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Enhanced secretion of a methyl parathion hydrolase in Pichia pastoris using a combinational strategy

BACKGROUND: Although Pichia pastoris has been successfully used to produce various recombinant heterologous proteins, the efficiency varies. In this study, we used methyl parathion hydrolase (MPH) from Ochrobactrum sp. M231 as an example to study the effect of protein amino acid sequence on secretio...

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Detalles Bibliográficos
Autores principales: Wang, Ping, Huang, Lu, Jiang, Hu, Tian, Jian, Chu, Xiaoyu, Wu, Ningfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551668/
https://www.ncbi.nlm.nih.gov/pubmed/26310666
http://dx.doi.org/10.1186/s12934-015-0315-4