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Progress and future of in vitro models to study translocation of nanoparticles

The increasing use of nanoparticles in products likely results in increased exposure of both workers and consumers. Because of their small size, there are concerns that nanoparticles unintentionally cross the barriers of the human body. Several in vivo rodent studies show that, dependent on the expo...

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Autores principales: Braakhuis, Hedwig M., Kloet, Samantha K., Kezic, Sanja, Kuper, Frieke, Park, Margriet V. D. Z., Bellmann, Susann, van der Zande, Meike, Le Gac, Séverine, Krystek, Petra, Peters, Ruud J. B., Rietjens, Ivonne M. C. M., Bouwmeester, Hans
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551544/
https://www.ncbi.nlm.nih.gov/pubmed/25975987
http://dx.doi.org/10.1007/s00204-015-1518-5
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author Braakhuis, Hedwig M.
Kloet, Samantha K.
Kezic, Sanja
Kuper, Frieke
Park, Margriet V. D. Z.
Bellmann, Susann
van der Zande, Meike
Le Gac, Séverine
Krystek, Petra
Peters, Ruud J. B.
Rietjens, Ivonne M. C. M.
Bouwmeester, Hans
author_facet Braakhuis, Hedwig M.
Kloet, Samantha K.
Kezic, Sanja
Kuper, Frieke
Park, Margriet V. D. Z.
Bellmann, Susann
van der Zande, Meike
Le Gac, Séverine
Krystek, Petra
Peters, Ruud J. B.
Rietjens, Ivonne M. C. M.
Bouwmeester, Hans
author_sort Braakhuis, Hedwig M.
collection PubMed
description The increasing use of nanoparticles in products likely results in increased exposure of both workers and consumers. Because of their small size, there are concerns that nanoparticles unintentionally cross the barriers of the human body. Several in vivo rodent studies show that, dependent on the exposure route, time, and concentration, and their characteristics, nanoparticles can cross the lung, gut, skin, and placental barrier. This review aims to evaluate the performance of in vitro models that mimic the barriers of the human body, with a focus on the lung, gut, skin, and placental barrier. For these barriers, in vitro models of varying complexity are available, ranging from single-cell-type monolayer to multi-cell (3D) models. Only a few studies are available that allow comparison of the in vitro translocation to in vivo data. This situation could change since the availability of analytical detection techniques is no longer a limiting factor for this comparison. We conclude that to further develop in vitro models to be used in risk assessment, the current strategy to improve the models to more closely mimic the human situation by using co-cultures of different cell types and microfluidic approaches to better control the tissue microenvironments are essential. At the current state of the art, the in vitro models do not yet allow prediction of absolute transfer rates but they do support the definition of relative transfer rates and can thus help to reduce animal testing by setting priorities for subsequent in vivo testing.
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spelling pubmed-45515442015-09-01 Progress and future of in vitro models to study translocation of nanoparticles Braakhuis, Hedwig M. Kloet, Samantha K. Kezic, Sanja Kuper, Frieke Park, Margriet V. D. Z. Bellmann, Susann van der Zande, Meike Le Gac, Séverine Krystek, Petra Peters, Ruud J. B. Rietjens, Ivonne M. C. M. Bouwmeester, Hans Arch Toxicol Review Article The increasing use of nanoparticles in products likely results in increased exposure of both workers and consumers. Because of their small size, there are concerns that nanoparticles unintentionally cross the barriers of the human body. Several in vivo rodent studies show that, dependent on the exposure route, time, and concentration, and their characteristics, nanoparticles can cross the lung, gut, skin, and placental barrier. This review aims to evaluate the performance of in vitro models that mimic the barriers of the human body, with a focus on the lung, gut, skin, and placental barrier. For these barriers, in vitro models of varying complexity are available, ranging from single-cell-type monolayer to multi-cell (3D) models. Only a few studies are available that allow comparison of the in vitro translocation to in vivo data. This situation could change since the availability of analytical detection techniques is no longer a limiting factor for this comparison. We conclude that to further develop in vitro models to be used in risk assessment, the current strategy to improve the models to more closely mimic the human situation by using co-cultures of different cell types and microfluidic approaches to better control the tissue microenvironments are essential. At the current state of the art, the in vitro models do not yet allow prediction of absolute transfer rates but they do support the definition of relative transfer rates and can thus help to reduce animal testing by setting priorities for subsequent in vivo testing. Springer Berlin Heidelberg 2015-05-15 2015 /pmc/articles/PMC4551544/ /pubmed/25975987 http://dx.doi.org/10.1007/s00204-015-1518-5 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Review Article
Braakhuis, Hedwig M.
Kloet, Samantha K.
Kezic, Sanja
Kuper, Frieke
Park, Margriet V. D. Z.
Bellmann, Susann
van der Zande, Meike
Le Gac, Séverine
Krystek, Petra
Peters, Ruud J. B.
Rietjens, Ivonne M. C. M.
Bouwmeester, Hans
Progress and future of in vitro models to study translocation of nanoparticles
title Progress and future of in vitro models to study translocation of nanoparticles
title_full Progress and future of in vitro models to study translocation of nanoparticles
title_fullStr Progress and future of in vitro models to study translocation of nanoparticles
title_full_unstemmed Progress and future of in vitro models to study translocation of nanoparticles
title_short Progress and future of in vitro models to study translocation of nanoparticles
title_sort progress and future of in vitro models to study translocation of nanoparticles
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551544/
https://www.ncbi.nlm.nih.gov/pubmed/25975987
http://dx.doi.org/10.1007/s00204-015-1518-5
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