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Transposase interaction with the β sliding clamp: effects on insertion sequence proliferation and transposition rate

Insertion sequences (ISs) are ubiquitous and abundant mobile genetic elements in prokaryotic genomes. ISs often encode only one protein, the transposase, which catalyzes their transposition. Recent studies have shown that transposases of many different IS families interact with the β sliding clamp,...

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Autores principales: Díaz-Maldonado, Héctor, Gómez, Manuel J., Moreno-Paz, Mercedes, San Martín-Úriz, Patxi, Amils, Ricardo, Parro, Víctor, López de Saro, Francisco J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4549789/
https://www.ncbi.nlm.nih.gov/pubmed/26306550
http://dx.doi.org/10.1038/srep13329
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author Díaz-Maldonado, Héctor
Gómez, Manuel J.
Moreno-Paz, Mercedes
San Martín-Úriz, Patxi
Amils, Ricardo
Parro, Víctor
López de Saro, Francisco J.
author_facet Díaz-Maldonado, Héctor
Gómez, Manuel J.
Moreno-Paz, Mercedes
San Martín-Úriz, Patxi
Amils, Ricardo
Parro, Víctor
López de Saro, Francisco J.
author_sort Díaz-Maldonado, Héctor
collection PubMed
description Insertion sequences (ISs) are ubiquitous and abundant mobile genetic elements in prokaryotic genomes. ISs often encode only one protein, the transposase, which catalyzes their transposition. Recent studies have shown that transposases of many different IS families interact with the β sliding clamp, a DNA replication factor of the host. However, it was unclear to what extent this interaction limits or favors the ability of ISs to colonize a chromosome from a phylogenetically-distant organism, or if the strength of this interaction affects the transposition rate. Here we describe the proliferation of a member of the IS1634 family in Acidiphilium over ~600 generations of cultured growth. We demonstrate that the purified transposase binds to the β sliding clamp of Acidiphilium, Leptospirillum and E. coli. Further, we also demonstrate that the Acidiphilium IS1634 transposase binds to the archaeal sliding clamp (PCNA) from Methanosarcina, and that the transposase encoded by Methanosarcina IS1634 binds to Acidiphilium β. Finally, we demonstrate that increasing the strength of the interaction between β and transposase results in a higher transposition rate in vivo. Our results suggest that the interaction could determine the potential of ISs to be mobilized in bacterial populations and also their ability to proliferate within chromosomes.
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spelling pubmed-45497892015-09-04 Transposase interaction with the β sliding clamp: effects on insertion sequence proliferation and transposition rate Díaz-Maldonado, Héctor Gómez, Manuel J. Moreno-Paz, Mercedes San Martín-Úriz, Patxi Amils, Ricardo Parro, Víctor López de Saro, Francisco J. Sci Rep Article Insertion sequences (ISs) are ubiquitous and abundant mobile genetic elements in prokaryotic genomes. ISs often encode only one protein, the transposase, which catalyzes their transposition. Recent studies have shown that transposases of many different IS families interact with the β sliding clamp, a DNA replication factor of the host. However, it was unclear to what extent this interaction limits or favors the ability of ISs to colonize a chromosome from a phylogenetically-distant organism, or if the strength of this interaction affects the transposition rate. Here we describe the proliferation of a member of the IS1634 family in Acidiphilium over ~600 generations of cultured growth. We demonstrate that the purified transposase binds to the β sliding clamp of Acidiphilium, Leptospirillum and E. coli. Further, we also demonstrate that the Acidiphilium IS1634 transposase binds to the archaeal sliding clamp (PCNA) from Methanosarcina, and that the transposase encoded by Methanosarcina IS1634 binds to Acidiphilium β. Finally, we demonstrate that increasing the strength of the interaction between β and transposase results in a higher transposition rate in vivo. Our results suggest that the interaction could determine the potential of ISs to be mobilized in bacterial populations and also their ability to proliferate within chromosomes. Nature Publishing Group 2015-08-26 /pmc/articles/PMC4549789/ /pubmed/26306550 http://dx.doi.org/10.1038/srep13329 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Díaz-Maldonado, Héctor
Gómez, Manuel J.
Moreno-Paz, Mercedes
San Martín-Úriz, Patxi
Amils, Ricardo
Parro, Víctor
López de Saro, Francisco J.
Transposase interaction with the β sliding clamp: effects on insertion sequence proliferation and transposition rate
title Transposase interaction with the β sliding clamp: effects on insertion sequence proliferation and transposition rate
title_full Transposase interaction with the β sliding clamp: effects on insertion sequence proliferation and transposition rate
title_fullStr Transposase interaction with the β sliding clamp: effects on insertion sequence proliferation and transposition rate
title_full_unstemmed Transposase interaction with the β sliding clamp: effects on insertion sequence proliferation and transposition rate
title_short Transposase interaction with the β sliding clamp: effects on insertion sequence proliferation and transposition rate
title_sort transposase interaction with the β sliding clamp: effects on insertion sequence proliferation and transposition rate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4549789/
https://www.ncbi.nlm.nih.gov/pubmed/26306550
http://dx.doi.org/10.1038/srep13329
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