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Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark of Curatella americana L. (Dilleniaceae) against Candida Species

The ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the Brazilian cerrado, in which crude extract showed antifungal activity in a preliminary study. In this work, the antifungal and cytotoxic properties of the crude extract, fractions, and isolated compounds from C. a...

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Autores principales: Mendes de Toledo, Cleyton Eduardo, Santos, Patrícia Regina, Palazzo de Mello, João Carlos, Dias Filho, Benedito Prado, Nakamura, Celso Vataru, Ueda-Nakamura, Tânia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548135/
https://www.ncbi.nlm.nih.gov/pubmed/26347790
http://dx.doi.org/10.1155/2015/673962
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author Mendes de Toledo, Cleyton Eduardo
Santos, Patrícia Regina
Palazzo de Mello, João Carlos
Dias Filho, Benedito Prado
Nakamura, Celso Vataru
Ueda-Nakamura, Tânia
author_facet Mendes de Toledo, Cleyton Eduardo
Santos, Patrícia Regina
Palazzo de Mello, João Carlos
Dias Filho, Benedito Prado
Nakamura, Celso Vataru
Ueda-Nakamura, Tânia
author_sort Mendes de Toledo, Cleyton Eduardo
collection PubMed
description The ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the Brazilian cerrado, in which crude extract showed antifungal activity in a preliminary study. In this work, the antifungal and cytotoxic properties of the crude extract, fractions, and isolated compounds from C. americana were evaluated against the standard yeast strains Candida albicans, C. tropicalis, and C. parapsilosis, clinical isolates, and fluconazole-resistant strains. The combinatory effects between subfractions and isolated compounds and effects on cell morphology, virulence factors, and exogenous ergosterol were also evaluated. The MIC obtained against the Candida species including fluconazole-resistant strain ranged from 15.3 to 31.3 µg/mL for crude extract, 3.9 to 15.6 µg/mL for ethyl acetate fraction, and 7.8 to 31.3 µg/mL for subfractions. The isolated compounds identified as 4′-O-methyl-catechin, epicatechin-3-O-gallate, and 4′-O-methyl-catechin-3-O-gallate showed lower antifungal activity than the crude extract and fractions (MIC ranging from 31.3 to 125.0 µg/mL). The addition of exogenous ergosterol to yeast culture did not interfere in the antifungal activity of the extract and its fractions. Synergistic antifungal activity was observed between subfractions and isolated compounds. The effects on virulence factors and the different mechanisms of action compared to fluconazole and nystatin suggest that this ethnomedicinal plant may be an effective alternative treatment for candidiasis.
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spelling pubmed-45481352015-09-07 Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark of Curatella americana L. (Dilleniaceae) against Candida Species Mendes de Toledo, Cleyton Eduardo Santos, Patrícia Regina Palazzo de Mello, João Carlos Dias Filho, Benedito Prado Nakamura, Celso Vataru Ueda-Nakamura, Tânia Evid Based Complement Alternat Med Research Article The ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the Brazilian cerrado, in which crude extract showed antifungal activity in a preliminary study. In this work, the antifungal and cytotoxic properties of the crude extract, fractions, and isolated compounds from C. americana were evaluated against the standard yeast strains Candida albicans, C. tropicalis, and C. parapsilosis, clinical isolates, and fluconazole-resistant strains. The combinatory effects between subfractions and isolated compounds and effects on cell morphology, virulence factors, and exogenous ergosterol were also evaluated. The MIC obtained against the Candida species including fluconazole-resistant strain ranged from 15.3 to 31.3 µg/mL for crude extract, 3.9 to 15.6 µg/mL for ethyl acetate fraction, and 7.8 to 31.3 µg/mL for subfractions. The isolated compounds identified as 4′-O-methyl-catechin, epicatechin-3-O-gallate, and 4′-O-methyl-catechin-3-O-gallate showed lower antifungal activity than the crude extract and fractions (MIC ranging from 31.3 to 125.0 µg/mL). The addition of exogenous ergosterol to yeast culture did not interfere in the antifungal activity of the extract and its fractions. Synergistic antifungal activity was observed between subfractions and isolated compounds. The effects on virulence factors and the different mechanisms of action compared to fluconazole and nystatin suggest that this ethnomedicinal plant may be an effective alternative treatment for candidiasis. Hindawi Publishing Corporation 2015 2015-08-11 /pmc/articles/PMC4548135/ /pubmed/26347790 http://dx.doi.org/10.1155/2015/673962 Text en Copyright © 2015 Cleyton Eduardo Mendes de Toledo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mendes de Toledo, Cleyton Eduardo
Santos, Patrícia Regina
Palazzo de Mello, João Carlos
Dias Filho, Benedito Prado
Nakamura, Celso Vataru
Ueda-Nakamura, Tânia
Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark of Curatella americana L. (Dilleniaceae) against Candida Species
title Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark of Curatella americana L. (Dilleniaceae) against Candida Species
title_full Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark of Curatella americana L. (Dilleniaceae) against Candida Species
title_fullStr Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark of Curatella americana L. (Dilleniaceae) against Candida Species
title_full_unstemmed Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark of Curatella americana L. (Dilleniaceae) against Candida Species
title_short Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark of Curatella americana L. (Dilleniaceae) against Candida Species
title_sort antifungal properties of crude extracts, fractions, and purified compounds from bark of curatella americana l. (dilleniaceae) against candida species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548135/
https://www.ncbi.nlm.nih.gov/pubmed/26347790
http://dx.doi.org/10.1155/2015/673962
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