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Chagas disease reactivation in a heart transplant patient infected by domestic Trypanosoma cruzi discrete typing unit I (TcI(DOM))

BACKGROUND: Trypanosoma cruzi, causative agent of Chagas disease, displays high intraspecific genetic diversity: six genetic lineages or discrete typing units (DTUs) are currently recognized, termed TcI through TcVI. Each DTU presents a particular distribution pattern across the Americas, and is loo...

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Autores principales: Costales, Jaime A., Kotton, Camille N., Zurita-Leal, Andrea C., Garcia-Perez, Josselyn, Llewellyn, Martin S., Messenger, Louisa A., Bhattacharyya, Tapan, Burleigh, Barbara A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548706/
https://www.ncbi.nlm.nih.gov/pubmed/26303927
http://dx.doi.org/10.1186/s13071-015-1039-3
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author Costales, Jaime A.
Kotton, Camille N.
Zurita-Leal, Andrea C.
Garcia-Perez, Josselyn
Llewellyn, Martin S.
Messenger, Louisa A.
Bhattacharyya, Tapan
Burleigh, Barbara A.
author_facet Costales, Jaime A.
Kotton, Camille N.
Zurita-Leal, Andrea C.
Garcia-Perez, Josselyn
Llewellyn, Martin S.
Messenger, Louisa A.
Bhattacharyya, Tapan
Burleigh, Barbara A.
author_sort Costales, Jaime A.
collection PubMed
description BACKGROUND: Trypanosoma cruzi, causative agent of Chagas disease, displays high intraspecific genetic diversity: six genetic lineages or discrete typing units (DTUs) are currently recognized, termed TcI through TcVI. Each DTU presents a particular distribution pattern across the Americas, and is loosely associated with different transmission cycles and hosts. Several DTUs are known to circulate in Central America. It has been previously suggested that TcI infection is benign and does not lead to chronic chagasic cardiomyopathy (CCC). FINDINGS: In this study, we genotyped T. cruzi parasites circulating in the blood and from explanted cardiac tissue of an El Salvadorian patient who developed reactivation Chagas disease while on immunosuppressive medications after undergoing heart transplant in the U.S. as treatment for end-stage CCC. Parasite typing was performed through molecular methods (restriction fragment length polymorphism of polymerase reaction chain amplified products, microsatellite typing, maxicircle sequence typing and low-stringency single primer PCR, [LSSP-PCR]) as well as lineage-specific serology. We show that the parasites infecting the patient belong to the TcI DTU exclusively. Our data indicate that the parasites isolated from the patient belong to a genotype frequently associated with human infection throughout the Americas (TcI(DOM)). CONCLUSIONS: Our results constitute compelling evidence in support of TcI DTU’s ability to cause end-stage CCC and help dispel any residual bias that infection with this lineage is benign, pointing to the need for increased surveillance for dissemination of this genotype in endemic regions, the USA and globally.
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spelling pubmed-45487062015-08-26 Chagas disease reactivation in a heart transplant patient infected by domestic Trypanosoma cruzi discrete typing unit I (TcI(DOM)) Costales, Jaime A. Kotton, Camille N. Zurita-Leal, Andrea C. Garcia-Perez, Josselyn Llewellyn, Martin S. Messenger, Louisa A. Bhattacharyya, Tapan Burleigh, Barbara A. Parasit Vectors Short Report BACKGROUND: Trypanosoma cruzi, causative agent of Chagas disease, displays high intraspecific genetic diversity: six genetic lineages or discrete typing units (DTUs) are currently recognized, termed TcI through TcVI. Each DTU presents a particular distribution pattern across the Americas, and is loosely associated with different transmission cycles and hosts. Several DTUs are known to circulate in Central America. It has been previously suggested that TcI infection is benign and does not lead to chronic chagasic cardiomyopathy (CCC). FINDINGS: In this study, we genotyped T. cruzi parasites circulating in the blood and from explanted cardiac tissue of an El Salvadorian patient who developed reactivation Chagas disease while on immunosuppressive medications after undergoing heart transplant in the U.S. as treatment for end-stage CCC. Parasite typing was performed through molecular methods (restriction fragment length polymorphism of polymerase reaction chain amplified products, microsatellite typing, maxicircle sequence typing and low-stringency single primer PCR, [LSSP-PCR]) as well as lineage-specific serology. We show that the parasites infecting the patient belong to the TcI DTU exclusively. Our data indicate that the parasites isolated from the patient belong to a genotype frequently associated with human infection throughout the Americas (TcI(DOM)). CONCLUSIONS: Our results constitute compelling evidence in support of TcI DTU’s ability to cause end-stage CCC and help dispel any residual bias that infection with this lineage is benign, pointing to the need for increased surveillance for dissemination of this genotype in endemic regions, the USA and globally. BioMed Central 2015-08-25 /pmc/articles/PMC4548706/ /pubmed/26303927 http://dx.doi.org/10.1186/s13071-015-1039-3 Text en © Costales et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Costales, Jaime A.
Kotton, Camille N.
Zurita-Leal, Andrea C.
Garcia-Perez, Josselyn
Llewellyn, Martin S.
Messenger, Louisa A.
Bhattacharyya, Tapan
Burleigh, Barbara A.
Chagas disease reactivation in a heart transplant patient infected by domestic Trypanosoma cruzi discrete typing unit I (TcI(DOM))
title Chagas disease reactivation in a heart transplant patient infected by domestic Trypanosoma cruzi discrete typing unit I (TcI(DOM))
title_full Chagas disease reactivation in a heart transplant patient infected by domestic Trypanosoma cruzi discrete typing unit I (TcI(DOM))
title_fullStr Chagas disease reactivation in a heart transplant patient infected by domestic Trypanosoma cruzi discrete typing unit I (TcI(DOM))
title_full_unstemmed Chagas disease reactivation in a heart transplant patient infected by domestic Trypanosoma cruzi discrete typing unit I (TcI(DOM))
title_short Chagas disease reactivation in a heart transplant patient infected by domestic Trypanosoma cruzi discrete typing unit I (TcI(DOM))
title_sort chagas disease reactivation in a heart transplant patient infected by domestic trypanosoma cruzi discrete typing unit i (tci(dom))
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548706/
https://www.ncbi.nlm.nih.gov/pubmed/26303927
http://dx.doi.org/10.1186/s13071-015-1039-3
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