Cargando…
Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry
BACKGROUND: Cytomegalovirus (CMV) is the most common infectious cause of mental disability in newborns in developed countries. There is an urgent need to establish an early detection and high-throughput screening method for CMV infection using portable detection devices. METHODS: An antibody analysi...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546680/ https://www.ncbi.nlm.nih.gov/pubmed/26295458 http://dx.doi.org/10.1371/journal.pone.0136253 |
_version_ | 1782386969499664384 |
---|---|
author | Sun, Hongliu Qi, Cai Niu, Yu Kang, Tengfei Wei, Yongxin Jin, Gang Dong, Xianzhi Wang, Chunhua Zhu, Wei |
author_facet | Sun, Hongliu Qi, Cai Niu, Yu Kang, Tengfei Wei, Yongxin Jin, Gang Dong, Xianzhi Wang, Chunhua Zhu, Wei |
author_sort | Sun, Hongliu |
collection | PubMed |
description | BACKGROUND: Cytomegalovirus (CMV) is the most common infectious cause of mental disability in newborns in developed countries. There is an urgent need to establish an early detection and high-throughput screening method for CMV infection using portable detection devices. METHODS: An antibody analysis method is reported for the detection and identification of CMV antibodies in serum using a biosensor based on high spatial resolution imaging ellipsometry (BIE). CMV antigen (CMV-3A) was immobilized on silicon wafers and used to capture CMV antibodies in serum. An antibody against human immunoglobulin G (anti-IgG) was used to confirm the IgG antibody against CMV captured by the CMV-3A. RESULTS: Our results show that this assay is rapid and specific for the identification of IgG antibody against CMV. Further, patient serum was quantitatively assessed using the standard curve method, and the quantitative results were in agreement with the enzyme-linked immunosorbent assay. The CMV antibody detection sensitivity of BIE reached 0.01 IU/mL. CONCLUSIONS: This novel biosensor may be a valuable diagnostic tool for analysis of IgG antibody against CMV during CMV infection screening. |
format | Online Article Text |
id | pubmed-4546680 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45466802015-09-01 Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry Sun, Hongliu Qi, Cai Niu, Yu Kang, Tengfei Wei, Yongxin Jin, Gang Dong, Xianzhi Wang, Chunhua Zhu, Wei PLoS One Research Article BACKGROUND: Cytomegalovirus (CMV) is the most common infectious cause of mental disability in newborns in developed countries. There is an urgent need to establish an early detection and high-throughput screening method for CMV infection using portable detection devices. METHODS: An antibody analysis method is reported for the detection and identification of CMV antibodies in serum using a biosensor based on high spatial resolution imaging ellipsometry (BIE). CMV antigen (CMV-3A) was immobilized on silicon wafers and used to capture CMV antibodies in serum. An antibody against human immunoglobulin G (anti-IgG) was used to confirm the IgG antibody against CMV captured by the CMV-3A. RESULTS: Our results show that this assay is rapid and specific for the identification of IgG antibody against CMV. Further, patient serum was quantitatively assessed using the standard curve method, and the quantitative results were in agreement with the enzyme-linked immunosorbent assay. The CMV antibody detection sensitivity of BIE reached 0.01 IU/mL. CONCLUSIONS: This novel biosensor may be a valuable diagnostic tool for analysis of IgG antibody against CMV during CMV infection screening. Public Library of Science 2015-08-21 /pmc/articles/PMC4546680/ /pubmed/26295458 http://dx.doi.org/10.1371/journal.pone.0136253 Text en © 2015 Sun et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Sun, Hongliu Qi, Cai Niu, Yu Kang, Tengfei Wei, Yongxin Jin, Gang Dong, Xianzhi Wang, Chunhua Zhu, Wei Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry |
title | Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry |
title_full | Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry |
title_fullStr | Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry |
title_full_unstemmed | Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry |
title_short | Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry |
title_sort | detection of cytomegalovirus antibodies using a biosensor based on imaging ellipsometry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546680/ https://www.ncbi.nlm.nih.gov/pubmed/26295458 http://dx.doi.org/10.1371/journal.pone.0136253 |
work_keys_str_mv | AT sunhongliu detectionofcytomegalovirusantibodiesusingabiosensorbasedonimagingellipsometry AT qicai detectionofcytomegalovirusantibodiesusingabiosensorbasedonimagingellipsometry AT niuyu detectionofcytomegalovirusantibodiesusingabiosensorbasedonimagingellipsometry AT kangtengfei detectionofcytomegalovirusantibodiesusingabiosensorbasedonimagingellipsometry AT weiyongxin detectionofcytomegalovirusantibodiesusingabiosensorbasedonimagingellipsometry AT jingang detectionofcytomegalovirusantibodiesusingabiosensorbasedonimagingellipsometry AT dongxianzhi detectionofcytomegalovirusantibodiesusingabiosensorbasedonimagingellipsometry AT wangchunhua detectionofcytomegalovirusantibodiesusingabiosensorbasedonimagingellipsometry AT zhuwei detectionofcytomegalovirusantibodiesusingabiosensorbasedonimagingellipsometry |