Cargando…

A New Imaging Platform for Visualizing Biological Effects of Non-Invasive Radiofrequency Electric-Field Cancer Hyperthermia

Herein, we present a novel imaging platform to study the biological effects of non-invasive radiofrequency (RF) electric field cancer hyperthermia. This system allows for real-time in vivo intravital microscopy (IVM) imaging of radiofrequency-induced biological alterations such as changes in vessel...

Descripción completa

Detalles Bibliográficos
Autores principales: Corr, Stuart J., Shamsudeen, Sabeel, Vergara, Leoncio A., Ho, Jason Chak-Shing, Ware, Matthew J., Keshishian, Vazrik, Yokoi, Kenji, Savage, David J., Meraz, Ismail M., Kaluarachchi, Warna, Cisneros, Brandon T., Raoof, Mustafa, Nguyen, Duy Trac, Zhang, Yingchun, Wilson, Lon J., Summers, Huw, Rees, Paul, Curley, Steven A., Serda, Rita E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550384/
https://www.ncbi.nlm.nih.gov/pubmed/26308617
http://dx.doi.org/10.1371/journal.pone.0136382
_version_ 1782387447214112768
author Corr, Stuart J.
Shamsudeen, Sabeel
Vergara, Leoncio A.
Ho, Jason Chak-Shing
Ware, Matthew J.
Keshishian, Vazrik
Yokoi, Kenji
Savage, David J.
Meraz, Ismail M.
Kaluarachchi, Warna
Cisneros, Brandon T.
Raoof, Mustafa
Nguyen, Duy Trac
Zhang, Yingchun
Wilson, Lon J.
Summers, Huw
Rees, Paul
Curley, Steven A.
Serda, Rita E.
author_facet Corr, Stuart J.
Shamsudeen, Sabeel
Vergara, Leoncio A.
Ho, Jason Chak-Shing
Ware, Matthew J.
Keshishian, Vazrik
Yokoi, Kenji
Savage, David J.
Meraz, Ismail M.
Kaluarachchi, Warna
Cisneros, Brandon T.
Raoof, Mustafa
Nguyen, Duy Trac
Zhang, Yingchun
Wilson, Lon J.
Summers, Huw
Rees, Paul
Curley, Steven A.
Serda, Rita E.
author_sort Corr, Stuart J.
collection PubMed
description Herein, we present a novel imaging platform to study the biological effects of non-invasive radiofrequency (RF) electric field cancer hyperthermia. This system allows for real-time in vivo intravital microscopy (IVM) imaging of radiofrequency-induced biological alterations such as changes in vessel structure and drug perfusion. Our results indicate that the IVM system is able to handle exposure to high-power electric-fields without inducing significant hardware damage or imaging artifacts. Furthermore, short durations of low-power (< 200 W) radiofrequency exposure increased transport and perfusion of fluorescent tracers into the tumors at temperatures below 41°C. Vessel deformations and blood coagulation were seen for tumor temperatures around 44°C. These results highlight the use of our integrated IVM-RF imaging platform as a powerful new tool to visualize the dynamics and interplay between radiofrequency energy and biological tissues, organs, and tumors.
format Online
Article
Text
id pubmed-4550384
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-45503842015-09-01 A New Imaging Platform for Visualizing Biological Effects of Non-Invasive Radiofrequency Electric-Field Cancer Hyperthermia Corr, Stuart J. Shamsudeen, Sabeel Vergara, Leoncio A. Ho, Jason Chak-Shing Ware, Matthew J. Keshishian, Vazrik Yokoi, Kenji Savage, David J. Meraz, Ismail M. Kaluarachchi, Warna Cisneros, Brandon T. Raoof, Mustafa Nguyen, Duy Trac Zhang, Yingchun Wilson, Lon J. Summers, Huw Rees, Paul Curley, Steven A. Serda, Rita E. PLoS One Research Article Herein, we present a novel imaging platform to study the biological effects of non-invasive radiofrequency (RF) electric field cancer hyperthermia. This system allows for real-time in vivo intravital microscopy (IVM) imaging of radiofrequency-induced biological alterations such as changes in vessel structure and drug perfusion. Our results indicate that the IVM system is able to handle exposure to high-power electric-fields without inducing significant hardware damage or imaging artifacts. Furthermore, short durations of low-power (< 200 W) radiofrequency exposure increased transport and perfusion of fluorescent tracers into the tumors at temperatures below 41°C. Vessel deformations and blood coagulation were seen for tumor temperatures around 44°C. These results highlight the use of our integrated IVM-RF imaging platform as a powerful new tool to visualize the dynamics and interplay between radiofrequency energy and biological tissues, organs, and tumors. Public Library of Science 2015-08-26 /pmc/articles/PMC4550384/ /pubmed/26308617 http://dx.doi.org/10.1371/journal.pone.0136382 Text en © 2015 Corr et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Corr, Stuart J.
Shamsudeen, Sabeel
Vergara, Leoncio A.
Ho, Jason Chak-Shing
Ware, Matthew J.
Keshishian, Vazrik
Yokoi, Kenji
Savage, David J.
Meraz, Ismail M.
Kaluarachchi, Warna
Cisneros, Brandon T.
Raoof, Mustafa
Nguyen, Duy Trac
Zhang, Yingchun
Wilson, Lon J.
Summers, Huw
Rees, Paul
Curley, Steven A.
Serda, Rita E.
A New Imaging Platform for Visualizing Biological Effects of Non-Invasive Radiofrequency Electric-Field Cancer Hyperthermia
title A New Imaging Platform for Visualizing Biological Effects of Non-Invasive Radiofrequency Electric-Field Cancer Hyperthermia
title_full A New Imaging Platform for Visualizing Biological Effects of Non-Invasive Radiofrequency Electric-Field Cancer Hyperthermia
title_fullStr A New Imaging Platform for Visualizing Biological Effects of Non-Invasive Radiofrequency Electric-Field Cancer Hyperthermia
title_full_unstemmed A New Imaging Platform for Visualizing Biological Effects of Non-Invasive Radiofrequency Electric-Field Cancer Hyperthermia
title_short A New Imaging Platform for Visualizing Biological Effects of Non-Invasive Radiofrequency Electric-Field Cancer Hyperthermia
title_sort new imaging platform for visualizing biological effects of non-invasive radiofrequency electric-field cancer hyperthermia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550384/
https://www.ncbi.nlm.nih.gov/pubmed/26308617
http://dx.doi.org/10.1371/journal.pone.0136382
work_keys_str_mv AT corrstuartj anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT shamsudeensabeel anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT vergaraleoncioa anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT hojasonchakshing anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT warematthewj anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT keshishianvazrik anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT yokoikenji anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT savagedavidj anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT merazismailm anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT kaluarachchiwarna anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT cisnerosbrandont anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT raoofmustafa anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT nguyenduytrac anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT zhangyingchun anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT wilsonlonj anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT summershuw anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT reespaul anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT curleystevena anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT serdaritae anewimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT corrstuartj newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT shamsudeensabeel newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT vergaraleoncioa newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT hojasonchakshing newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT warematthewj newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT keshishianvazrik newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT yokoikenji newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT savagedavidj newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT merazismailm newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT kaluarachchiwarna newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT cisnerosbrandont newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT raoofmustafa newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT nguyenduytrac newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT zhangyingchun newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT wilsonlonj newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT summershuw newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT reespaul newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT curleystevena newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia
AT serdaritae newimagingplatformforvisualizingbiologicaleffectsofnoninvasiveradiofrequencyelectricfieldcancerhyperthermia