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Gestational diabetes mellitus is associated with increased leukocyte peroxisome proliferator-activated receptor γ expression
INTRODUCTION: Peroxisome proliferator-activated receptor γ (PPARγ) is a ligand-activated transcription factor of the nuclear receptor superfamily that is involved in lipid and carbohydrate metabolism as well as inflammation; thereby it participates in metabolic diseases including diabetes. Although...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548020/ https://www.ncbi.nlm.nih.gov/pubmed/26322090 http://dx.doi.org/10.5114/aoms.2015.47692 |
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author | Wójcik, Marzena Mac-Marcjanek, Katarzyna Nadel, Iwona Woźniak, Lucyna Cypryk, Katarzyna |
author_facet | Wójcik, Marzena Mac-Marcjanek, Katarzyna Nadel, Iwona Woźniak, Lucyna Cypryk, Katarzyna |
author_sort | Wójcik, Marzena |
collection | PubMed |
description | INTRODUCTION: Peroxisome proliferator-activated receptor γ (PPARγ) is a ligand-activated transcription factor of the nuclear receptor superfamily that is involved in lipid and carbohydrate metabolism as well as inflammation; thereby it participates in metabolic diseases including diabetes. Although PPARγ expression has been observed in different tissues of diabetic patients, its level in leukocytes from subjects affected by gestational diabetes mellitus (GDM) has not yet been reported. This study aimed to investigate leukocyte PPARG expression in GDM patients at 24–33 weeks of gestation and, in turn, to correlate these alterations with anthropometric and metabolic parameters of patients. MATERIAL AND METHODS: Leukocytes were isolated from the blood of normal glucose tolerant (NGT; n = 34) and GDM (n = 77) pregnant women between 24 and 33 weeks of gestation. Leukocyte PPARG mRNA expression was determined by semi-quantitative polymerase chain reaction. Univariate correlation analysis was performed to investigate associations between PPARG expression and clinical characteristics of patients. RESULTS: Leukocyte PPARG mRNA level was significantly higher in GDM than NGT women (p < 0.05). In the whole study group, PPARG expression positively correlated with plasma glucose concentrations at 1 h (r = 0.222, p = 0.049) and 2 h (r = 0.315, p = 0.020) of 75 g oral glucose tolerance test (OGTT), and negatively correlated with plasma HDL cholesterol concentration (r = -0.351, p = 0.010). CONCLUSIONS: The correlation between leukocyte PPARG overexpression and hyperglycaemia suggests that PPARG mRNA expression in these cells might be up-regulated in high-glucose conditions in GDM patients at 24–33 weeks of gestation. |
format | Online Article Text |
id | pubmed-4548020 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-45480202015-08-28 Gestational diabetes mellitus is associated with increased leukocyte peroxisome proliferator-activated receptor γ expression Wójcik, Marzena Mac-Marcjanek, Katarzyna Nadel, Iwona Woźniak, Lucyna Cypryk, Katarzyna Arch Med Sci Clinical Research INTRODUCTION: Peroxisome proliferator-activated receptor γ (PPARγ) is a ligand-activated transcription factor of the nuclear receptor superfamily that is involved in lipid and carbohydrate metabolism as well as inflammation; thereby it participates in metabolic diseases including diabetes. Although PPARγ expression has been observed in different tissues of diabetic patients, its level in leukocytes from subjects affected by gestational diabetes mellitus (GDM) has not yet been reported. This study aimed to investigate leukocyte PPARG expression in GDM patients at 24–33 weeks of gestation and, in turn, to correlate these alterations with anthropometric and metabolic parameters of patients. MATERIAL AND METHODS: Leukocytes were isolated from the blood of normal glucose tolerant (NGT; n = 34) and GDM (n = 77) pregnant women between 24 and 33 weeks of gestation. Leukocyte PPARG mRNA expression was determined by semi-quantitative polymerase chain reaction. Univariate correlation analysis was performed to investigate associations between PPARG expression and clinical characteristics of patients. RESULTS: Leukocyte PPARG mRNA level was significantly higher in GDM than NGT women (p < 0.05). In the whole study group, PPARG expression positively correlated with plasma glucose concentrations at 1 h (r = 0.222, p = 0.049) and 2 h (r = 0.315, p = 0.020) of 75 g oral glucose tolerance test (OGTT), and negatively correlated with plasma HDL cholesterol concentration (r = -0.351, p = 0.010). CONCLUSIONS: The correlation between leukocyte PPARG overexpression and hyperglycaemia suggests that PPARG mRNA expression in these cells might be up-regulated in high-glucose conditions in GDM patients at 24–33 weeks of gestation. Termedia Publishing House 2015-01-14 2015-08-12 /pmc/articles/PMC4548020/ /pubmed/26322090 http://dx.doi.org/10.5114/aoms.2015.47692 Text en Copyright © 2015 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Research Wójcik, Marzena Mac-Marcjanek, Katarzyna Nadel, Iwona Woźniak, Lucyna Cypryk, Katarzyna Gestational diabetes mellitus is associated with increased leukocyte peroxisome proliferator-activated receptor γ expression |
title | Gestational diabetes mellitus is associated with increased leukocyte peroxisome proliferator-activated receptor γ expression |
title_full | Gestational diabetes mellitus is associated with increased leukocyte peroxisome proliferator-activated receptor γ expression |
title_fullStr | Gestational diabetes mellitus is associated with increased leukocyte peroxisome proliferator-activated receptor γ expression |
title_full_unstemmed | Gestational diabetes mellitus is associated with increased leukocyte peroxisome proliferator-activated receptor γ expression |
title_short | Gestational diabetes mellitus is associated with increased leukocyte peroxisome proliferator-activated receptor γ expression |
title_sort | gestational diabetes mellitus is associated with increased leukocyte peroxisome proliferator-activated receptor γ expression |
topic | Clinical Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548020/ https://www.ncbi.nlm.nih.gov/pubmed/26322090 http://dx.doi.org/10.5114/aoms.2015.47692 |
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