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Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages
Little is known about the molecular players that regulate changes in the endocytic pathway during immune activation. Here we investigate the role of Rab20 in the endocytic pathway during activation of macrophages. Rab20 is associated with endocytic structures, but the function of this Rab GTPase in...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551319/ https://www.ncbi.nlm.nih.gov/pubmed/26157167 http://dx.doi.org/10.1091/mbc.E14-11-1547 |
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author | Pei, Gang Schnettger, Laura Bronietzki, Marc Repnik, Urska Griffiths, Gareth Gutierrez, Maximiliano Gabriel |
author_facet | Pei, Gang Schnettger, Laura Bronietzki, Marc Repnik, Urska Griffiths, Gareth Gutierrez, Maximiliano Gabriel |
author_sort | Pei, Gang |
collection | PubMed |
description | Little is known about the molecular players that regulate changes in the endocytic pathway during immune activation. Here we investigate the role of Rab20 in the endocytic pathway during activation of macrophages. Rab20 is associated with endocytic structures, but the function of this Rab GTPase in the endocytic pathway remains poorly characterized. We find that in macrophages, Rab20 expression and endosomal association significantly increase after interferon-γ (IFN-γ) treatment. Moreover, IFN-γ and Rab20 expression induce a dramatic enlargement of endosomes. These enlarged endosomes are the result of homotypic fusion promoted by Rab20 expression. The expression of Rab20 or the dominant-negative mutant Rab20T19N does not affect transferrin or dextran 70 kDa uptake. However, knockdown of Rab20 accelerates epidermal growth factor (EGF) trafficking to LAMP-2–positive compartments and EGF receptor degradation. Thus this work defines a function for Rab20 in the endocytic pathway during immune activation of macrophages. |
format | Online Article Text |
id | pubmed-4551319 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-45513192015-11-16 Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages Pei, Gang Schnettger, Laura Bronietzki, Marc Repnik, Urska Griffiths, Gareth Gutierrez, Maximiliano Gabriel Mol Biol Cell Articles Little is known about the molecular players that regulate changes in the endocytic pathway during immune activation. Here we investigate the role of Rab20 in the endocytic pathway during activation of macrophages. Rab20 is associated with endocytic structures, but the function of this Rab GTPase in the endocytic pathway remains poorly characterized. We find that in macrophages, Rab20 expression and endosomal association significantly increase after interferon-γ (IFN-γ) treatment. Moreover, IFN-γ and Rab20 expression induce a dramatic enlargement of endosomes. These enlarged endosomes are the result of homotypic fusion promoted by Rab20 expression. The expression of Rab20 or the dominant-negative mutant Rab20T19N does not affect transferrin or dextran 70 kDa uptake. However, knockdown of Rab20 accelerates epidermal growth factor (EGF) trafficking to LAMP-2–positive compartments and EGF receptor degradation. Thus this work defines a function for Rab20 in the endocytic pathway during immune activation of macrophages. The American Society for Cell Biology 2015-09-01 /pmc/articles/PMC4551319/ /pubmed/26157167 http://dx.doi.org/10.1091/mbc.E14-11-1547 Text en © 2015 Pei, Schnettger, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Pei, Gang Schnettger, Laura Bronietzki, Marc Repnik, Urska Griffiths, Gareth Gutierrez, Maximiliano Gabriel Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages |
title | Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages |
title_full | Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages |
title_fullStr | Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages |
title_full_unstemmed | Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages |
title_short | Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages |
title_sort | interferon-γ–inducible rab20 regulates endosomal morphology and egfr degradation in macrophages |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551319/ https://www.ncbi.nlm.nih.gov/pubmed/26157167 http://dx.doi.org/10.1091/mbc.E14-11-1547 |
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