Cargando…

Phenotypic and Molecular Epidemiology of Acinetobacter calcoaceticus baumannii Complex Strains Spread at Nemazee Hospital of Shiraz, Iran

BACKGROUND: Acinetobacter calcoaceticus baumannii (ACB) complex are Gram-negative opportunistic bacteria with low virulence properties. Their resistance to antibiotics has become a matter of concern in hospital infections. OBJECTIVES: The present study aimed to determine the prevalence and antimicro...

Descripción completa

Detalles Bibliográficos
Autores principales: Anvarinejad, Mojtaba, Japoni, Aziz, Davarpanah, Mohammad Ali, Mahmudi, Hossein, Mammina, Caterina, Vazin, Afsaneh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548468/
https://www.ncbi.nlm.nih.gov/pubmed/26322204
http://dx.doi.org/10.5812/jjm.8(5)2015.19180
_version_ 1782387197457989632
author Anvarinejad, Mojtaba
Japoni, Aziz
Davarpanah, Mohammad Ali
Mahmudi, Hossein
Mammina, Caterina
Vazin, Afsaneh
author_facet Anvarinejad, Mojtaba
Japoni, Aziz
Davarpanah, Mohammad Ali
Mahmudi, Hossein
Mammina, Caterina
Vazin, Afsaneh
author_sort Anvarinejad, Mojtaba
collection PubMed
description BACKGROUND: Acinetobacter calcoaceticus baumannii (ACB) complex are Gram-negative opportunistic bacteria with low virulence properties. Their resistance to antibiotics has become a matter of concern in hospital infections. OBJECTIVES: The present study aimed to determine the prevalence and antimicrobial susceptibility of ACB isolates collected from the Nemazee hospital of Shiraz. In addition, Pulsed Field Gel Electrophoresis (PFGE) was used to determine the genetic patterns of these strains. PATIENTS AND METHODS: In this cross-sectional study, 93 strains of ACB complex were isolated from patients of Nemazee hospital, Shiraz, Iran. The antibiotic susceptibility patterns of the isolates to the following 15 antibiotics were determined: gentamicin, ticarcillin, ceftazidime, co-trimoxazole, imipenem, piperacillin tazobactam, amikacin, aztreonam, sulbactam, meropenem, tobramycin, cefotaxime, ceftriaxone, colistin, polymyxin B. Pulsed Field Gel Electrophoresis was used to determine the clonal relationship of these strains. RESULTS: Most of the isolates were found to be resistant to cefotaxime, co-trimoxazole, ceftriaxone, aztreonam, ceftazidime and ticarcillin (90%), and the least resistance was observed to colistin and polymyxin B. Among the 93 tested samples, 35 antimicrobial susceptibility patterns and 47 PFGE patterns were obtained. CONCLUSIONS: High resistance to antibiotics was observed among the strains of ACB complex and the least resistance was towards colistin and polymyxin B, indicating that these antibiotics could be effective for treatment, in case there is no other choice. Using PFGE, the similarity between some strains of Acinetobacter was determined, which indicated epidemics in different parts of the hospital; such epidemics can in turn lead to increased incidence of Acinetobacter infections.
format Online
Article
Text
id pubmed-4548468
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Kowsar
record_format MEDLINE/PubMed
spelling pubmed-45484682015-08-28 Phenotypic and Molecular Epidemiology of Acinetobacter calcoaceticus baumannii Complex Strains Spread at Nemazee Hospital of Shiraz, Iran Anvarinejad, Mojtaba Japoni, Aziz Davarpanah, Mohammad Ali Mahmudi, Hossein Mammina, Caterina Vazin, Afsaneh Jundishapur J Microbiol Research Article BACKGROUND: Acinetobacter calcoaceticus baumannii (ACB) complex are Gram-negative opportunistic bacteria with low virulence properties. Their resistance to antibiotics has become a matter of concern in hospital infections. OBJECTIVES: The present study aimed to determine the prevalence and antimicrobial susceptibility of ACB isolates collected from the Nemazee hospital of Shiraz. In addition, Pulsed Field Gel Electrophoresis (PFGE) was used to determine the genetic patterns of these strains. PATIENTS AND METHODS: In this cross-sectional study, 93 strains of ACB complex were isolated from patients of Nemazee hospital, Shiraz, Iran. The antibiotic susceptibility patterns of the isolates to the following 15 antibiotics were determined: gentamicin, ticarcillin, ceftazidime, co-trimoxazole, imipenem, piperacillin tazobactam, amikacin, aztreonam, sulbactam, meropenem, tobramycin, cefotaxime, ceftriaxone, colistin, polymyxin B. Pulsed Field Gel Electrophoresis was used to determine the clonal relationship of these strains. RESULTS: Most of the isolates were found to be resistant to cefotaxime, co-trimoxazole, ceftriaxone, aztreonam, ceftazidime and ticarcillin (90%), and the least resistance was observed to colistin and polymyxin B. Among the 93 tested samples, 35 antimicrobial susceptibility patterns and 47 PFGE patterns were obtained. CONCLUSIONS: High resistance to antibiotics was observed among the strains of ACB complex and the least resistance was towards colistin and polymyxin B, indicating that these antibiotics could be effective for treatment, in case there is no other choice. Using PFGE, the similarity between some strains of Acinetobacter was determined, which indicated epidemics in different parts of the hospital; such epidemics can in turn lead to increased incidence of Acinetobacter infections. Kowsar 2015-06-20 /pmc/articles/PMC4548468/ /pubmed/26322204 http://dx.doi.org/10.5812/jjm.8(5)2015.19180 Text en Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Anvarinejad, Mojtaba
Japoni, Aziz
Davarpanah, Mohammad Ali
Mahmudi, Hossein
Mammina, Caterina
Vazin, Afsaneh
Phenotypic and Molecular Epidemiology of Acinetobacter calcoaceticus baumannii Complex Strains Spread at Nemazee Hospital of Shiraz, Iran
title Phenotypic and Molecular Epidemiology of Acinetobacter calcoaceticus baumannii Complex Strains Spread at Nemazee Hospital of Shiraz, Iran
title_full Phenotypic and Molecular Epidemiology of Acinetobacter calcoaceticus baumannii Complex Strains Spread at Nemazee Hospital of Shiraz, Iran
title_fullStr Phenotypic and Molecular Epidemiology of Acinetobacter calcoaceticus baumannii Complex Strains Spread at Nemazee Hospital of Shiraz, Iran
title_full_unstemmed Phenotypic and Molecular Epidemiology of Acinetobacter calcoaceticus baumannii Complex Strains Spread at Nemazee Hospital of Shiraz, Iran
title_short Phenotypic and Molecular Epidemiology of Acinetobacter calcoaceticus baumannii Complex Strains Spread at Nemazee Hospital of Shiraz, Iran
title_sort phenotypic and molecular epidemiology of acinetobacter calcoaceticus baumannii complex strains spread at nemazee hospital of shiraz, iran
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548468/
https://www.ncbi.nlm.nih.gov/pubmed/26322204
http://dx.doi.org/10.5812/jjm.8(5)2015.19180
work_keys_str_mv AT anvarinejadmojtaba phenotypicandmolecularepidemiologyofacinetobactercalcoaceticusbaumanniicomplexstrainsspreadatnemazeehospitalofshiraziran
AT japoniaziz phenotypicandmolecularepidemiologyofacinetobactercalcoaceticusbaumanniicomplexstrainsspreadatnemazeehospitalofshiraziran
AT davarpanahmohammadali phenotypicandmolecularepidemiologyofacinetobactercalcoaceticusbaumanniicomplexstrainsspreadatnemazeehospitalofshiraziran
AT mahmudihossein phenotypicandmolecularepidemiologyofacinetobactercalcoaceticusbaumanniicomplexstrainsspreadatnemazeehospitalofshiraziran
AT mamminacaterina phenotypicandmolecularepidemiologyofacinetobactercalcoaceticusbaumanniicomplexstrainsspreadatnemazeehospitalofshiraziran
AT vazinafsaneh phenotypicandmolecularepidemiologyofacinetobactercalcoaceticusbaumanniicomplexstrainsspreadatnemazeehospitalofshiraziran