Cargando…

Genetic integrity of four species of Leptidea (Pieridae, Lepidoptera) as sampled in sympatry in West Siberia

Abstract. In southern West Siberia, as many as four Leptidea Billberg, 1820 species are present sympatrically: Leptidea amurensis (Ménétriés, 1859), Leptidea morsei (Ménétriés, 1859), Leptidea sinapis (Linnaeus, 1758) and Leptidea juvernica Williams, 1946. The two latter were recently recognised as...

Descripción completa

Detalles Bibliográficos
Autores principales: Solovyev, Vladimir I., Ilinsky, Yury, Kosterin, Oleg E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547032/
https://www.ncbi.nlm.nih.gov/pubmed/26312129
http://dx.doi.org/10.3897/CompCytogen.v9i3.4636
_version_ 1782387021762789376
author Solovyev, Vladimir I.
Ilinsky, Yury
Kosterin, Oleg E.
author_facet Solovyev, Vladimir I.
Ilinsky, Yury
Kosterin, Oleg E.
author_sort Solovyev, Vladimir I.
collection PubMed
description Abstract. In southern West Siberia, as many as four Leptidea Billberg, 1820 species are present sympatrically: Leptidea amurensis (Ménétriés, 1859), Leptidea morsei (Ménétriés, 1859), Leptidea sinapis (Linnaeus, 1758) and Leptidea juvernica Williams, 1946. The two latter were recently recognised as nearly sibling species on morphological and molecular characters. Specimens intermediate as to their subtle diagnostic characters occurring in West Siberia and elsewhere were interpreted as resulted from limited introgression. This supposition was tested via populational morphological and molecular analysis of spring brood specimens of all the four species taken from a limited (4.5 × 0.2 km) area in the suburbs of Novosibirsk. The samples were analysed with respect to the genitalic morphology, external characters, three nuclear (CAD, H1 gene and ITS2) and one mitochondrial (COI) molecular markers, infection of the intracellular maternally inherited bacterial symbiont Wolbachia Hertig, 1836 and its wsp gene coding for a hypervariable surface protein. Interspecific variation of the nuclear CAD and ITS2 sequences and the mitochondrial COI gene in Leptidea sinapis and Leptidea juvernica turned out concordant. The absence of molecular evidence of introgression suggests genetic integrity of these two species and allows their reliable identification by molecular characters. The genitalic (lengths of the saccus and valva) and external characters (wing pattern) of males overlap in Leptidea sinapis and Leptidea juvernica, as identified by molecular markers and thus are not so helpful in actual species identification. Only the ductus bursae length showed no overlap and can be used for identification of females. The histone H1 gene appeared five times less variable over the four studied species than COI, and found to be identical in species Leptidea sinapis and Leptidea juvernica. Wolbachia infection was found in all studied species. We identified three wsp variants of Wolbachia: 1) wsp-10 allele in Leptidea amurensis, Leptidea sinapis, Leptidea juvernica; 2) a very similar wsp-687 allele in Leptidea sinapis; and 3) wsp-688, highly divergent to the previous ones, in Leptidea morsei.
format Online
Article
Text
id pubmed-4547032
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Pensoft Publishers
record_format MEDLINE/PubMed
spelling pubmed-45470322015-08-26 Genetic integrity of four species of Leptidea (Pieridae, Lepidoptera) as sampled in sympatry in West Siberia Solovyev, Vladimir I. Ilinsky, Yury Kosterin, Oleg E. Comp Cytogenet Research Articles Abstract. In southern West Siberia, as many as four Leptidea Billberg, 1820 species are present sympatrically: Leptidea amurensis (Ménétriés, 1859), Leptidea morsei (Ménétriés, 1859), Leptidea sinapis (Linnaeus, 1758) and Leptidea juvernica Williams, 1946. The two latter were recently recognised as nearly sibling species on morphological and molecular characters. Specimens intermediate as to their subtle diagnostic characters occurring in West Siberia and elsewhere were interpreted as resulted from limited introgression. This supposition was tested via populational morphological and molecular analysis of spring brood specimens of all the four species taken from a limited (4.5 × 0.2 km) area in the suburbs of Novosibirsk. The samples were analysed with respect to the genitalic morphology, external characters, three nuclear (CAD, H1 gene and ITS2) and one mitochondrial (COI) molecular markers, infection of the intracellular maternally inherited bacterial symbiont Wolbachia Hertig, 1836 and its wsp gene coding for a hypervariable surface protein. Interspecific variation of the nuclear CAD and ITS2 sequences and the mitochondrial COI gene in Leptidea sinapis and Leptidea juvernica turned out concordant. The absence of molecular evidence of introgression suggests genetic integrity of these two species and allows their reliable identification by molecular characters. The genitalic (lengths of the saccus and valva) and external characters (wing pattern) of males overlap in Leptidea sinapis and Leptidea juvernica, as identified by molecular markers and thus are not so helpful in actual species identification. Only the ductus bursae length showed no overlap and can be used for identification of females. The histone H1 gene appeared five times less variable over the four studied species than COI, and found to be identical in species Leptidea sinapis and Leptidea juvernica. Wolbachia infection was found in all studied species. We identified three wsp variants of Wolbachia: 1) wsp-10 allele in Leptidea amurensis, Leptidea sinapis, Leptidea juvernica; 2) a very similar wsp-687 allele in Leptidea sinapis; and 3) wsp-688, highly divergent to the previous ones, in Leptidea morsei. Pensoft Publishers 2015-06-23 /pmc/articles/PMC4547032/ /pubmed/26312129 http://dx.doi.org/10.3897/CompCytogen.v9i3.4636 Text en Vladimir I. Solovyev, Yury Ilinsky, Oleg E. Kosterin http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Articles
Solovyev, Vladimir I.
Ilinsky, Yury
Kosterin, Oleg E.
Genetic integrity of four species of Leptidea (Pieridae, Lepidoptera) as sampled in sympatry in West Siberia
title Genetic integrity of four species of Leptidea (Pieridae, Lepidoptera) as sampled in sympatry in West Siberia
title_full Genetic integrity of four species of Leptidea (Pieridae, Lepidoptera) as sampled in sympatry in West Siberia
title_fullStr Genetic integrity of four species of Leptidea (Pieridae, Lepidoptera) as sampled in sympatry in West Siberia
title_full_unstemmed Genetic integrity of four species of Leptidea (Pieridae, Lepidoptera) as sampled in sympatry in West Siberia
title_short Genetic integrity of four species of Leptidea (Pieridae, Lepidoptera) as sampled in sympatry in West Siberia
title_sort genetic integrity of four species of leptidea (pieridae, lepidoptera) as sampled in sympatry in west siberia
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547032/
https://www.ncbi.nlm.nih.gov/pubmed/26312129
http://dx.doi.org/10.3897/CompCytogen.v9i3.4636
work_keys_str_mv AT solovyevvladimiri geneticintegrityoffourspeciesofleptideapieridaelepidopteraassampledinsympatryinwestsiberia
AT ilinskyyury geneticintegrityoffourspeciesofleptideapieridaelepidopteraassampledinsympatryinwestsiberia
AT kosterinolege geneticintegrityoffourspeciesofleptideapieridaelepidopteraassampledinsympatryinwestsiberia