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A New Imaging Platform for Visualizing Biological Effects of Non-Invasive Radiofrequency Electric-Field Cancer Hyperthermia

Herein, we present a novel imaging platform to study the biological effects of non-invasive radiofrequency (RF) electric field cancer hyperthermia. This system allows for real-time in vivo intravital microscopy (IVM) imaging of radiofrequency-induced biological alterations such as changes in vessel...

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Detalles Bibliográficos
Autores principales: Corr, Stuart J., Shamsudeen, Sabeel, Vergara, Leoncio A., Ho, Jason Chak-Shing, Ware, Matthew J., Keshishian, Vazrik, Yokoi, Kenji, Savage, David J., Meraz, Ismail M., Kaluarachchi, Warna, Cisneros, Brandon T., Raoof, Mustafa, Nguyen, Duy Trac, Zhang, Yingchun, Wilson, Lon J., Summers, Huw, Rees, Paul, Curley, Steven A., Serda, Rita E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550384/
https://www.ncbi.nlm.nih.gov/pubmed/26308617
http://dx.doi.org/10.1371/journal.pone.0136382
Descripción
Sumario:Herein, we present a novel imaging platform to study the biological effects of non-invasive radiofrequency (RF) electric field cancer hyperthermia. This system allows for real-time in vivo intravital microscopy (IVM) imaging of radiofrequency-induced biological alterations such as changes in vessel structure and drug perfusion. Our results indicate that the IVM system is able to handle exposure to high-power electric-fields without inducing significant hardware damage or imaging artifacts. Furthermore, short durations of low-power (< 200 W) radiofrequency exposure increased transport and perfusion of fluorescent tracers into the tumors at temperatures below 41°C. Vessel deformations and blood coagulation were seen for tumor temperatures around 44°C. These results highlight the use of our integrated IVM-RF imaging platform as a powerful new tool to visualize the dynamics and interplay between radiofrequency energy and biological tissues, organs, and tumors.