Quantification of circulating steroids in individual zebrafish using stacking to achieve nanomolar detection limits with capillary electrophoresis and UV-visible absorbance detection

Capillary electrophoresis and UV-visible absorbance detection are used with sample stacking to achieve detection limits ranging from 0.2 to 2 ng/mL (0.8 to 6 nM) for steroids. Stacking is accomplished using negatively charged cyclodextrin steroid-carrier molecules at a discrete pH interface between...

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Detalles Bibliográficos
Autores principales: Nyakubaya, Vincent T., Durney, Brandon C., Ellington, Marriah C. G., Kantes, Amber D., Reed, Paige A., Walter, Shaylyn E., Stueckle, Jennifer Ripley, Holland, Lisa A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551537/
https://www.ncbi.nlm.nih.gov/pubmed/26041454
http://dx.doi.org/10.1007/s00216-015-8785-0

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