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Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages

Little is known about the molecular players that regulate changes in the endocytic pathway during immune activation. Here we investigate the role of Rab20 in the endocytic pathway during activation of macrophages. Rab20 is associated with endocytic structures, but the function of this Rab GTPase in...

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Autores principales: Pei, Gang, Schnettger, Laura, Bronietzki, Marc, Repnik, Urska, Griffiths, Gareth, Gutierrez, Maximiliano Gabriel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551319/
https://www.ncbi.nlm.nih.gov/pubmed/26157167
http://dx.doi.org/10.1091/mbc.E14-11-1547
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author Pei, Gang
Schnettger, Laura
Bronietzki, Marc
Repnik, Urska
Griffiths, Gareth
Gutierrez, Maximiliano Gabriel
author_facet Pei, Gang
Schnettger, Laura
Bronietzki, Marc
Repnik, Urska
Griffiths, Gareth
Gutierrez, Maximiliano Gabriel
author_sort Pei, Gang
collection PubMed
description Little is known about the molecular players that regulate changes in the endocytic pathway during immune activation. Here we investigate the role of Rab20 in the endocytic pathway during activation of macrophages. Rab20 is associated with endocytic structures, but the function of this Rab GTPase in the endocytic pathway remains poorly characterized. We find that in macrophages, Rab20 expression and endosomal association significantly increase after interferon-γ (IFN-γ) treatment. Moreover, IFN-γ and Rab20 expression induce a dramatic enlargement of endosomes. These enlarged endosomes are the result of homotypic fusion promoted by Rab20 expression. The expression of Rab20 or the dominant-negative mutant Rab20T19N does not affect transferrin or dextran 70 kDa uptake. However, knockdown of Rab20 accelerates epidermal growth factor (EGF) trafficking to LAMP-2–positive compartments and EGF receptor degradation. Thus this work defines a function for Rab20 in the endocytic pathway during immune activation of macrophages.
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spelling pubmed-45513192015-11-16 Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages Pei, Gang Schnettger, Laura Bronietzki, Marc Repnik, Urska Griffiths, Gareth Gutierrez, Maximiliano Gabriel Mol Biol Cell Articles Little is known about the molecular players that regulate changes in the endocytic pathway during immune activation. Here we investigate the role of Rab20 in the endocytic pathway during activation of macrophages. Rab20 is associated with endocytic structures, but the function of this Rab GTPase in the endocytic pathway remains poorly characterized. We find that in macrophages, Rab20 expression and endosomal association significantly increase after interferon-γ (IFN-γ) treatment. Moreover, IFN-γ and Rab20 expression induce a dramatic enlargement of endosomes. These enlarged endosomes are the result of homotypic fusion promoted by Rab20 expression. The expression of Rab20 or the dominant-negative mutant Rab20T19N does not affect transferrin or dextran 70 kDa uptake. However, knockdown of Rab20 accelerates epidermal growth factor (EGF) trafficking to LAMP-2–positive compartments and EGF receptor degradation. Thus this work defines a function for Rab20 in the endocytic pathway during immune activation of macrophages. The American Society for Cell Biology 2015-09-01 /pmc/articles/PMC4551319/ /pubmed/26157167 http://dx.doi.org/10.1091/mbc.E14-11-1547 Text en © 2015 Pei, Schnettger, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Pei, Gang
Schnettger, Laura
Bronietzki, Marc
Repnik, Urska
Griffiths, Gareth
Gutierrez, Maximiliano Gabriel
Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages
title Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages
title_full Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages
title_fullStr Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages
title_full_unstemmed Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages
title_short Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages
title_sort interferon-γ–inducible rab20 regulates endosomal morphology and egfr degradation in macrophages
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551319/
https://www.ncbi.nlm.nih.gov/pubmed/26157167
http://dx.doi.org/10.1091/mbc.E14-11-1547
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