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Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT(™)) and standard density gradient
BACKGROUND: High quality genetic material is an essential pre-requisite when analyzing gene expression using microarray technology. Peripheral blood mononuclear cells (PBMC) are frequently used for genomic analyses, but several factors can affect the integrity of nucleic acids prior to their extract...
Autores principales: | Corkum, Christopher P., Ings, Danielle P., Burgess, Christopher, Karwowska, Sylwia, Kroll, Werner, Michalak, Tomasz I. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4549105/ https://www.ncbi.nlm.nih.gov/pubmed/26307036 http://dx.doi.org/10.1186/s12865-015-0113-0 |
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