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Spinning-Spot Shadowless TIRF Microscopy

Total internal reflection fluorescence (TIRF) microscopy is a powerful tool for visualizing near-membrane cellular structures and processes, including imaging of local Ca(2+) transients with single-channel resolution. TIRF is most commonly implemented in epi-fluorescence mode, whereby laser excitati...

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Detalles Bibliográficos
Autores principales:	Ellefsen, Kyle L., Dynes, Joseph L., Parker, Ian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550233/ https://www.ncbi.nlm.nih.gov/pubmed/26308212 http://dx.doi.org/10.1371/journal.pone.0136055

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