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Microbial community profiles of the colon from steers differing in feed efficiency
Ruminal microbial fermentation plays an essential role in host nutrition, and as a result, the rumen microbiota have been a major focus of research examining bovine feed efficiency. Microbial communities within other sections of the gastrointestinal tract may also be important with regard to feed ef...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4549364/ https://www.ncbi.nlm.nih.gov/pubmed/26322260 http://dx.doi.org/10.1186/s40064-015-1201-6 |
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author | Myer, Phillip R Wells, James E Smith, Timothy P L Kuehn, Larry A Freetly, Harvey C |
author_facet | Myer, Phillip R Wells, James E Smith, Timothy P L Kuehn, Larry A Freetly, Harvey C |
author_sort | Myer, Phillip R |
collection | PubMed |
description | Ruminal microbial fermentation plays an essential role in host nutrition, and as a result, the rumen microbiota have been a major focus of research examining bovine feed efficiency. Microbial communities within other sections of the gastrointestinal tract may also be important with regard to feed efficiency, since it is critical to the health and nutrition of the host. The objective of this study was to characterize the microbial communities of the colon among steers differing in feed efficiency. Individual feed intake (FI) and body weight (BW) gain were determined from animals fed the same ration, within two contemporary groups of steers. Four steers from each contemporary group within each Cartesian quadrant were sampled (n = 16/group) from the bivariate distribution of average daily BW gain and average daily FI. Bacterial 16S rRNA gene amplicons were sequenced from the colon content using next-generation sequencing technology. Within the colon content, UniFrac principal coordinate analyses did not detect any separation of microbial communities, and bacterial diversity or richness did not differ between efficiency groups. Relative abundances of microbial populations and operational taxonomic units did reveal significant differences between efficiency groups. The phylum Firmicutes accounted for up to 70% of the populations within all samples, and families Ruminococcaceae and Clostridiaceae were highly abundant. Significant population shifts in taxa were detected, including the families Ruminococcaceae, Lachnospiraceae, and Sphingomonadaceae, and the genera Butyrivibrio, Pseudobutyrivibrio, Prevotella, Faecalibacterium and Oscillospira. This study suggests the association of the colon microbial communities as a factor influencing feed efficiency at the 16S level. |
format | Online Article Text |
id | pubmed-4549364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-45493642015-08-28 Microbial community profiles of the colon from steers differing in feed efficiency Myer, Phillip R Wells, James E Smith, Timothy P L Kuehn, Larry A Freetly, Harvey C Springerplus Research Ruminal microbial fermentation plays an essential role in host nutrition, and as a result, the rumen microbiota have been a major focus of research examining bovine feed efficiency. Microbial communities within other sections of the gastrointestinal tract may also be important with regard to feed efficiency, since it is critical to the health and nutrition of the host. The objective of this study was to characterize the microbial communities of the colon among steers differing in feed efficiency. Individual feed intake (FI) and body weight (BW) gain were determined from animals fed the same ration, within two contemporary groups of steers. Four steers from each contemporary group within each Cartesian quadrant were sampled (n = 16/group) from the bivariate distribution of average daily BW gain and average daily FI. Bacterial 16S rRNA gene amplicons were sequenced from the colon content using next-generation sequencing technology. Within the colon content, UniFrac principal coordinate analyses did not detect any separation of microbial communities, and bacterial diversity or richness did not differ between efficiency groups. Relative abundances of microbial populations and operational taxonomic units did reveal significant differences between efficiency groups. The phylum Firmicutes accounted for up to 70% of the populations within all samples, and families Ruminococcaceae and Clostridiaceae were highly abundant. Significant population shifts in taxa were detected, including the families Ruminococcaceae, Lachnospiraceae, and Sphingomonadaceae, and the genera Butyrivibrio, Pseudobutyrivibrio, Prevotella, Faecalibacterium and Oscillospira. This study suggests the association of the colon microbial communities as a factor influencing feed efficiency at the 16S level. Springer International Publishing 2015-08-27 /pmc/articles/PMC4549364/ /pubmed/26322260 http://dx.doi.org/10.1186/s40064-015-1201-6 Text en © Myer et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Myer, Phillip R Wells, James E Smith, Timothy P L Kuehn, Larry A Freetly, Harvey C Microbial community profiles of the colon from steers differing in feed efficiency |
title | Microbial community profiles of the colon from steers differing in feed efficiency |
title_full | Microbial community profiles of the colon from steers differing in feed efficiency |
title_fullStr | Microbial community profiles of the colon from steers differing in feed efficiency |
title_full_unstemmed | Microbial community profiles of the colon from steers differing in feed efficiency |
title_short | Microbial community profiles of the colon from steers differing in feed efficiency |
title_sort | microbial community profiles of the colon from steers differing in feed efficiency |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4549364/ https://www.ncbi.nlm.nih.gov/pubmed/26322260 http://dx.doi.org/10.1186/s40064-015-1201-6 |
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