which help pathogens to survive and persist in the host. This study investigated whether common electrolytes will affect the hemolysins of Candida species. The hemolysins from 25 Candida isolates were investigated using a plate specially designed for Candida species in the presence of three electrolytes, CaCl(2), NaCl and KCl, at different concentrations. The hemolytic activity was determined after 48 h and the hemolytic index was calculated. RESULTS: All three electrolytes caused a decrease in the hemolytic activity. Significant differences existed between varying concentrations of NaCl, while no significant differences existed for the CaCl(2) and KCl groups. Additionally, the peripheral hemolytic index was highly correlated with the hemolytic index (r = 0.656, p < 0.001). CONCLUSIONS: Our findings indicate that electrolytes reduce hemolysis by Candida species and a correlation exists between the peripheral hemolytic index and the translucent hemolytic index.
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spelling pubmed-45462872015-08-23 Changes in the hemolytic activity of Candida species by common electrolytes Wan, Lei Luo, Gang Lu, Haibin Xuan, Dongying Cao, Hong Zhang, Jincai BMC Microbiol Research Article BACKGROUND: Hemolysins are crucial virulence factors which help pathogens to survive and persist in the host. This study investigated whether common electrolytes will affect the hemolysins of Candida species. The hemolysins from 25 Candida isolates were investigated using a plate specially designed for Candida species in the presence of three electrolytes, CaCl(2), NaCl and KCl, at different concentrations. The hemolytic activity was determined after 48 h and the hemolytic index was calculated. RESULTS: All three electrolytes caused a decrease in the hemolytic activity. Significant differences existed between varying concentrations of NaCl, while no significant differences existed for the CaCl(2) and KCl groups. Additionally, the peripheral hemolytic index was highly correlated with the hemolytic index (r = 0.656, p < 0.001). CONCLUSIONS: Our findings indicate that electrolytes reduce hemolysis by Candida species and a correlation exists between the peripheral hemolytic index and the translucent hemolytic index. BioMed Central 2015-08-22 /pmc/articles/PMC4546287/ /pubmed/26296996 http://dx.doi.org/10.1186/s12866-015-0504-7 Text en © Wan et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wan, Lei
Luo, Gang
Lu, Haibin
Xuan, Dongying
Cao, Hong
Zhang, Jincai
Changes in the hemolytic activity of Candida species by common electrolytes
title Changes in the hemolytic activity of Candida species by common electrolytes
title_full Changes in the hemolytic activity of Candida species by common electrolytes
title_fullStr Changes in the hemolytic activity of Candida species by common electrolytes
title_full_unstemmed Changes in the hemolytic activity of Candida species by common electrolytes
title_short Changes in the hemolytic activity of Candida species by common electrolytes
title_sort changes in the hemolytic activity of candida species by common electrolytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546287/
https://www.ncbi.nlm.nih.gov/pubmed/26296996
http://dx.doi.org/10.1186/s12866-015-0504-7
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