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Effects of four-week feed restriction on toxicological parameters in beagle dogs

This study was conducted to examine any changes caused by feed restriction in dogs to contribute to safety evaluation in toxicity studies. Two male 7-month-old beagle dogs/group were fed 300 (control), 150 (50% of control), or 70 g/animal of diet daily (23% of control) for 4 weeks. Effects of feed r...

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Autores principales: TAKAMATSU, Kazuhiko, YAMASHITA, Hiroyuki, SATAKE, Shigeru, KAZUSA, Katsuyuki, TABATA, Hajime, NISHIKATA, Takahito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548000/
https://www.ncbi.nlm.nih.gov/pubmed/25818481
http://dx.doi.org/10.1538/expanim.14-0098
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author TAKAMATSU, Kazuhiko
YAMASHITA, Hiroyuki
SATAKE, Shigeru
KAZUSA, Katsuyuki
TABATA, Hajime
NISHIKATA, Takahito
author_facet TAKAMATSU, Kazuhiko
YAMASHITA, Hiroyuki
SATAKE, Shigeru
KAZUSA, Katsuyuki
TABATA, Hajime
NISHIKATA, Takahito
author_sort TAKAMATSU, Kazuhiko
collection PubMed
description This study was conducted to examine any changes caused by feed restriction in dogs to contribute to safety evaluation in toxicity studies. Two male 7-month-old beagle dogs/group were fed 300 (control), 150 (50% of control), or 70 g/animal of diet daily (23% of control) for 4 weeks. Effects of feed restriction, except for clinical signs, were noted depending on the feed dosage in almost all examinations. The principal outcomes were: decreased body weight and water consumption, ECG changes (decreased heart rate and prolonged QTc), and hematopoietic and lymphopoietic suppression (decreased reticulocyte ratio or white blood cell count in hematology, decreased nucleated cell count in bone marrow, decreased erythroid parameters in myelography, and hypocellularity of bone marrow and thymic atrophy in histopathology). In addition, some changes were noted in urinalysis (decreased urine volume and sodium and potassium excretion), blood chemistry (decreased ALP and inorganic phosphorus and increased creatinine), organ weights, and gastric histopathology. These results provide important reference data for distinguishing the primary effects of test compounds from secondary effects of decreased food consumption in toxicity studies in beagle dogs.
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spelling pubmed-45480002015-08-26 Effects of four-week feed restriction on toxicological parameters in beagle dogs TAKAMATSU, Kazuhiko YAMASHITA, Hiroyuki SATAKE, Shigeru KAZUSA, Katsuyuki TABATA, Hajime NISHIKATA, Takahito Exp Anim Original This study was conducted to examine any changes caused by feed restriction in dogs to contribute to safety evaluation in toxicity studies. Two male 7-month-old beagle dogs/group were fed 300 (control), 150 (50% of control), or 70 g/animal of diet daily (23% of control) for 4 weeks. Effects of feed restriction, except for clinical signs, were noted depending on the feed dosage in almost all examinations. The principal outcomes were: decreased body weight and water consumption, ECG changes (decreased heart rate and prolonged QTc), and hematopoietic and lymphopoietic suppression (decreased reticulocyte ratio or white blood cell count in hematology, decreased nucleated cell count in bone marrow, decreased erythroid parameters in myelography, and hypocellularity of bone marrow and thymic atrophy in histopathology). In addition, some changes were noted in urinalysis (decreased urine volume and sodium and potassium excretion), blood chemistry (decreased ALP and inorganic phosphorus and increased creatinine), organ weights, and gastric histopathology. These results provide important reference data for distinguishing the primary effects of test compounds from secondary effects of decreased food consumption in toxicity studies in beagle dogs. Japanese Association for Laboratory Animal Science 2015-03-30 2015 /pmc/articles/PMC4548000/ /pubmed/25818481 http://dx.doi.org/10.1538/expanim.14-0098 Text en ©2015 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original
TAKAMATSU, Kazuhiko
YAMASHITA, Hiroyuki
SATAKE, Shigeru
KAZUSA, Katsuyuki
TABATA, Hajime
NISHIKATA, Takahito
Effects of four-week feed restriction on toxicological parameters in beagle dogs
title Effects of four-week feed restriction on toxicological parameters in beagle dogs
title_full Effects of four-week feed restriction on toxicological parameters in beagle dogs
title_fullStr Effects of four-week feed restriction on toxicological parameters in beagle dogs
title_full_unstemmed Effects of four-week feed restriction on toxicological parameters in beagle dogs
title_short Effects of four-week feed restriction on toxicological parameters in beagle dogs
title_sort effects of four-week feed restriction on toxicological parameters in beagle dogs
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548000/
https://www.ncbi.nlm.nih.gov/pubmed/25818481
http://dx.doi.org/10.1538/expanim.14-0098
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