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The molecular fingerprint of lung inflammation after blunt chest trauma

BACKGROUND: After severe blunt chest trauma, the development of an acute lung injury (ALI) is often associated with severe or even lethal complications. Especially in multiple injured patients after blunt chest trauma ALI/ARDS [acute respiratory distress syndrome (ARDS)] is frequent. However, in the...

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Autores principales: Ehrnthaller, Christian, Flierl, Michael, Perl, Mario, Denk, Stephanie, Unnewehr, Heike, Ward, Peter A., Radermacher, Peter, Ignatius, Anita, Gebhard, Florian, Chinnaiyan, Arul, Huber-Lang, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548898/
https://www.ncbi.nlm.nih.gov/pubmed/26303896
http://dx.doi.org/10.1186/s40001-015-0164-y
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author Ehrnthaller, Christian
Flierl, Michael
Perl, Mario
Denk, Stephanie
Unnewehr, Heike
Ward, Peter A.
Radermacher, Peter
Ignatius, Anita
Gebhard, Florian
Chinnaiyan, Arul
Huber-Lang, Markus
author_facet Ehrnthaller, Christian
Flierl, Michael
Perl, Mario
Denk, Stephanie
Unnewehr, Heike
Ward, Peter A.
Radermacher, Peter
Ignatius, Anita
Gebhard, Florian
Chinnaiyan, Arul
Huber-Lang, Markus
author_sort Ehrnthaller, Christian
collection PubMed
description BACKGROUND: After severe blunt chest trauma, the development of an acute lung injury (ALI) is often associated with severe or even lethal complications. Especially in multiple injured patients after blunt chest trauma ALI/ARDS [acute respiratory distress syndrome (ARDS)] is frequent. However, in the initial posttraumatic phase, inflammatory clinical signs are often not apparent and underlying changes in gene-expression profile are unknown. METHODS: Therefore, inflammation in lung tissue following blunt chest trauma was characterized in a well-defined bilateral lung injury model. Using DNA microarrays representing 9240 genes, the temporal sequence of blunt chest trauma-induced gene-expression patterns in lung tissue was examined. RESULTS: The results suggest an activation of a highly complex transcriptional program in response to chest trauma. Chest trauma led to elevated expression levels of inflammatory and coagulatory proteins (such as TNFα receptor, IL-1α, IL-1β, C3, NF-κB and plasminogen activator). However, upregulation of proteins was found, usually incoherent of exerting effects in blunt thoracic trauma (pendrin, resistin, metallothionein and glucocorticoid-induced leucine zipper). Furthermore, significant downregulation was observed as early as 10 min after trauma for cytokines and complement factors (LCR-1, C4) as well as for intracellular signaling molecules (inhibitory protein phosphatase) and ion-channels (voltage-dependent Ca(2+) channel). CONCLUSIONS: Taken together, the provided global perspective of the inflammatory response following blunt chest trauma could provide a molecular framework for future research in trauma pathophysiology.
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spelling pubmed-45488982015-08-26 The molecular fingerprint of lung inflammation after blunt chest trauma Ehrnthaller, Christian Flierl, Michael Perl, Mario Denk, Stephanie Unnewehr, Heike Ward, Peter A. Radermacher, Peter Ignatius, Anita Gebhard, Florian Chinnaiyan, Arul Huber-Lang, Markus Eur J Med Res Research BACKGROUND: After severe blunt chest trauma, the development of an acute lung injury (ALI) is often associated with severe or even lethal complications. Especially in multiple injured patients after blunt chest trauma ALI/ARDS [acute respiratory distress syndrome (ARDS)] is frequent. However, in the initial posttraumatic phase, inflammatory clinical signs are often not apparent and underlying changes in gene-expression profile are unknown. METHODS: Therefore, inflammation in lung tissue following blunt chest trauma was characterized in a well-defined bilateral lung injury model. Using DNA microarrays representing 9240 genes, the temporal sequence of blunt chest trauma-induced gene-expression patterns in lung tissue was examined. RESULTS: The results suggest an activation of a highly complex transcriptional program in response to chest trauma. Chest trauma led to elevated expression levels of inflammatory and coagulatory proteins (such as TNFα receptor, IL-1α, IL-1β, C3, NF-κB and plasminogen activator). However, upregulation of proteins was found, usually incoherent of exerting effects in blunt thoracic trauma (pendrin, resistin, metallothionein and glucocorticoid-induced leucine zipper). Furthermore, significant downregulation was observed as early as 10 min after trauma for cytokines and complement factors (LCR-1, C4) as well as for intracellular signaling molecules (inhibitory protein phosphatase) and ion-channels (voltage-dependent Ca(2+) channel). CONCLUSIONS: Taken together, the provided global perspective of the inflammatory response following blunt chest trauma could provide a molecular framework for future research in trauma pathophysiology. BioMed Central 2015-08-25 /pmc/articles/PMC4548898/ /pubmed/26303896 http://dx.doi.org/10.1186/s40001-015-0164-y Text en © Ehrnthaller et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ehrnthaller, Christian
Flierl, Michael
Perl, Mario
Denk, Stephanie
Unnewehr, Heike
Ward, Peter A.
Radermacher, Peter
Ignatius, Anita
Gebhard, Florian
Chinnaiyan, Arul
Huber-Lang, Markus
The molecular fingerprint of lung inflammation after blunt chest trauma
title The molecular fingerprint of lung inflammation after blunt chest trauma
title_full The molecular fingerprint of lung inflammation after blunt chest trauma
title_fullStr The molecular fingerprint of lung inflammation after blunt chest trauma
title_full_unstemmed The molecular fingerprint of lung inflammation after blunt chest trauma
title_short The molecular fingerprint of lung inflammation after blunt chest trauma
title_sort molecular fingerprint of lung inflammation after blunt chest trauma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548898/
https://www.ncbi.nlm.nih.gov/pubmed/26303896
http://dx.doi.org/10.1186/s40001-015-0164-y
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