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Systems Genetics Reveals the Functional Context of PCOS Loci and Identifies Genetic and Molecular Mechanisms of Disease Heterogeneity

Genome wide association studies (GWAS) have revealed 11 independent risk loci for polycystic ovary syndrome (PCOS), a common disorder in young women characterized by androgen excess and oligomenorrhea. To put these risk loci and the single nucleotide polymorphisms (SNPs) therein into functional cont...

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Autores principales: Jones, Michelle R., Brower, Meredith A., Xu, Ning, Cui, Jinrui, Mengesha, Emebet, Chen, Yii-Der I., Taylor, Kent D., Azziz, Ricardo, Goodarzi, Mark O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4549292/
https://www.ncbi.nlm.nih.gov/pubmed/26305227
http://dx.doi.org/10.1371/journal.pgen.1005455
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author Jones, Michelle R.
Brower, Meredith A.
Xu, Ning
Cui, Jinrui
Mengesha, Emebet
Chen, Yii-Der I.
Taylor, Kent D.
Azziz, Ricardo
Goodarzi, Mark O.
author_facet Jones, Michelle R.
Brower, Meredith A.
Xu, Ning
Cui, Jinrui
Mengesha, Emebet
Chen, Yii-Der I.
Taylor, Kent D.
Azziz, Ricardo
Goodarzi, Mark O.
author_sort Jones, Michelle R.
collection PubMed
description Genome wide association studies (GWAS) have revealed 11 independent risk loci for polycystic ovary syndrome (PCOS), a common disorder in young women characterized by androgen excess and oligomenorrhea. To put these risk loci and the single nucleotide polymorphisms (SNPs) therein into functional context, we measured DNA methylation and gene expression in subcutaneous adipose tissue biopsies to identify PCOS-specific alterations. Two genes from the LHCGR region, STON1-GTF2A1L and LHCGR, were overexpressed in PCOS. In analysis stratified by obesity, LHCGR was overexpressed only in non-obese PCOS women. Although not differentially expressed in the entire PCOS group, INSR was underexpressed in obese PCOS subjects only. Alterations in gene expression in the LHCGR, RAB5B and INSR regions suggest that SNPs in these loci may be functional and could affect gene expression directly or indirectly via epigenetic alterations. We identified reduced methylation in the LHCGR locus and increased methylation in the INSR locus, changes that are concordant with the altered gene expression profiles. Complex patterns of meQTL and eQTL were identified in these loci, suggesting that local genetic variation plays an important role in gene regulation. We propose that non-obese PCOS women possess significant alterations in LH receptor expression, which drives excess androgen secretion from the ovary. Alternatively, obese women with PCOS possess alterations in insulin receptor expression, with underexpression in metabolic tissues and overexpression in the ovary, resulting in peripheral insulin resistance and excess ovarian androgen production. These studies provide a genetic and molecular basis for the reported clinical heterogeneity of PCOS.
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spelling pubmed-45492922015-09-01 Systems Genetics Reveals the Functional Context of PCOS Loci and Identifies Genetic and Molecular Mechanisms of Disease Heterogeneity Jones, Michelle R. Brower, Meredith A. Xu, Ning Cui, Jinrui Mengesha, Emebet Chen, Yii-Der I. Taylor, Kent D. Azziz, Ricardo Goodarzi, Mark O. PLoS Genet Research Article Genome wide association studies (GWAS) have revealed 11 independent risk loci for polycystic ovary syndrome (PCOS), a common disorder in young women characterized by androgen excess and oligomenorrhea. To put these risk loci and the single nucleotide polymorphisms (SNPs) therein into functional context, we measured DNA methylation and gene expression in subcutaneous adipose tissue biopsies to identify PCOS-specific alterations. Two genes from the LHCGR region, STON1-GTF2A1L and LHCGR, were overexpressed in PCOS. In analysis stratified by obesity, LHCGR was overexpressed only in non-obese PCOS women. Although not differentially expressed in the entire PCOS group, INSR was underexpressed in obese PCOS subjects only. Alterations in gene expression in the LHCGR, RAB5B and INSR regions suggest that SNPs in these loci may be functional and could affect gene expression directly or indirectly via epigenetic alterations. We identified reduced methylation in the LHCGR locus and increased methylation in the INSR locus, changes that are concordant with the altered gene expression profiles. Complex patterns of meQTL and eQTL were identified in these loci, suggesting that local genetic variation plays an important role in gene regulation. We propose that non-obese PCOS women possess significant alterations in LH receptor expression, which drives excess androgen secretion from the ovary. Alternatively, obese women with PCOS possess alterations in insulin receptor expression, with underexpression in metabolic tissues and overexpression in the ovary, resulting in peripheral insulin resistance and excess ovarian androgen production. These studies provide a genetic and molecular basis for the reported clinical heterogeneity of PCOS. Public Library of Science 2015-08-25 /pmc/articles/PMC4549292/ /pubmed/26305227 http://dx.doi.org/10.1371/journal.pgen.1005455 Text en © 2015 Jones et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jones, Michelle R.
Brower, Meredith A.
Xu, Ning
Cui, Jinrui
Mengesha, Emebet
Chen, Yii-Der I.
Taylor, Kent D.
Azziz, Ricardo
Goodarzi, Mark O.
Systems Genetics Reveals the Functional Context of PCOS Loci and Identifies Genetic and Molecular Mechanisms of Disease Heterogeneity
title Systems Genetics Reveals the Functional Context of PCOS Loci and Identifies Genetic and Molecular Mechanisms of Disease Heterogeneity
title_full Systems Genetics Reveals the Functional Context of PCOS Loci and Identifies Genetic and Molecular Mechanisms of Disease Heterogeneity
title_fullStr Systems Genetics Reveals the Functional Context of PCOS Loci and Identifies Genetic and Molecular Mechanisms of Disease Heterogeneity
title_full_unstemmed Systems Genetics Reveals the Functional Context of PCOS Loci and Identifies Genetic and Molecular Mechanisms of Disease Heterogeneity
title_short Systems Genetics Reveals the Functional Context of PCOS Loci and Identifies Genetic and Molecular Mechanisms of Disease Heterogeneity
title_sort systems genetics reveals the functional context of pcos loci and identifies genetic and molecular mechanisms of disease heterogeneity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4549292/
https://www.ncbi.nlm.nih.gov/pubmed/26305227
http://dx.doi.org/10.1371/journal.pgen.1005455
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