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Determinants of testosterone levels in human male obesity

Testosterone (T) levels are decreased in obese men, but the underlying causes are incompletely understood. Our objective was to explore the relation between low (free) T levels and male obesity, by evaluating metabolic parameters, subcutaneous adipose tissue (SAT) aromatase expression, and parameter...

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Autores principales: Bekaert, Marlies, Van Nieuwenhove, Yves, Calders, Patrick, Cuvelier, Claude A., Batens, Arsène-Hélène, Kaufman, Jean-Marc, Ouwens, D. Margriet, Ruige, Johannes B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546699/
https://www.ncbi.nlm.nih.gov/pubmed/25771885
http://dx.doi.org/10.1007/s12020-015-0563-4
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author Bekaert, Marlies
Van Nieuwenhove, Yves
Calders, Patrick
Cuvelier, Claude A.
Batens, Arsène-Hélène
Kaufman, Jean-Marc
Ouwens, D. Margriet
Ruige, Johannes B.
author_facet Bekaert, Marlies
Van Nieuwenhove, Yves
Calders, Patrick
Cuvelier, Claude A.
Batens, Arsène-Hélène
Kaufman, Jean-Marc
Ouwens, D. Margriet
Ruige, Johannes B.
author_sort Bekaert, Marlies
collection PubMed
description Testosterone (T) levels are decreased in obese men, but the underlying causes are incompletely understood. Our objective was to explore the relation between low (free) T levels and male obesity, by evaluating metabolic parameters, subcutaneous adipose tissue (SAT) aromatase expression, and parameters of the hypothalamic–pituitary–gonadal axis. We recruited 57 morbidly obese men [33 had type 2 diabetes (DM2)] and 25 normal-weight men undergoing abdominal surgery. Fourteen obese men also attended a follow-up, 2 years after gastric bypass surgery (GBS). Circulating T levels were quantified by LC–MS/MS, whereas free T levels were measured using serum equilibrium dialysis and sex hormone-binding globulin, luteinizing hormone, and follicle-stimulating hormone by immunoassay. SAT biopsies were used to determine adipocyte cell size and aromatase expression by real-time PCR. Total and free T levels were decreased in obese males versus controls, with a further decrease in obese men with DM2 versus obese men without DM2. There were no differences in aromatase expression among the study groups, and sex steroids did not correlate with aromatase expression. Pearson analysis revealed an inverse association between (free) T and SAT cell size, triglycerides, and HOMA-IR. Multivariate analysis confirmed the inverse association between (free) T and SAT cell size (β = −0.321, P = 0.037 and β = −0.441, P = 0.011, respectively), independent of age, triglycerides, HOMA-IR, obesity, or diabetes. T levels were normalized 2 years after GBS. These data suggest that SAT cell size rather than SAT aromatase expression or parameters of the hypothalamic–pituitary–gonadal axis is related to low T in male obesity, which points to adipose cell size-related metabolic changes as a major trigger in decreased T levels.
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spelling pubmed-45466992015-08-25 Determinants of testosterone levels in human male obesity Bekaert, Marlies Van Nieuwenhove, Yves Calders, Patrick Cuvelier, Claude A. Batens, Arsène-Hélène Kaufman, Jean-Marc Ouwens, D. Margriet Ruige, Johannes B. Endocrine Original Article Testosterone (T) levels are decreased in obese men, but the underlying causes are incompletely understood. Our objective was to explore the relation between low (free) T levels and male obesity, by evaluating metabolic parameters, subcutaneous adipose tissue (SAT) aromatase expression, and parameters of the hypothalamic–pituitary–gonadal axis. We recruited 57 morbidly obese men [33 had type 2 diabetes (DM2)] and 25 normal-weight men undergoing abdominal surgery. Fourteen obese men also attended a follow-up, 2 years after gastric bypass surgery (GBS). Circulating T levels were quantified by LC–MS/MS, whereas free T levels were measured using serum equilibrium dialysis and sex hormone-binding globulin, luteinizing hormone, and follicle-stimulating hormone by immunoassay. SAT biopsies were used to determine adipocyte cell size and aromatase expression by real-time PCR. Total and free T levels were decreased in obese males versus controls, with a further decrease in obese men with DM2 versus obese men without DM2. There were no differences in aromatase expression among the study groups, and sex steroids did not correlate with aromatase expression. Pearson analysis revealed an inverse association between (free) T and SAT cell size, triglycerides, and HOMA-IR. Multivariate analysis confirmed the inverse association between (free) T and SAT cell size (β = −0.321, P = 0.037 and β = −0.441, P = 0.011, respectively), independent of age, triglycerides, HOMA-IR, obesity, or diabetes. T levels were normalized 2 years after GBS. These data suggest that SAT cell size rather than SAT aromatase expression or parameters of the hypothalamic–pituitary–gonadal axis is related to low T in male obesity, which points to adipose cell size-related metabolic changes as a major trigger in decreased T levels. Springer US 2015-03-13 2015 /pmc/articles/PMC4546699/ /pubmed/25771885 http://dx.doi.org/10.1007/s12020-015-0563-4 Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Bekaert, Marlies
Van Nieuwenhove, Yves
Calders, Patrick
Cuvelier, Claude A.
Batens, Arsène-Hélène
Kaufman, Jean-Marc
Ouwens, D. Margriet
Ruige, Johannes B.
Determinants of testosterone levels in human male obesity
title Determinants of testosterone levels in human male obesity
title_full Determinants of testosterone levels in human male obesity
title_fullStr Determinants of testosterone levels in human male obesity
title_full_unstemmed Determinants of testosterone levels in human male obesity
title_short Determinants of testosterone levels in human male obesity
title_sort determinants of testosterone levels in human male obesity
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546699/
https://www.ncbi.nlm.nih.gov/pubmed/25771885
http://dx.doi.org/10.1007/s12020-015-0563-4
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