Plasmodium falciparum genetic crosses in a humanized mouse model

Genetic crosses of phenotypically distinct strains of the human malaria parasite Plasmodium falciparum are a powerful tool for identifying genes controlling drug resistance and other key phenotypes. Previous studies relied on the isolation of recombinant parasites from splenectomized chimpanzees, a...

Descripción completa

Detalles Bibliográficos
Autores principales: Vaughan, Ashley M., Pinapati, Richard S., Cheeseman, Ian H., Camargo, Nelly, Fishbaugher, Matthew, Checkley, Lisa A., Nair, Shalini, Hutyra, Carolyn A., Nosten, François H., Anderson, Timothy J. C., Ferdig, Michael T., Kappe, Stefan H. I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547688/
https://www.ncbi.nlm.nih.gov/pubmed/26030447
http://dx.doi.org/10.1038/nmeth.3432
_version_ 1782387092995702784
author Vaughan, Ashley M.
Pinapati, Richard S.
Cheeseman, Ian H.
Camargo, Nelly
Fishbaugher, Matthew
Checkley, Lisa A.
Nair, Shalini
Hutyra, Carolyn A.
Nosten, François H.
Anderson, Timothy J. C.
Ferdig, Michael T.
Kappe, Stefan H. I.
author_facet Vaughan, Ashley M.
Pinapati, Richard S.
Cheeseman, Ian H.
Camargo, Nelly
Fishbaugher, Matthew
Checkley, Lisa A.
Nair, Shalini
Hutyra, Carolyn A.
Nosten, François H.
Anderson, Timothy J. C.
Ferdig, Michael T.
Kappe, Stefan H. I.
author_sort Vaughan, Ashley M.
collection PubMed
description Genetic crosses of phenotypically distinct strains of the human malaria parasite Plasmodium falciparum are a powerful tool for identifying genes controlling drug resistance and other key phenotypes. Previous studies relied on the isolation of recombinant parasites from splenectomized chimpanzees, a research avenue that is no longer available. Here, we demonstrate that human-liver chimeric mice support recovery of recombinant progeny for the identification of genetic determinants of parasite traits and adaptations.
format Online
Article
Text
id pubmed-4547688
institution National Center for Biotechnology Information
language English
publishDate 2015
record_format MEDLINE/PubMed
spelling pubmed-45476882016-01-01 Plasmodium falciparum genetic crosses in a humanized mouse model Vaughan, Ashley M. Pinapati, Richard S. Cheeseman, Ian H. Camargo, Nelly Fishbaugher, Matthew Checkley, Lisa A. Nair, Shalini Hutyra, Carolyn A. Nosten, François H. Anderson, Timothy J. C. Ferdig, Michael T. Kappe, Stefan H. I. Nat Methods Article Genetic crosses of phenotypically distinct strains of the human malaria parasite Plasmodium falciparum are a powerful tool for identifying genes controlling drug resistance and other key phenotypes. Previous studies relied on the isolation of recombinant parasites from splenectomized chimpanzees, a research avenue that is no longer available. Here, we demonstrate that human-liver chimeric mice support recovery of recombinant progeny for the identification of genetic determinants of parasite traits and adaptations. 2015-06-01 2015-07 /pmc/articles/PMC4547688/ /pubmed/26030447 http://dx.doi.org/10.1038/nmeth.3432 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Vaughan, Ashley M.
Pinapati, Richard S.
Cheeseman, Ian H.
Camargo, Nelly
Fishbaugher, Matthew
Checkley, Lisa A.
Nair, Shalini
Hutyra, Carolyn A.
Nosten, François H.
Anderson, Timothy J. C.
Ferdig, Michael T.
Kappe, Stefan H. I.
Plasmodium falciparum genetic crosses in a humanized mouse model
title Plasmodium falciparum genetic crosses in a humanized mouse model
title_full Plasmodium falciparum genetic crosses in a humanized mouse model
title_fullStr Plasmodium falciparum genetic crosses in a humanized mouse model
title_full_unstemmed Plasmodium falciparum genetic crosses in a humanized mouse model
title_short Plasmodium falciparum genetic crosses in a humanized mouse model
title_sort plasmodium falciparum genetic crosses in a humanized mouse model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547688/
https://www.ncbi.nlm.nih.gov/pubmed/26030447
http://dx.doi.org/10.1038/nmeth.3432
work_keys_str_mv AT vaughanashleym plasmodiumfalciparumgeneticcrossesinahumanizedmousemodel
AT pinapatirichards plasmodiumfalciparumgeneticcrossesinahumanizedmousemodel
AT cheesemanianh plasmodiumfalciparumgeneticcrossesinahumanizedmousemodel
AT camargonelly plasmodiumfalciparumgeneticcrossesinahumanizedmousemodel
AT fishbaughermatthew plasmodiumfalciparumgeneticcrossesinahumanizedmousemodel
AT checkleylisaa plasmodiumfalciparumgeneticcrossesinahumanizedmousemodel
AT nairshalini plasmodiumfalciparumgeneticcrossesinahumanizedmousemodel
AT hutyracarolyna plasmodiumfalciparumgeneticcrossesinahumanizedmousemodel
AT nostenfrancoish plasmodiumfalciparumgeneticcrossesinahumanizedmousemodel
AT andersontimothyjc plasmodiumfalciparumgeneticcrossesinahumanizedmousemodel
AT ferdigmichaelt plasmodiumfalciparumgeneticcrossesinahumanizedmousemodel
AT kappestefanhi plasmodiumfalciparumgeneticcrossesinahumanizedmousemodel

Ejemplares similares