Continuous volumetric imaging via an optical phase-locked ultrasound lens

In vivo imaging at high spatiotemporal resolution holds the key to the fundamental understanding of complex biological systems. Integrating an optical phase-locked ultrasound lens into a conventional two-photon fluorescence microscope, we achieved microsecond scale axial scanning, which enabled high...

Descripción completa

Detalles Bibliográficos
Autores principales: Kong, Lingjie, Tang, Jianyong, Little, Justin P., Yu, Yang, Lämmermann, Tim, Lin, Charles P., Germain, Ronald N., Cui, Meng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551496/
https://www.ncbi.nlm.nih.gov/pubmed/26167641
http://dx.doi.org/10.1038/nmeth.3476
_version_ 1782387576917721088
author Kong, Lingjie
Tang, Jianyong
Little, Justin P.
Yu, Yang
Lämmermann, Tim
Lin, Charles P.
Germain, Ronald N.
Cui, Meng
author_facet Kong, Lingjie
Tang, Jianyong
Little, Justin P.
Yu, Yang
Lämmermann, Tim
Lin, Charles P.
Germain, Ronald N.
Cui, Meng
author_sort Kong, Lingjie
collection PubMed
description In vivo imaging at high spatiotemporal resolution holds the key to the fundamental understanding of complex biological systems. Integrating an optical phase-locked ultrasound lens into a conventional two-photon fluorescence microscope, we achieved microsecond scale axial scanning, which enabled high-speed volumetric imaging. We applied this system to multicolor volumetric imaging of fast processes, including calcium dynamics in the cerebral cortex of behaving mice, and transient morphology changes and trafficking of immune cells.
format Online
Article
Text
id pubmed-4551496
institution National Center for Biotechnology Information
language English
publishDate 2015
record_format MEDLINE/PubMed
spelling pubmed-45514962016-01-31 Continuous volumetric imaging via an optical phase-locked ultrasound lens Kong, Lingjie Tang, Jianyong Little, Justin P. Yu, Yang Lämmermann, Tim Lin, Charles P. Germain, Ronald N. Cui, Meng Nat Methods Article In vivo imaging at high spatiotemporal resolution holds the key to the fundamental understanding of complex biological systems. Integrating an optical phase-locked ultrasound lens into a conventional two-photon fluorescence microscope, we achieved microsecond scale axial scanning, which enabled high-speed volumetric imaging. We applied this system to multicolor volumetric imaging of fast processes, including calcium dynamics in the cerebral cortex of behaving mice, and transient morphology changes and trafficking of immune cells. 2015-07-13 2015-08 /pmc/articles/PMC4551496/ /pubmed/26167641 http://dx.doi.org/10.1038/nmeth.3476 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kong, Lingjie
Tang, Jianyong
Little, Justin P.
Yu, Yang
Lämmermann, Tim
Lin, Charles P.
Germain, Ronald N.
Cui, Meng
Continuous volumetric imaging via an optical phase-locked ultrasound lens
title Continuous volumetric imaging via an optical phase-locked ultrasound lens
title_full Continuous volumetric imaging via an optical phase-locked ultrasound lens
title_fullStr Continuous volumetric imaging via an optical phase-locked ultrasound lens
title_full_unstemmed Continuous volumetric imaging via an optical phase-locked ultrasound lens
title_short Continuous volumetric imaging via an optical phase-locked ultrasound lens
title_sort continuous volumetric imaging via an optical phase-locked ultrasound lens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551496/
https://www.ncbi.nlm.nih.gov/pubmed/26167641
http://dx.doi.org/10.1038/nmeth.3476
work_keys_str_mv AT konglingjie continuousvolumetricimagingviaanopticalphaselockedultrasoundlens
AT tangjianyong continuousvolumetricimagingviaanopticalphaselockedultrasoundlens
AT littlejustinp continuousvolumetricimagingviaanopticalphaselockedultrasoundlens
AT yuyang continuousvolumetricimagingviaanopticalphaselockedultrasoundlens
AT lammermanntim continuousvolumetricimagingviaanopticalphaselockedultrasoundlens
AT lincharlesp continuousvolumetricimagingviaanopticalphaselockedultrasoundlens
AT germainronaldn continuousvolumetricimagingviaanopticalphaselockedultrasoundlens
AT cuimeng continuousvolumetricimagingviaanopticalphaselockedultrasoundlens

Ejemplares similares