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Variation in the nuclear effects of infection by different human rhinovirus serotypes
Human rhinovirus (HRV) is a positive sense RNA virus, which, despite replicating in the cytoplasm, has a significant impact on nuclear transport and nuclear localization of host proteins. A number of studies have identified differences between HRV serotypes, with respect to host response, protease a...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547043/ https://www.ncbi.nlm.nih.gov/pubmed/26379650 http://dx.doi.org/10.3389/fmicb.2015.00875 |
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author | Walker, Erin J. Jensen, Lora M. Croft, Sarah Ghildyal, Reena |
author_facet | Walker, Erin J. Jensen, Lora M. Croft, Sarah Ghildyal, Reena |
author_sort | Walker, Erin J. |
collection | PubMed |
description | Human rhinovirus (HRV) is a positive sense RNA virus, which, despite replicating in the cytoplasm, has a significant impact on nuclear transport and nuclear localization of host proteins. A number of studies have identified differences between HRV serotypes, with respect to host response, protease activity and replicative ability. Here we report the sero-specific effects of two group-A HRV serotypes, the minor group HRV2 and the major group HRV16, on nuclear transport and nuclear protein localization. Using Western analysis, immunofluorescence and real time PCR, we show that HRV2 replicates at a faster rate than HRV16, which correlates with earlier production of viral proteases and disruption of host nuclear transport. There is significant variation in the nuclear effects of different rhinovirus species, which in turn may impact disease progression and patient response. |
format | Online Article Text |
id | pubmed-4547043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-45470432015-09-14 Variation in the nuclear effects of infection by different human rhinovirus serotypes Walker, Erin J. Jensen, Lora M. Croft, Sarah Ghildyal, Reena Front Microbiol Microbiology Human rhinovirus (HRV) is a positive sense RNA virus, which, despite replicating in the cytoplasm, has a significant impact on nuclear transport and nuclear localization of host proteins. A number of studies have identified differences between HRV serotypes, with respect to host response, protease activity and replicative ability. Here we report the sero-specific effects of two group-A HRV serotypes, the minor group HRV2 and the major group HRV16, on nuclear transport and nuclear protein localization. Using Western analysis, immunofluorescence and real time PCR, we show that HRV2 replicates at a faster rate than HRV16, which correlates with earlier production of viral proteases and disruption of host nuclear transport. There is significant variation in the nuclear effects of different rhinovirus species, which in turn may impact disease progression and patient response. Frontiers Media S.A. 2015-08-24 /pmc/articles/PMC4547043/ /pubmed/26379650 http://dx.doi.org/10.3389/fmicb.2015.00875 Text en Copyright © 2015 Walker, Jensen, Croft and Ghildyal. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Walker, Erin J. Jensen, Lora M. Croft, Sarah Ghildyal, Reena Variation in the nuclear effects of infection by different human rhinovirus serotypes |
title | Variation in the nuclear effects of infection by different human rhinovirus serotypes |
title_full | Variation in the nuclear effects of infection by different human rhinovirus serotypes |
title_fullStr | Variation in the nuclear effects of infection by different human rhinovirus serotypes |
title_full_unstemmed | Variation in the nuclear effects of infection by different human rhinovirus serotypes |
title_short | Variation in the nuclear effects of infection by different human rhinovirus serotypes |
title_sort | variation in the nuclear effects of infection by different human rhinovirus serotypes |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547043/ https://www.ncbi.nlm.nih.gov/pubmed/26379650 http://dx.doi.org/10.3389/fmicb.2015.00875 |
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