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Isolation, cloning, and expression of E. coli BirA gene for biotinylation applications

BACKGROUND: The key enzyme in biotin-(strept) avidin systems, Escherichia coli BirA biotin ligase, is currently obtained by overexpression of the long protein-tagged versions of the gene to prevent its toxic effect in E. coli. Herein we describe a rather simple and efficient system for expression of...

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Detalles Bibliográficos
Autores principales:	Etemadzadeh, Mohammad Hossein, Arashkia, Arash, Roohvand, Farzin, Norouzian, Dariush, Azadmanesh, Kayhan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Medknow Publications & Media Pvt Ltd 2015
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551058/ https://www.ncbi.nlm.nih.gov/pubmed/26380234 http://dx.doi.org/10.4103/2277-9175.161576

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