Cargando…

Assessment of folate receptor-β expression in human neoplastic tissues

Over-expression of folate receptor alpha on cancer cells has been frequently exploited for delivery of folate-targeted imaging and therapeutic agents to tumors. Because limited information exists on expression of the beta isoform of the folate receptor in human cancers (FR-β), we have evaluated the...

Descripción completa

Detalles Bibliográficos
Autores principales: Shen, Jiayin, Putt, Karson S., Visscher, Daniel W., Murphy, Linda, Cohen, Cynthia, Singhal, Sunil, Sandusky, George, Feng, Yang, Dimitrov, Dimiter S., Low, Philip S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546498/
https://www.ncbi.nlm.nih.gov/pubmed/25909292
_version_ 1782386942808162304
author Shen, Jiayin
Putt, Karson S.
Visscher, Daniel W.
Murphy, Linda
Cohen, Cynthia
Singhal, Sunil
Sandusky, George
Feng, Yang
Dimitrov, Dimiter S.
Low, Philip S.
author_facet Shen, Jiayin
Putt, Karson S.
Visscher, Daniel W.
Murphy, Linda
Cohen, Cynthia
Singhal, Sunil
Sandusky, George
Feng, Yang
Dimitrov, Dimiter S.
Low, Philip S.
author_sort Shen, Jiayin
collection PubMed
description Over-expression of folate receptor alpha on cancer cells has been frequently exploited for delivery of folate-targeted imaging and therapeutic agents to tumors. Because limited information exists on expression of the beta isoform of the folate receptor in human cancers (FR-β), we have evaluated the immunohistochemical staining pattern of FR-β in 992 tumor sections from 20 different human cancer types using a new anti-human FR-β monoclonal antibody. FR-β expression was shown to be more pronounced in cells within the stroma, primarily macrophages and macrophage-like cells than cancer cells in every cancer type studied. Moreover, FR-β expression in both cancer and stromal cells was found to be statistically more prominent in females than males. A significant positive correlation was also observed between FR-β expression on stromal cells and both the stage of the cancer and the presence of lymph node metastases. Based on these data we conclude FR-β may constitute a good target for specific delivery of therapeutic agents to activated macrophages and that accumulation of FR-β positive macrophages in the stroma could serve as a useful indicator of a tumor's metastatic potential.
format Online
Article
Text
id pubmed-4546498
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-45464982015-08-27 Assessment of folate receptor-β expression in human neoplastic tissues Shen, Jiayin Putt, Karson S. Visscher, Daniel W. Murphy, Linda Cohen, Cynthia Singhal, Sunil Sandusky, George Feng, Yang Dimitrov, Dimiter S. Low, Philip S. Oncotarget Research Paper Over-expression of folate receptor alpha on cancer cells has been frequently exploited for delivery of folate-targeted imaging and therapeutic agents to tumors. Because limited information exists on expression of the beta isoform of the folate receptor in human cancers (FR-β), we have evaluated the immunohistochemical staining pattern of FR-β in 992 tumor sections from 20 different human cancer types using a new anti-human FR-β monoclonal antibody. FR-β expression was shown to be more pronounced in cells within the stroma, primarily macrophages and macrophage-like cells than cancer cells in every cancer type studied. Moreover, FR-β expression in both cancer and stromal cells was found to be statistically more prominent in females than males. A significant positive correlation was also observed between FR-β expression on stromal cells and both the stage of the cancer and the presence of lymph node metastases. Based on these data we conclude FR-β may constitute a good target for specific delivery of therapeutic agents to activated macrophages and that accumulation of FR-β positive macrophages in the stroma could serve as a useful indicator of a tumor's metastatic potential. Impact Journals LLC 2015-03-30 /pmc/articles/PMC4546498/ /pubmed/25909292 Text en Copyright: © 2015 Shen et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Shen, Jiayin
Putt, Karson S.
Visscher, Daniel W.
Murphy, Linda
Cohen, Cynthia
Singhal, Sunil
Sandusky, George
Feng, Yang
Dimitrov, Dimiter S.
Low, Philip S.
Assessment of folate receptor-β expression in human neoplastic tissues
title Assessment of folate receptor-β expression in human neoplastic tissues
title_full Assessment of folate receptor-β expression in human neoplastic tissues
title_fullStr Assessment of folate receptor-β expression in human neoplastic tissues
title_full_unstemmed Assessment of folate receptor-β expression in human neoplastic tissues
title_short Assessment of folate receptor-β expression in human neoplastic tissues
title_sort assessment of folate receptor-β expression in human neoplastic tissues
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546498/
https://www.ncbi.nlm.nih.gov/pubmed/25909292
work_keys_str_mv AT shenjiayin assessmentoffolatereceptorbexpressioninhumanneoplastictissues
AT puttkarsons assessmentoffolatereceptorbexpressioninhumanneoplastictissues
AT visscherdanielw assessmentoffolatereceptorbexpressioninhumanneoplastictissues
AT murphylinda assessmentoffolatereceptorbexpressioninhumanneoplastictissues
AT cohencynthia assessmentoffolatereceptorbexpressioninhumanneoplastictissues
AT singhalsunil assessmentoffolatereceptorbexpressioninhumanneoplastictissues
AT sanduskygeorge assessmentoffolatereceptorbexpressioninhumanneoplastictissues
AT fengyang assessmentoffolatereceptorbexpressioninhumanneoplastictissues
AT dimitrovdimiters assessmentoffolatereceptorbexpressioninhumanneoplastictissues
AT lowphilips assessmentoffolatereceptorbexpressioninhumanneoplastictissues