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SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis
The SP100 family members comprise a set of closely related genes on chromosome 2q37.1. The widely expressed SP100 and the leukocyte-specific proteins SP110 and SP140 have been associated with transcriptional regulation and various human diseases. Here, we have characterized the SP100 family member S...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548144/ https://www.ncbi.nlm.nih.gov/pubmed/26347895 http://dx.doi.org/10.1155/2015/526518 |
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author | Saare, Mario Hämarik, Uku Venta, Rainis Panarina, Marina Zucchelli, Chiara Pihlap, Maire Remm, Anu Kisand, Kai Toots, Urve Möll, Kaidi Salupere, Riina Musco, Giovanna Uibo, Raivo Peterson, Pärt |
author_facet | Saare, Mario Hämarik, Uku Venta, Rainis Panarina, Marina Zucchelli, Chiara Pihlap, Maire Remm, Anu Kisand, Kai Toots, Urve Möll, Kaidi Salupere, Riina Musco, Giovanna Uibo, Raivo Peterson, Pärt |
author_sort | Saare, Mario |
collection | PubMed |
description | The SP100 family members comprise a set of closely related genes on chromosome 2q37.1. The widely expressed SP100 and the leukocyte-specific proteins SP110 and SP140 have been associated with transcriptional regulation and various human diseases. Here, we have characterized the SP100 family member SP140L. The genome sequence analysis showed the formation of SP140L gene through rearrangements of the two neighboring genes, SP100 and SP140, during the evolution of higher primates. The SP140L expression is interferon-inducible with high transcript levels in B cells and other peripheral blood mononuclear cells. Subcellularly, SP140L colocalizes with SP100 and SP140 in nuclear structures that are devoid of SP110, PML, or p300 proteins. Similarly to SP100 and SP140 protein, we detected serum autoantibodies to SP140L in patients with primary biliary cirrhosis using luciferase immunoprecipitation system and immunoblotting assays. In conclusion, our results show that SP140L is phylogenetically recent member of SP100 proteins and acts as an autoantigen in primary biliary cirrhosis patients. |
format | Online Article Text |
id | pubmed-4548144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-45481442015-09-07 SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis Saare, Mario Hämarik, Uku Venta, Rainis Panarina, Marina Zucchelli, Chiara Pihlap, Maire Remm, Anu Kisand, Kai Toots, Urve Möll, Kaidi Salupere, Riina Musco, Giovanna Uibo, Raivo Peterson, Pärt J Immunol Res Research Article The SP100 family members comprise a set of closely related genes on chromosome 2q37.1. The widely expressed SP100 and the leukocyte-specific proteins SP110 and SP140 have been associated with transcriptional regulation and various human diseases. Here, we have characterized the SP100 family member SP140L. The genome sequence analysis showed the formation of SP140L gene through rearrangements of the two neighboring genes, SP100 and SP140, during the evolution of higher primates. The SP140L expression is interferon-inducible with high transcript levels in B cells and other peripheral blood mononuclear cells. Subcellularly, SP140L colocalizes with SP100 and SP140 in nuclear structures that are devoid of SP110, PML, or p300 proteins. Similarly to SP100 and SP140 protein, we detected serum autoantibodies to SP140L in patients with primary biliary cirrhosis using luciferase immunoprecipitation system and immunoblotting assays. In conclusion, our results show that SP140L is phylogenetically recent member of SP100 proteins and acts as an autoantigen in primary biliary cirrhosis patients. Hindawi Publishing Corporation 2015 2015-08-11 /pmc/articles/PMC4548144/ /pubmed/26347895 http://dx.doi.org/10.1155/2015/526518 Text en Copyright © 2015 Mario Saare et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Saare, Mario Hämarik, Uku Venta, Rainis Panarina, Marina Zucchelli, Chiara Pihlap, Maire Remm, Anu Kisand, Kai Toots, Urve Möll, Kaidi Salupere, Riina Musco, Giovanna Uibo, Raivo Peterson, Pärt SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis |
title | SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis |
title_full | SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis |
title_fullStr | SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis |
title_full_unstemmed | SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis |
title_short | SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis |
title_sort | sp140l, an evolutionarily recent member of the sp100 family, is an autoantigen in primary biliary cirrhosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548144/ https://www.ncbi.nlm.nih.gov/pubmed/26347895 http://dx.doi.org/10.1155/2015/526518 |
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