Imaging real-time HIV-1 virion fusion with FRET-based biosensors

We have produced a novel, simple and rapid method utilising genetically encodable FRET-based biosensors to permit the detection of HIV-1 virion fusion in living cells. These biosensors show high sensitivity both spatially and temporally, and allow the real-time recovery of HIV-1 fusion kinetics in b...

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Detalles Bibliográficos
Autores principales: Jones, Daniel M., Padilla-Parra, Sergi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547133/
https://www.ncbi.nlm.nih.gov/pubmed/26300212
http://dx.doi.org/10.1038/srep13449
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author Jones, Daniel M.
Padilla-Parra, Sergi
author_facet Jones, Daniel M.
Padilla-Parra, Sergi
author_sort Jones, Daniel M.
collection PubMed
description We have produced a novel, simple and rapid method utilising genetically encodable FRET-based biosensors to permit the detection of HIV-1 virion fusion in living cells. These biosensors show high sensitivity both spatially and temporally, and allow the real-time recovery of HIV-1 fusion kinetics in both single cells and cell populations simultaneously.
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spelling pubmed-45471332015-08-26 Imaging real-time HIV-1 virion fusion with FRET-based biosensors Jones, Daniel M. Padilla-Parra, Sergi Sci Rep Article We have produced a novel, simple and rapid method utilising genetically encodable FRET-based biosensors to permit the detection of HIV-1 virion fusion in living cells. These biosensors show high sensitivity both spatially and temporally, and allow the real-time recovery of HIV-1 fusion kinetics in both single cells and cell populations simultaneously. Nature Publishing Group 2015-08-24 /pmc/articles/PMC4547133/ /pubmed/26300212 http://dx.doi.org/10.1038/srep13449 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Jones, Daniel M.
Padilla-Parra, Sergi
Imaging real-time HIV-1 virion fusion with FRET-based biosensors
title Imaging real-time HIV-1 virion fusion with FRET-based biosensors
title_full Imaging real-time HIV-1 virion fusion with FRET-based biosensors
title_fullStr Imaging real-time HIV-1 virion fusion with FRET-based biosensors
title_full_unstemmed Imaging real-time HIV-1 virion fusion with FRET-based biosensors
title_short Imaging real-time HIV-1 virion fusion with FRET-based biosensors
title_sort imaging real-time hiv-1 virion fusion with fret-based biosensors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547133/
https://www.ncbi.nlm.nih.gov/pubmed/26300212
http://dx.doi.org/10.1038/srep13449
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